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@article{1319734, author = {Kaspar, Petr and Zikova, Martina and Bartunek, Petr and Štěrba, Jaroslav and Strnad, Hynek and Křen, Leoš and Sedlacek, Radislav}, article_location = {London}, article_number = {15090}, doi = {http://dx.doi.org/10.1038/srep15090}, keywords = {CELL-DIFFERENTIATION; PEDIATRIC RHABDOMYOSARCOMAS; GENE-EXPRESSION; DOWN-REGULATION; CANCER-CELLS; PROLIFERATION; MICRORNAS; SURVIVAL; LEUKEMIA; INVASION}, language = {eng}, issn = {2045-2322}, journal = {Scientific Reports}, title = {The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin}, volume = {5}, year = {2015} }
TY - JOUR ID - 1319734 AU - Kaspar, Petr - Zikova, Martina - Bartunek, Petr - Štěrba, Jaroslav - Strnad, Hynek - Křen, Leoš - Sedlacek, Radislav PY - 2015 TI - The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin JF - Scientific Reports VL - 5 IS - 15090 SP - 1-10 EP - 1-10 PB - Nature Publishing Group SN - 20452322 KW - CELL-DIFFERENTIATION KW - PEDIATRIC RHABDOMYOSARCOMAS KW - GENE-EXPRESSION KW - DOWN-REGULATION KW - CANCER-CELLS KW - PROLIFERATION KW - MICRORNAS KW - SURVIVAL KW - LEUKEMIA KW - INVASION N2 - The transcription factor c-Myb is required for modulation of progenitor cells in several tissues, including skeletal muscle and its upregulation is observed in many human malignancies. Rhabdomyosarcomas (RMS) are a heterogeneous group of mesodermal tumors with features of developing skeletal muscle. Several miRNAs are downregulated in RMS, including miR-150, a negative regulator of c-Myb expression. Using the C2C12 myoblast cell line, a cellular model of skeletal muscle differentiation, we showed that miR-150 controls c-Myb expression mainly at the level of translation. We hypothesized that a similar mechanism of c-Myb regulation operates in RMS tumors. We examined expression of c-Myb by immunohistochemistry and revealed c-Myb positivity in alveolar and embryonal tumors, the two most common subgroups of RMS. Furthermore, we showed direct correlation between c-Myb production and myogenin expression. Interestingly, high myogenin levels indicate poor prognosis in RMS patients. c-Myb could, therefore, contribute to the tumor phenotype by executing its inhibitory role in skeletal muscle differentiation. We also showed that c-Myb protein is abundant in migratory C2C12 myoblasts and its ectopic expression potentiates cell motility. In summary, our results implicate that metastatic properties of some RMS subtypes might be linked to c-Myb function. ER -
KASPAR, Petr, Martina ZIKOVA, Petr BARTUNEK, Jaroslav ŠTĚRBA, Hynek STRNAD, Leoš KŘEN a Radislav SEDLACEK. The Expression of c-Myb Correlates with the Levels of Rhabdomyosarcoma-specific Marker Myogenin. \textit{Scientific Reports}. London: Nature Publishing Group, 2015, roč.~5, č.~15090, s.~1-10. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep15090.
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