Detailed Information on Publication Record
2015
Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species
CAO, Hieu X., Thomas SCHMUTZER, Uwe SCHOLZ, Aleš PEČINKA, Ingo SCHUBERT et. al.Basic information
Original name
Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species
Authors
CAO, Hieu X. (276 Germany), Thomas SCHMUTZER (276 Germany), Uwe SCHOLZ (276 Germany), Aleš PEČINKA (203 Czech Republic), Ingo SCHUBERT (276 Germany, belonging to the institution) and Giang T. H. VU (276 Germany)
Edition
Frontiers in Microbiology, Lausanne (Switzerland), Frontiers Media SA, 2015, 1664-302X
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.165
RIV identification code
RIV/00216224:14740/15:00084835
Organization unit
Central European Institute of Technology
UT WoS
000358573200001
Keywords in English
Genlisea; plant carnivory; lobster pot trapping; metatranscriptomics; RNA-sequencing; whole-genome gene transcription analysis; algae commensalism; plant-microbe interaction
Tags
International impact, Reviewed
Změněno: 4/4/2016 10:37, Mgr. Eva Špillingová
Abstract
V originále
In the carnivorous plant genus Genlisea a unique lobster pot trapping mechanism supplements nutrition in nutrient-poor habitats. A wide spectrum of microbes frequently occurs in Genlisea's leaf-derived traps without clear relevance for Genlisea carnivory. We sequenced the metatranscriptomes of subterrestrial traps vs. the aerial chlorophyll-containing leaves of G. nigrocaulis and of G. hispidula. Ribosomal RNA assignment revealed soil-borne microbial diversity in Genlisea traps, with 92 genera of 19 phyla present in more than one sample. Microbes from 16 of these phyla including proteobacteria, green algae, amoebozoa, fungi, ciliates and metazoans, contributed additionally short-lived mRNA to the metatranscriptome. Furthermore, transcripts of 438 members of hydrolases (e.g., proteases, phosphatases, lipases), mainly resembling those of metazoans, ciliates and green algae, were found. Compared to aerial leaves, Genlisea traps displayed a transcriptional up-regulation of endogenous NADH oxidases generating reactive oxygen species as well as of acid phosphatases for prey digestion. A leaf-vs.-trap transcriptome comparison reflects that carnivory provides inorganic P- and different forms of N-compounds (ammonium, nitrate, amino acid, oligopeptides) and implies the need to protect trap cells against oxidative stress. The analysis elucidates a complex food web inside the Genlisea traps, and suggests ecological relationships between this plant genus and its entrapped microbiome.
Links
EE2.3.20.0189, research and development project |
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