Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea ...

@article{1318476,
   author = {Cao, Hieu X. and Schmutzer, Thomas and Scholz, Uwe and Pečinka, Aleš and Schubert, Ingo and Vu, Giang T. H.},
   article_location = {Lausanne (Switzerland)},
   article_number = {july},
   doi = {http://dx.doi.org/10.3389/fmicb.2015.00526},
   keywords = {Genlisea; plant carnivory; lobster pot trapping; metatranscriptomics; RNA-sequencing; whole-genome gene transcription analysis; algae commensalism; plant-microbe interaction},
   language = {eng},
   issn = {1664-302X},
   journal = {Frontiers in Microbiology},
   title = {Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species},
   url = {http://journal.frontiersin.org/article/10.3389/fmicb.2015.00526/abstract},
   volume = {6},
   year = {2015}
}

TY  - JOUR
ID  - 1318476
AU  - Cao, Hieu X. - Schmutzer, Thomas - Scholz, Uwe - Pečinka, Aleš - Schubert, Ingo - Vu, Giang T. H.
PY  - 2015
TI  - Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species
JF  - Frontiers in Microbiology
VL  - 6
IS  - july
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - Frontiers Media SA
SN  - 1664302X
KW  - Genlisea
KW  - plant carnivory
KW  - lobster pot trapping
KW  - metatranscriptomics
KW  - RNA-sequencing
KW  - whole-genome gene transcription analysis
KW  - algae commensalism
KW  - plant-microbe interaction
UR  - http://journal.frontiersin.org/article/10.3389/fmicb.2015.00526/abstract
L2  - http://journal.frontiersin.org/article/10.3389/fmicb.2015.00526/abstract
N2  - In the carnivorous plant genus Genlisea a unique lobster pot trapping mechanism supplements nutrition in nutrient-poor habitats. A wide spectrum of microbes frequently occurs in Genlisea's leaf-derived traps without clear relevance for Genlisea carnivory. We sequenced the metatranscriptomes of subterrestrial traps vs. the aerial chlorophyll-containing leaves of G. nigrocaulis and of G. hispidula. Ribosomal RNA assignment revealed soil-borne microbial diversity in Genlisea traps, with 92 genera of 19 phyla present in more than one sample. Microbes from 16 of these phyla including proteobacteria, green algae, amoebozoa, fungi, ciliates and metazoans, contributed additionally short-lived mRNA to the metatranscriptome. Furthermore, transcripts of 438 members of hydrolases (e.g., proteases, phosphatases, lipases), mainly resembling those of metazoans, ciliates and green algae, were found. Compared to aerial leaves, Genlisea traps displayed a transcriptional up-regulation of endogenous NADH oxidases generating reactive oxygen species as well as of acid phosphatases for prey digestion. A leaf-vs.-trap transcriptome comparison reflects that carnivory provides inorganic P- and different forms of N-compounds (ammonium, nitrate, amino acid, oligopeptides) and implies the need to protect trap cells against oxidative stress. The analysis elucidates a complex food web inside the Genlisea traps, and suggests ecological relationships between this plant genus and its entrapped microbiome.
ER  - 

CAO, Hieu X., Thomas SCHMUTZER, Uwe SCHOLZ, Aleš PEČINKA, Ingo SCHUBERT a Giang T. H. VU. Metatranscriptome analysis reveals host-microbiome interactions in traps of carnivorous Genlisea species. \textit{Frontiers in Microbiology}. Lausanne (Switzerland): Frontiers Media SA, 2015, roč.~6, july, s.~''nestránkováno'', 15 s. ISSN~1664-302X. Dostupné z: https://dx.doi.org/10.3389/fmicb.2015.00526.