Identification of Spen as a Crucial Factor for Xist Function
through Forward Genetic Screening in Haploid Embryonic Stem
Cells

J 2015

Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells

MONFORT, Asun; Giulio DI MININ; Andreas POSTLMAYR; R. FREIMANN; Fabiana ARIETI et al.

Základní údaje

Originální název

Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells

Autoři

MONFORT, Asun; Giulio DI MININ; Andreas POSTLMAYR; R. FREIMANN; Fabiana ARIETI; Stéphane THORE a Anton WUTZ

Vydání

Cell Reports, CAMBRIDGE, Cell Press, 2015, 2211-1247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.870

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/15:00085377

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

INACTIVE X-CHROMOSOME; DOSAGE COMPENSATION; SAF-A; RNA; SHARP; MICE; LOCALIZATION; MAINTENANCE; ACTIVATION; SUPPRESSOR

Štítky

OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 4. 2016 10:11, Mgr. Eva Špillingová

Anotace

V originále

In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X chromosomes in female cells. Here, we report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells (ESCs) that carry an engineered selectable reporter system. This system was able to identify several candidate factors that are genetically required for chromosomal repression by Xist. Among the list of candidates, we identify the RNA-binding protein Spen, the homolog of split ends. Independent validation through gene deletion in ESCs confirms that Spen is required for gene repression by Xist. However, Spen is not required for Xist RNA localization and the recruitment of chromatin modifications, including Polycomb protein Ezh2. The identification of Spen opens avenues for further investigation into the gene-silencing pathway of Xist and shows the usefulness of haploid ESCs for genetic screening of epigenetic pathways.

Přiložené soubory

1322058_Identification_of_Spen.pdf

Požádat o autorskou verzi souboru

Citovat

MONFORT, Asun; Giulio DI MININ; Andreas POSTLMAYR; R. FREIMANN; Fabiana ARIETI; Stéphane THORE a Anton WUTZ. Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells. Cell Reports. CAMBRIDGE: Cell Press, 2015, roč. 12, č. 4, s. 554-561. ISSN 2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2015.06.067.

@article{1322058,
   author = {Monfort, Asun and Di Minin, Giulio and Postlmayr, Andreas and Freimann, R. and Arieti, Fabiana and Thore, Stéphane and Wutz, Anton},
   article_location = {CAMBRIDGE},
   article_number = {4},
   doi = {https://doi.org/10.1016/j.celrep.2015.06.067},
   keywords = {INACTIVE X-CHROMOSOME; DOSAGE COMPENSATION; SAF-A; RNA; SHARP; MICE; LOCALIZATION; MAINTENANCE; ACTIVATION; SUPPRESSOR},
   language = {eng},
   issn = {2211-1247},
   journal = {Cell Reports},
   title = {Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells},
   url = {http://ac.els-cdn.com/S2211124715007056/1-s2.0-S2211124715007056-main.pdf?_tid=b2a09f7c-a955-11e5-8086-00000aab0f6c&acdnat=1450862306_2c1bb6f674674ff62a019a857bbab0ea},
   volume = {12},
   year = {2015}
}

TY  - JOUR
ID  - 1322058
AU  - Monfort, Asun - Di Minin, Giulio - Postlmayr, Andreas - Freimann, R. - Arieti, Fabiana - Thore, Stéphane - Wutz, Anton
PY  - 2015
TI  - Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells
JF  - Cell Reports
VL  - 12
IS  - 4
SP  - 554-561
EP  - 554-561
PB  - Cell Press
SN  - 22111247
KW  - INACTIVE X-CHROMOSOME
KW  - DOSAGE COMPENSATION
KW  - SAF-A
KW  - RNA
KW  - SHARP
KW  - MICE
KW  - LOCALIZATION
KW  - MAINTENANCE
KW  - ACTIVATION
KW  - SUPPRESSOR
UR  - http://ac.els-cdn.com/S2211124715007056/1-s2.0-S2211124715007056-main.pdf?_tid=b2a09f7c-a955-11e5-8086-00000aab0f6c&acdnat=1450862306_2c1bb6f674674ff62a019a857bbab0ea
L2  - http://ac.els-cdn.com/S2211124715007056/1-s2.0-S2211124715007056-main.pdf?_tid=b2a09f7c-a955-11e5-8086-00000aab0f6c&acdnat=1450862306_2c1bb6f674674ff62a019a857bbab0ea
N2  - In mammals, the noncoding Xist RNA triggers transcriptional silencing of one of the two X chromosomes in female cells. Here, we report a genetic screen for silencing factors in X chromosome inactivation using haploid mouse embryonic stem cells (ESCs) that carry an engineered selectable reporter system. This system was able to identify several candidate factors that are genetically required for chromosomal repression by Xist. Among the list of candidates, we identify the RNA-binding protein Spen, the homolog of split ends. Independent validation through gene deletion in ESCs confirms that Spen is required for gene repression by Xist. However, Spen is not required for Xist RNA localization and the recruitment of chromatin modifications, including Polycomb protein Ezh2. The identification of Spen opens avenues for further investigation into the gene-silencing pathway of Xist and shows the usefulness of haploid ESCs for genetic screening of epigenetic pathways.
ER  -

MONFORT, Asun; Giulio DI MININ; Andreas POSTLMAYR; R. FREIMANN; Fabiana ARIETI; Stéphane THORE a Anton WUTZ. Identification of Spen as a Crucial Factor for Xist Function through Forward Genetic Screening in Haploid Embryonic Stem Cells. \textit{Cell Reports}. CAMBRIDGE: Cell Press, 2015, roč.~12, č.~4, s.~554-561. ISSN~2211-1247. Dostupné z: https://doi.org/10.1016/j.celrep.2015.06.067.

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