Evolution of diploid-polyploid Carassius auratus complex

k 2015

Evolution of diploid-polyploid Carassius auratus complex

PAKOSTA, Tomáš; Libor VOJTEK; Pavel HYRŠL; Lukáš VETEŠNÍK; Andrea VETEŠNÍKOVÁ ŠIMKOVÁ et. al.

Základní údaje

Originální název

Evolution of diploid-polyploid Carassius auratus complex

Název česky

Evoluce diploidně polyploidního komplexu Carassius auratus

Autoři

PAKOSTA, Tomáš (203 Česká republika, domácí); Libor VOJTEK (203 Česká republika); Pavel HYRŠL (203 Česká republika); Lukáš VETEŠNÍK (203 Česká republika) a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Česká republika, garant)

Vydání

4th Workshop of European Centre of IchthyoParasitology (ECIP), 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/15:00087745

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8016-4

Klíčová slova česky

Carassius auratus komplex; parazité; imunita; diploidní; polyploidní; D loop

Klíčová slova anglicky

Carassius auratus complex; parasites; immunity; diploid; polyploid; D loop

Změněno: 19. 12. 2016 16:04, Mgr. Tomáš Pakosta

Anotace

V originále

The presence of diploid and triploid forms of Carassius auratus complex in the Czech Republic is known for over 40 years. This complex consists of gynogenetic triploid females (triploid males are also recorded in very small frequency) and sexually reproducing form including males and females with similar frequencies. In this study, the C. auratus complex was studied from the selected lokality in the connecting channels Lednice-South Moravia situated on the Dyje river. Using molecular analysis of mitochondrial DNA (D-loop region) the collected specimens of the complex were determined as "subspecies" of Carassius auratus, C. gibelio, C. landsdorfi and M-line. The majority of specimen belong to the C. gibelio haplotype. We focused on selected measured of physiology and immunity including the specific immunity (IgM antibodies) and non-specific immunity (i.e. respiratory burst, lysozyme activity and complement activity). We also compared the haematological parameters and the level of parasite infection between two life strategies (sexual diploids versus gynogenetic polyploids) of Carassius auratus complex. Samples were collected in August during three consecutive years. Our analyses revealed no difference in non-specific immunity between two reproductive forms but the difference in IgM production between gynogens and sexuals was found. Concerning the haematological parameters, only erythrocyte count and hematocrit was clearly affected by ploidy status. High investment in the reproduction was found for both gynogenetic and sexual females when compared to sexual males. No obvious difference was found in the abundance of different parasite group between diploid sexuals and triploid gynogens.

Návaznosti

GAP505/12/0375, projekt VaV

Název: Evolučně-imunitní a ekologické aspekty parazitace u hybridních a polyploidních kaprovitých ryb

Investor: Grantová agentura ČR, Evolučně-imunitní a ekologické aspekty parazitace u hybridních a polypoidních kaprovitých ryb

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

PAKOSTA, Tomáš; Libor VOJTEK; Pavel HYRŠL; Lukáš VETEŠNÍK a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. Evolution of diploid-polyploid Carassius auratus complex. In 4th Workshop of European Centre of IchthyoParasitology (ECIP). 2015. ISBN 978-80-210-8016-4.

@proceedings{1318765,
   author = {Pakosta, Tomáš and Vojtek, Libor and Hyršl, Pavel and Vetešník, Lukáš and Vetešníková Šimková, Andrea},
   booktitle = {4th Workshop of European Centre of IchthyoParasitology (ECIP)},
   keywords = {Carassius auratus complex; parasites; immunity; diploid; polyploid; D loop},
   language = {eng},
   isbn = {978-80-210-8016-4},
   title = {Evolution of diploid-polyploid Carassius auratus complex},
   year = {2015}
}

TY  - CONF
ID  - 1318765
AU  - Pakosta, Tomáš - Vojtek, Libor - Hyršl, Pavel - Vetešník, Lukáš - Vetešníková Šimková, Andrea
PY  - 2015
TI  - Evolution of diploid-polyploid Carassius auratus complex
SN  - 9788021080164
KW  - Carassius auratus complex
KW  - parasites
KW  - immunity
KW  - diploid
KW  - polyploid
KW  - D loop
N2  - The presence of diploid and triploid forms of Carassius auratus complex in the Czech Republic is known for over 40 years. This complex consists of gynogenetic triploid females (triploid males are also recorded in very small frequency) and sexually reproducing form including males and females with similar frequencies. In this study, the C. auratus complex was studied from the selected lokality in the connecting channels Lednice-South Moravia situated on the Dyje river. Using molecular analysis of mitochondrial DNA (D-loop region) the collected specimens of the complex were determined as "subspecies" of Carassius auratus, C. gibelio, C. landsdorfi and M-line. The majority of specimen belong to the C. gibelio haplotype. We focused on selected measured of physiology and immunity including the specific immunity (IgM antibodies) and non-specific immunity (i.e. respiratory burst, lysozyme activity and complement activity). We also compared the haematological parameters and the level of parasite infection between two life strategies (sexual diploids versus gynogenetic polyploids) of Carassius auratus complex. Samples were collected in August during three consecutive years. Our analyses revealed no difference in non-specific immunity between two reproductive forms but the difference in IgM production between gynogens and sexuals was found. Concerning the haematological parameters, only erythrocyte count and hematocrit was clearly affected by ploidy status. High investment in the reproduction was found for both gynogenetic and sexual females when compared to sexual males. No obvious difference was found in the abundance of different parasite group between diploid sexuals and triploid gynogens.
ER  -

PAKOSTA, Tomáš; Libor VOJTEK; Pavel HYRŠL; Lukáš VETEŠNÍK a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. Evolution of diploid-polyploid Carassius auratus complex. In \textit{4th Workshop of European Centre of IchthyoParasitology (ECIP)}. 2015. ISBN~978-80-210-8016-4.

Přehled o publikaci