Molecular analysis of a bacterial chitinolytic community in an
upland pasture

J 2002

Molecular analysis of a bacterial chitinolytic community in an upland pasture

METCALFE, AC, Martin KRSEK, GW GOODAY, JI PROSSER, EMH WELLINGTON et. al.

Základní údaje

Originální název

Molecular analysis of a bacterial chitinolytic community in an upland pasture

Autoři

METCALFE, AC, Martin KRSEK, GW GOODAY, JI PROSSER a EMH WELLINGTON

Vydání

Applied and Environmental Microbiology, WASHINGTON, AMER SOC MICROBIOLOGY, 2002, 0099-2240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.691

DOI

http://dx.doi.org/10.1128/AEM.68.10.5042-5050.2002

UT WoS

000178380900046

Změněno: 8. 1. 2016 11:11, doc. Ing. Martin Krsek, CSc., MSc

Anotace

V originále

The effects of agricultural-improvement treatments on the chitinolytic activity and diversity of a microbial community were investigated within an upland pasture. The treatments of interest were lime and treated sewage sludge, both commonly applied to pasture land to improve fertility. Burial of chitin-containing litter bags at the field site resulted in enrichment of bacteria according to 16S rRNA fingerprinting. Chitinolytic-activity measurements showed that the highest activity occurred in those bags recovered from sludge-amended plots, which correlated well with increased counts of actinobacteria in samples from these chitin bags. Our findings suggest that sewage sludge increases the fertility of the soil in terms of chitinase activity. Ten clone libraries were constructed from family 18 subgroup A chitinases, PCR amplified from litter bags buried in soil in July 2000 or in September 2000, in a separate study. Analysis of these libraries by restriction fragment length polymorphism and sequencing showed that they were dominated by actinobacterium-like chitinase sequences. This suggests that actinobacteria have an important chitinolytic function in this soil ecosystem. Our findings showed that sludge application increased chitinolytic activity but decreased the diversity of chitinases present.

Citovat

METCALFE, AC, Martin KRSEK, GW GOODAY, JI PROSSER a EMH WELLINGTON. Molecular analysis of a bacterial chitinolytic community in an upland pasture. Applied and Environmental Microbiology. WASHINGTON: AMER SOC MICROBIOLOGY, 2002, roč. 68, č. 10, s. 5042-5050. ISSN 0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.68.10.5042-5050.2002.

@article{1323294,
   author = {Metcalfe, AC and Krsek, Martin and Gooday, GW and Prosser, JI and Wellington, EMH},
   article_location = {WASHINGTON},
   article_number = {10},
   doi = {http://dx.doi.org/10.1128/AEM.68.10.5042-5050.2002},
   language = {eng},
   issn = {0099-2240},
   journal = {Applied and Environmental Microbiology},
   title = {Molecular analysis of a bacterial chitinolytic community in an upland pasture},
   volume = {68},
   year = {2002}
}

TY  - JOUR
ID  - 1323294
AU  - Metcalfe, AC - Krsek, Martin - Gooday, GW - Prosser, JI - Wellington, EMH
PY  - 2002
TI  - Molecular analysis of a bacterial chitinolytic community in an upland pasture
JF  - Applied and Environmental Microbiology
VL  - 68
IS  - 10
SP  - 5042-5050
EP  - 5042-5050
PB  - AMER SOC MICROBIOLOGY
SN  - 00992240
N2  - The effects of agricultural-improvement treatments on the chitinolytic activity and diversity of a microbial community were investigated within an upland pasture. The treatments of interest were lime and treated sewage sludge, both commonly applied to pasture land to improve fertility. Burial of chitin-containing litter bags at the field site resulted in enrichment of bacteria according to 16S rRNA fingerprinting. Chitinolytic-activity measurements showed that the highest activity occurred in those bags recovered from sludge-amended plots, which correlated well with increased counts of actinobacteria in samples from these chitin bags. Our findings suggest that sewage sludge increases the fertility of the soil in terms of chitinase activity. Ten clone libraries were constructed from family 18 subgroup A chitinases, PCR amplified from litter bags buried in soil in July 2000 or in September 2000, in a separate study. Analysis of these libraries by restriction fragment length polymorphism and sequencing showed that they were dominated by actinobacterium-like chitinase sequences. This suggests that actinobacteria have an important chitinolytic function in this soil ecosystem. Our findings showed that sludge application increased chitinolytic activity but decreased the diversity of chitinases present.
ER  -

METCALFE, AC, Martin KRSEK, GW GOODAY, JI PROSSER a EMH WELLINGTON. Molecular analysis of a bacterial chitinolytic community in an upland pasture. \textit{Applied and Environmental Microbiology}. WASHINGTON: AMER SOC MICROBIOLOGY, 2002, roč.~68, č.~10, s.~5042-5050. ISSN~0099-2240. Dostupné z: https://dx.doi.org/10.1128/AEM.68.10.5042-5050.2002.

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