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@article{1318410, author = {Čejková, Darina and Strouhal, Michal and Norris, Steven J. and Weinstock, George M. and Šmajs, David}, article_location = {San Francisco}, article_number = {10}, doi = {http://dx.doi.org/10.1371/journal.pntd.0004110}, keywords = {PALLIDUM SUBSP PALLIDUM; COMPLETE GENOME SEQUENCE; BURROWS-WHEELER TRANSFORM; OUTER-MEMBRANE PROTEIN; ANTIGENIC VARIATION; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; SSP-PALLIDUM; TPRK GENE; SYPHILIS TREPONEMES}, language = {eng}, issn = {1935-2735}, journal = {PLoS Neglected Tropical Diseases}, title = {A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions}, volume = {9}, year = {2015} }
TY - JOUR ID - 1318410 AU - Čejková, Darina - Strouhal, Michal - Norris, Steven J. - Weinstock, George M. - Šmajs, David PY - 2015 TI - A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions JF - PLoS Neglected Tropical Diseases VL - 9 IS - 10 SP - 1-19 EP - 1-19 PB - Public Library of Science SN - 19352735 KW - PALLIDUM SUBSP PALLIDUM KW - COMPLETE GENOME SEQUENCE KW - BURROWS-WHEELER TRANSFORM KW - OUTER-MEMBRANE PROTEIN KW - ANTIGENIC VARIATION KW - ESCHERICHIA-COLI KW - MYCOBACTERIUM-TUBERCULOSIS KW - SSP-PALLIDUM KW - TPRK GENE KW - SYPHILIS TREPONEMES N2 - BACKGROUND: Pathogenic uncultivable treponemes comprise human and animal pathogens including agents of syphilis, yaws, bejel, pinta, and venereal spirochetosis in rabbits and hares. A set of 10 treponemal genome sequences including those of 4 Treponema pallidum ssp. pallidum (TPA) strains (Nichols, DAL-1, Mexico A, SS14), 4 T. p. ssp. pertenue (TPE) strains (CDC-2, Gauthier, Samoa D, Fribourg-Blanc), 1 T. p. ssp. endemicum (TEN) strain (Bosnia A) and one strain (Cuniculi A) of Treponema paraluisleporidarum ecovar Cuniculus (TPLC) were examined with respect to the presence of nucleotide intrastrain heterogeneous sites. METHODOLOGY/PRINCIPAL FINDINGS: The number of identified intrastrain heterogeneous sites in individual genomes ranged between 0 and 7. Altogether, 23 intrastrain heterogeneous sites (in 17 genes) were found in 5 out of 10 investigated treponemal genomes including TPA strains Nichols (n = 5), DAL-1 (n = 4), and SS14 (n = 7), TPE strain Samoa D (n = 1), and TEN strain Bosnia A (n = 5). Although only one heterogeneous site was identified among 4 tested TPE strains, 16 such sites were identified among 4 TPA strains. Heterogeneous sites were mostly strain-specific and were identified in four tpr genes (tprC, GI, I, K), in genes involved in bacterial motility and chemotaxis (fliI, cheC-fliY), in genes involved in cell structure (murC), translation (prfA), general and DNA metabolism (putative SAM dependent methyltransferase, topA), and in seven hypothetical genes. CONCLUSIONS/SIGNIFICANCE: Heterogeneous sites likely represent both the selection of adaptive changes during infection of the host as well as an ongoing diversifying evolutionary process. ER -
ČEJKOVÁ, Darina, Michal STROUHAL, Steven J. NORRIS, George M. WEINSTOCK a David ŠMAJS. A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions. \textit{PLoS Neglected Tropical Diseases}. San Francisco: Public Library of Science, 2015, roč.~9, č.~10, s.~1-19. ISSN~1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0004110.
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