Cell cycle-dependent changes in H3K56ac in human cells.

J 2015

Cell cycle-dependent changes in H3K56ac in human cells.

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ et. al.

Základní údaje

Originální název

Cell cycle-dependent changes in H3K56ac in human cells.

Autoři

STEJSKAL, Stanislav (203 Česká republika, domácí), Karel ŠTĚPKA (203 Česká republika, domácí), Lenka TESAŘOVÁ (203 Česká republika, domácí), Karel STEJSKAL (203 Česká republika, domácí), Martina MATĚJKOVÁ (203 Česká republika, domácí), Pavel ŠIMARA (203 Česká republika, domácí), Zbyněk ZDRÁHAL (203 Česká republika, domácí) a Irena KRONTORÁD KOUTNÁ (203 Česká republika, garant, domácí)

Vydání

Cell Cycle, Philadelphia, Taylor&Francis Inc, 2015, 1538-4101

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.952

Kód RIV

RIV/00216224:14330/15:00081398

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1080/15384101.2015.1106760

UT WoS

000367063200017

Klíčová slova anglicky

Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome

Štítky

cbia-web, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 12. 2019 16:41, Mgr. Marie Šípková, DiS.

Anotace

V originále

The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.

Návaznosti

CZ.1.07/2.3.00/30.0030, interní kód MU

Název: Rozvoj lidských zdrojů pro oblast buněčné biologie

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Rozvoj lidských zdrojů pro oblast buněčné biologie, 2.3 Lidské zdroje ve výzkumu a vývoji

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GBP206/12/G151, projekt VaV

Název: Centrum nových přístupů k bioanalýze a molekulární diagnostice

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel ŠIMARA, Zbyněk ZDRÁHAL a Irena KRONTORÁD KOUTNÁ. Cell cycle-dependent changes in H3K56ac in human cells. Cell Cycle. Philadelphia: Taylor&Francis Inc, 2015, roč. 14, č. 24, s. 3851-3863. ISSN 1538-4101. Dostupné z: https://dx.doi.org/10.1080/15384101.2015.1106760.

@article{1322928,
   author = {Stejskal, Stanislav and Štěpka, Karel and Tesařová, Lenka and Stejskal, Karel and Matějková, Martina and Šimara, Pavel and Zdráhal, Zbyněk and Krontorád Koutná, Irena},
   article_location = {Philadelphia},
   article_number = {24},
   doi = {http://dx.doi.org/10.1080/15384101.2015.1106760},
   keywords = {Cell cycle; Chromatin; DNA replication; H3K56ac; Mammalian cells; Nucleosome},
   language = {eng},
   issn = {1538-4101},
   journal = {Cell Cycle},
   title = {Cell cycle-dependent changes in H3K56ac in human cells.},
   url = {http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760},
   volume = {14},
   year = {2015}
}

TY  - JOUR
ID  - 1322928
AU  - Stejskal, Stanislav - Štěpka, Karel - Tesařová, Lenka - Stejskal, Karel - Matějková, Martina - Šimara, Pavel - Zdráhal, Zbyněk - Krontorád Koutná, Irena
PY  - 2015
TI  - Cell cycle-dependent changes in H3K56ac in human cells.
JF  - Cell Cycle
VL  - 14
IS  - 24
SP  - 3851-3863
EP  - 3851-3863
PB  - Taylor&Francis Inc
SN  - 15384101
KW  - Cell cycle
KW  - Chromatin
KW  - DNA replication
KW  - H3K56ac
KW  - Mammalian cells
KW  - Nucleosome
UR  - http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760
L2  - http://www.tandfonline.com/doi/abs/10.1080/15384101.2015.1106760
N2  - The incorporation of histone H3 with an acetylated lysine 56 (H3K56ac) into the nucleosome is important for chromatin remodeling and serves as a marker of new nucleosomes during DNA replication and repair in yeast. However, in human cells, the level of H3K56ac is greatly reduced, and its role during the cell cycle is controversial. Our aim was to determine the potential of H3K56ac to regulate cell cycle progression in different human cell lines. A significant increase in the number of H3K56ac foci, but not in H3K56ac protein levels, was observed during the S and G2 phases in cancer cell lines, but was not observed in embryonic stem cell lines. Despite this increase, the H3K56ac signal was not present in late replication chromatin, and H3K56ac protein levels did not decrease after the inhibition of DNA replication. H3K56ac was not tightly associated with the chromatin and was primarily localized to active chromatin regions. Our results support the role of H3K56ac in transcriptionally active chromatin areas but do not confirm H3K56ac as a marker of newly synthetized nucleosomes in DNA replication.
ER  -

STEJSKAL, Stanislav, Karel ŠTĚPKA, Lenka TESAŘOVÁ, Karel STEJSKAL, Martina MATĚJKOVÁ, Pavel ŠIMARA, Zbyněk ZDRÁHAL a Irena KRONTORÁD KOUTNÁ. Cell cycle-dependent changes in H3K56ac in human cells. \textit{Cell Cycle}. Philadelphia: Taylor\&{}Francis Inc, 2015, roč.~14, č.~24, s.~3851-3863. ISSN~1538-4101. Dostupné z: https://dx.doi.org/10.1080/15384101.2015.1106760.

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