A Retrospective Study on Genetic Heterogeneity within Treponema
Strains: Subpopulations Are Genetically Distinct in a Limited
Number of Positions

J 2015

A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions

ČEJKOVÁ, Darina, Michal STROUHAL, Steven J. NORRIS, George M. WEINSTOCK, David ŠMAJS et. al.

Základní údaje

Originální název

A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions

Autoři

ČEJKOVÁ, Darina (203 Česká republika, domácí), Michal STROUHAL (203 Česká republika, domácí), Steven J. NORRIS (840 Spojené státy), George M. WEINSTOCK (840 Spojené státy) a David ŠMAJS (203 Česká republika, garant, domácí)

Vydání

PLoS Neglected Tropical Diseases, San Francisco, Public Library of Science, 2015, 1935-2735

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.948

Kód RIV

RIV/00216224:14110/15:00081223

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pntd.0004110

UT WoS

000364459600023

Klíčová slova anglicky

PALLIDUM SUBSP PALLIDUM; COMPLETE GENOME SEQUENCE; BURROWS-WHEELER TRANSFORM; OUTER-MEMBRANE PROTEIN; ANTIGENIC VARIATION; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; SSP-PALLIDUM; TPRK GENE; SYPHILIS TREPONEMES

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 1. 2016 13:45, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

BACKGROUND: Pathogenic uncultivable treponemes comprise human and animal pathogens including agents of syphilis, yaws, bejel, pinta, and venereal spirochetosis in rabbits and hares. A set of 10 treponemal genome sequences including those of 4 Treponema pallidum ssp. pallidum (TPA) strains (Nichols, DAL-1, Mexico A, SS14), 4 T. p. ssp. pertenue (TPE) strains (CDC-2, Gauthier, Samoa D, Fribourg-Blanc), 1 T. p. ssp. endemicum (TEN) strain (Bosnia A) and one strain (Cuniculi A) of Treponema paraluisleporidarum ecovar Cuniculus (TPLC) were examined with respect to the presence of nucleotide intrastrain heterogeneous sites. METHODOLOGY/PRINCIPAL FINDINGS: The number of identified intrastrain heterogeneous sites in individual genomes ranged between 0 and 7. Altogether, 23 intrastrain heterogeneous sites (in 17 genes) were found in 5 out of 10 investigated treponemal genomes including TPA strains Nichols (n = 5), DAL-1 (n = 4), and SS14 (n = 7), TPE strain Samoa D (n = 1), and TEN strain Bosnia A (n = 5). Although only one heterogeneous site was identified among 4 tested TPE strains, 16 such sites were identified among 4 TPA strains. Heterogeneous sites were mostly strain-specific and were identified in four tpr genes (tprC, GI, I, K), in genes involved in bacterial motility and chemotaxis (fliI, cheC-fliY), in genes involved in cell structure (murC), translation (prfA), general and DNA metabolism (putative SAM dependent methyltransferase, topA), and in seven hypothetical genes. CONCLUSIONS/SIGNIFICANCE: Heterogeneous sites likely represent both the selection of adaptive changes during infection of the host as well as an ongoing diversifying evolutionary process.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

GAP302/12/0574, projekt VaV

Název: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Grantová agentura ČR, Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

GP14-29596P, projekt VaV

Název: Genomová variabilita treponem během experimentální infekce

Investor: Grantová agentura ČR, Genomová variabilita treponem během experimentální infekce

NT11159, projekt VaV

Název: Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Investor: Ministerstvo zdravotnictví ČR, Mapování výskytu makrolidové rezistence původce syfilis v ČR a molekulární typizace jednotlivých syfilitických kmenů

Citovat

ČEJKOVÁ, Darina, Michal STROUHAL, Steven J. NORRIS, George M. WEINSTOCK a David ŠMAJS. A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions. PLoS Neglected Tropical Diseases. San Francisco: Public Library of Science, 2015, roč. 9, č. 10, s. 1-19. ISSN 1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0004110.

