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@article{1322765,
author = {Hároníková, Lucia and Špaček, Jan and Plucnara, Medard and Horáková, Petra and Pivoňková, Hana and Havran, Luděk and Erdem, Arzum and Fojta, Miroslav},
article_location = {Wien},
article_number = {5},
doi = {http://dx.doi.org/10.1007/s00706-015-1436-5},
keywords = {Enzymes; Nucleic acids; Voltammetry},
language = {eng},
issn = {0026-9247},
journal = {Monatshefte fur Chemie},
title = {Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes},
url = {http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14},
volume = {146},
year = {2015}
}
TY - JOUR
ID - 1322765
AU - Hároníková, Lucia - Špaček, Jan - Plucnara, Medard - Horáková, Petra - Pivoňková, Hana - Havran, Luděk - Erdem, Arzum - Fojta, Miroslav
PY - 2015
TI - Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes
JF - Monatshefte fur Chemie
VL - 146
IS - 5
SP - 849-855
EP - 849-855
PB - Springer-Verlag
SN - 00269247
KW - Enzymes
KW - Nucleic acids
KW - Voltammetry
UR - http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14
L2 - http://download.springer.com/static/pdf/109/art%253A10.1007%252Fs00706-015-1436-5.pdf?originUrl=http%3A%2F%2Flink.springer.com%2Farticle%2F10.1007%2Fs00706-015-1436-5&token2=exp=1451982708~acl=%2Fstatic%2Fpdf%2F109%2Fart%25253A10.1007%25252Fs00706-015-14
N2 - In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin-alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and its applicability for off-line monitoring of DNA amplification is demonstrated. Applications of the technique include detection of the presence of specific nucleotide sequences in biological samples, such as sequences related to pathogenic microorganism or transgenes. [GRAPHICS] .
ER -
HÁRONÍKOVÁ, Lucia, Jan ŠPAČEK, Medard PLUCNARA, Petra HORÁKOVÁ, Hana PIVOŇKOVÁ, Luděk HAVRAN, Arzum ERDEM and Miroslav FOJTA. Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes. \textit{Monatshefte fur Chemie}. Wien: Springer-Verlag, 2015, vol.~146, No~5, p.~849-855. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-015-1436-5.
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