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@article{1318873,
author = {Sedláček, Ivo and Grillová, Linda and Staňková, Eva and Černohlávková, Jitka and Šmajs, David},
article_number = {2},
keywords = {identification; bacteriocin; biotyping; ribotyping},
language = {eng},
issn = {1805-0689},
journal = {Czech polar Reports},
title = {Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica},
volume = {3},
year = {2013}
}
TY - JOUR
ID - 1318873
AU - Sedláček, Ivo - Grillová, Linda - Staňková, Eva - Černohlávková, Jitka - Šmajs, David
PY - 2013
TI - Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica
JF - Czech polar Reports
VL - 3
IS - 2
SP - 173-183
EP - 173-183
SN - 18050689
KW - identification
KW - bacteriocin
KW - biotyping
KW - ribotyping
N2 - A set of nine gram-negative fermenting rods biochemically identified as Escherichia coli was isolated from faeces of seals. These bacteria were characterized by phenotypic classification, 16S rDNA sequence analyses, automated ribotyping, study of whole-cell protein profiles by SDS-PAGE and finally by bacteriocin production. The results of our polyphasic taxonomic study supported the recognition of P4652, P4653 and P4740 isolates as true members of Escherichia albertii species – probably a major enteric human pathogen. To our best knowledge, this is the first evidence showing that E. albertii produces bacteriocin, and colicin D. Obtained data unambiguously showed inconvenience of commercial identification systems to distinguish both Escherichia species due to missing data of E. albertii in the commercial databases. The results of Escherichia isolates taxonomy suggest seals as a novel source of human and animal pathogen, E. albertii in the Antarctic region.
ER -
SEDLÁČEK, Ivo, Linda GRILLOVÁ, Eva STAŇKOVÁ, Jitka ČERNOHLÁVKOVÁ a David ŠMAJS. Isolation of human pathogen Escherichia albertii from faeces of seals (Leptonychos weddelli) in James Ross Island, Antarctica. \textit{Czech polar Reports}. 2013, roč.~3, č.~2, s.~173-183. ISSN~1805-0689.
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