@article{1318410,
   author = {Čejková, Darina and Strouhal, Michal and Norris, Steven J. and Weinstock, George M. and Šmajs, David},
   article_location = {San Francisco},
   article_number = {10},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0004110},
   keywords = {PALLIDUM SUBSP PALLIDUM; COMPLETE GENOME SEQUENCE; BURROWS-WHEELER TRANSFORM; OUTER-MEMBRANE PROTEIN; ANTIGENIC VARIATION; ESCHERICHIA-COLI; MYCOBACTERIUM-TUBERCULOSIS; SSP-PALLIDUM; TPRK GENE; SYPHILIS TREPONEMES},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS Neglected Tropical Diseases},
   title = {A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions},
   volume = {9},
   year = {2015}
}

TY  - JOUR
ID  - 1318410
AU  - Čejková, Darina - Strouhal, Michal - Norris, Steven J. - Weinstock, George M. - Šmajs, David
PY  - 2015
TI  - A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions
JF  - PLoS Neglected Tropical Diseases
VL  - 9
IS  - 10
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19352735
KW  - PALLIDUM SUBSP PALLIDUM
KW  - COMPLETE GENOME SEQUENCE
KW  - BURROWS-WHEELER TRANSFORM
KW  - OUTER-MEMBRANE PROTEIN
KW  - ANTIGENIC VARIATION
KW  - ESCHERICHIA-COLI
KW  - MYCOBACTERIUM-TUBERCULOSIS
KW  - SSP-PALLIDUM
KW  - TPRK GENE
KW  - SYPHILIS TREPONEMES
N2  - BACKGROUND: Pathogenic uncultivable treponemes comprise human and animal pathogens including agents of syphilis, yaws, bejel, pinta, and venereal spirochetosis in rabbits and hares. A set of 10 treponemal genome sequences including those of 4 Treponema pallidum ssp. pallidum (TPA) strains (Nichols, DAL-1, Mexico A, SS14), 4 T. p. ssp. pertenue (TPE) strains (CDC-2, Gauthier, Samoa D, Fribourg-Blanc), 1 T. p. ssp. endemicum (TEN) strain (Bosnia A) and one strain (Cuniculi A) of Treponema paraluisleporidarum ecovar Cuniculus (TPLC) were examined with respect to the presence of nucleotide intrastrain heterogeneous sites. METHODOLOGY/PRINCIPAL FINDINGS: The number of identified intrastrain heterogeneous sites in individual genomes ranged between 0 and 7. Altogether, 23 intrastrain heterogeneous sites (in 17 genes) were found in 5 out of 10 investigated treponemal genomes including TPA strains Nichols (n = 5), DAL-1 (n = 4), and SS14 (n = 7), TPE strain Samoa D (n = 1), and TEN strain Bosnia A (n = 5). Although only one heterogeneous site was identified among 4 tested TPE strains, 16 such sites were identified among 4 TPA strains. Heterogeneous sites were mostly strain-specific and were identified in four tpr genes (tprC, GI, I, K), in genes involved in bacterial motility and chemotaxis (fliI, cheC-fliY), in genes involved in cell structure (murC), translation (prfA), general and DNA metabolism (putative SAM dependent methyltransferase, topA), and in seven hypothetical genes. CONCLUSIONS/SIGNIFICANCE: Heterogeneous sites likely represent both the selection of adaptive changes during infection of the host as well as an ongoing diversifying evolutionary process.
ER  -

ČEJKOVÁ, Darina, Michal STROUHAL, Steven J. NORRIS, George M. WEINSTOCK a David ŠMAJS. A Retrospective Study on Genetic Heterogeneity within Treponema Strains: Subpopulations Are Genetically Distinct in a Limited Number of Positions. \textit{PLoS Neglected Tropical Diseases}. San Francisco: Public Library of Science, 2015, roč.~9, č.~10, s.~1-19. ISSN~1935-2735. Dostupné z: https://dx.doi.org/10.1371/journal.pntd.0004110.

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