Molecular response of 4T1-induced mouse mammary tumours and
healthy tissues to zinc treatment

J 2015

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ et. al.

Základní údaje

Originální název

Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment

Autoři

SVOBODOVÁ, Markéta (203 Česká republika, domácí), Jaromír GUMULEC (203 Česká republika, domácí), Martina RAUDENSKÁ (203 Česká republika, domácí), Hana POLANSKÁ (203 Česká republika, domácí), Monika HOLUBOVÁ (203 Česká republika, domácí), Jan BALVAN (203 Česká republika, domácí), Kristýna HUDCOVÁ (203 Česká republika, domácí), Lucia KNOPFOVÁ (203 Česká republika), René KIZEK (203 Česká republika), Vojtech ADAM (203 Česká republika), Petr BABULA (203 Česká republika, domácí) a Michal MASAŘÍK (203 Česká republika, garant, domácí)

Vydání

International Journal of Oncology, Athens, Spandidos Publications, 2015, 1019-6439

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.018

Kód RIV

RIV/00216224:14110/15:00085106

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.3892/ijo.2015.2883

UT WoS

000350616400042

Klíčová slova anglicky

breast cancer; zinc treatment; tumour size; metallothionein; glutathione

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 12. 2015 15:52, Soňa Böhmová

Anotace

V originále

Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.

Návaznosti

MUNI/A/1003/2013, interní kód MU

Název: Molekulární patofyziologie vybraných komplexních nemocí

Investor: Masarykova univerzita, Molekulární patofyziologie vybraných komplexních nemocí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/24/LF5/2014, interní kód MU

Název: Ovlivnění metabolismu nádorových buněk jako nový nástroj cílené terapie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Ovlivnění metabolismu nádorových buněk jako nový nástroj cílené terapie

Citovat

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ, Jan BALVAN, Kristýna HUDCOVÁ, Lucia KNOPFOVÁ, René KIZEK, Vojtech ADAM, Petr BABULA a Michal MASAŘÍK. Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment. International Journal of Oncology. Athens: Spandidos Publications, 2015, roč. 46, č. 4, s. 1810-1818. ISSN 1019-6439. Dostupné z: https://dx.doi.org/10.3892/ijo.2015.2883.

@article{1320371,
   author = {Svobodová, Markéta and Gumulec, Jaromír and Raudenská, Martina and Polanská, Hana and Holubová, Monika and Balvan, Jan and Hudcová, Kristýna and Knopfová, Lucia and Kizek, René and Adam, Vojtech and Babula, Petr and Masařík, Michal},
   article_location = {Athens},
   article_number = {4},
   doi = {http://dx.doi.org/10.3892/ijo.2015.2883},
   keywords = {breast cancer; zinc treatment; tumour size; metallothionein; glutathione},
   language = {eng},
   issn = {1019-6439},
   journal = {International Journal of Oncology},
   title = {Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment},
   volume = {46},
   year = {2015}
}

TY  - JOUR
ID  - 1320371
AU  - Svobodová, Markéta - Gumulec, Jaromír - Raudenská, Martina - Polanská, Hana - Holubová, Monika - Balvan, Jan - Hudcová, Kristýna - Knopfová, Lucia - Kizek, René - Adam, Vojtech - Babula, Petr - Masařík, Michal
PY  - 2015
TI  - Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment
JF  - International Journal of Oncology
VL  - 46
IS  - 4
SP  - 1810-1818
EP  - 1810-1818
PB  - Spandidos Publications
SN  - 10196439
KW  - breast cancer
KW  - zinc treatment
KW  - tumour size
KW  - metallothionein
KW  - glutathione
N2  - Breast cancer patients negative for the nuclear oestrogen receptor alpha have a particularly poor prognosis. Therefore, the 4T1 cell line (considered as a triple-negative model) was chosen to induce malignancy in mice. The aim of the present study was to assess if zinc ions, provided in excess, may significantly modify the process of mammary oncogenesis. Zn(II) ions were chosen because of their documented antitumour effects. Zn(II) is also known to induce the expression of metallothioneins (MT) and glutathion (GSH). A total dose of zinc sulphate per one gram of mouse weight used in the experiment was 0.15 mg. We studied the expression of MT1 MT2, TP53 and MTF-1genes and also examined the effect of the tumour on antioxidant capacity. Tumour-free mice had significantly higher expression levels of the studied genes (P<0.003). Significant differences were also revealed in the gene expression between the tissues (P<0.001). The highest expression levels were observed in the liver. As compared to brain, lung and liver, significantly lower concentrations of MT protein were found in the primary tumour; an inverse trend was observed in the concentration of Zinc(II). In non-tumour mice, the amount of hepatic hydrosulphuryl groups significantly increased by the exposure to Zn(II), but the animals with tumour induction showed no similar trend. The primary tumour size of zinc-treated animals was 20% smaller (P=0.002); however, no significant effect on metastasis progression due to the zinc treatment was discovered. In conclusion, Zn(II) itself may mute the growth of primary breast tumours especially at their early stages.
ER  -

SVOBODOVÁ, Markéta, Jaromír GUMULEC, Martina RAUDENSKÁ, Hana POLANSKÁ, Monika HOLUBOVÁ, Jan BALVAN, Kristýna HUDCOVÁ, Lucia KNOPFOVÁ, René KIZEK, Vojtech ADAM, Petr BABULA a Michal MASAŘÍK. Molecular response of 4T1-induced mouse mammary tumours and healthy tissues to zinc treatment. \textit{International Journal of Oncology}. Athens: Spandidos Publications, 2015, roč.~46, č.~4, s.~1810-1818. ISSN~1019-6439. Dostupné z: https://dx.doi.org/10.3892/ijo.2015.2883.

Podrobný výpis o publikaci