| KRSEK, Martin a EMH WELLINGTON. Assessment of chitin decomposer diversity within an upland grassland. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Dordrecht: Springer, 2001, roč. 79, 3-4, s. 261-267. ISSN 0003-6072. Dostupné z: https://dx.doi.org/10.1023/A:1012043401168. |
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@article{1323295,
author = {Krsek, Martin and Wellington, EMH},
article_location = {Dordrecht},
article_number = {3-4},
doi = {http://dx.doi.org/10.1023/A:1012043401168},
keywords = {actinomycetes; chitinase; DGGE; grassland ecology; litter bags; soil microcosms},
language = {eng},
issn = {0003-6072},
journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
title = {Assessment of chitin decomposer diversity within an upland grassland},
volume = {79},
year = {2001}
}
TY - JOUR
ID - 1323295
AU - Krsek, Martin - Wellington, EMH
PY - 2001
TI - Assessment of chitin decomposer diversity within an upland grassland
JF - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL - 79
IS - 3-4
SP - 261-267
EP - 261-267
PB - Springer
SN - 00036072
KW - actinomycetes
KW - chitinase
KW - DGGE
KW - grassland ecology
KW - litter bags
KW - soil microcosms
N2 - The breakdown of chitin within an acidic upland grassland was studied. The aim was to provide a molecular characterisation of microorganisms involved in chitin degradation in the soil using soil microcosms and buried litter bags containing chitin. The investigation involved an examination of the effects of liming on the microbial communities within the soil and their chitinolytic activity. Microcosm experiments were designed to study the influence of lime and chitin enrichment on the grassland soil bacterial community ex situ under controlled environmental conditions. Bacterial and actinomycete counts were determined and total community DNA was extracted from the microcosms and from chitin bags buried at the experimental site. PCR based on specific 16S rRNA target sequences provided products for DGGE analysis to determine the structure of bacterial and actinomycete communities. Chitinase activity was assessed spectrophotometrically using chitin labelled with remazol brilliant violet. Both liming and chitin amendment increased bacterial and actinomycete viable counts and the chitinase activity. DGGE band patterns confirmed changes in bacterial populations under the influence of both treatments. PCR products amplified from DNA isolated from chitin bags were cloned and sequenced. Only a few matched known species but a prominent coloniser of chitin proved to be Stenotrophomonas maltophilia.
ER -
KRSEK, Martin a EMH WELLINGTON. Assessment of chitin decomposer diversity within an upland grassland. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Dordrecht: Springer, 2001, roč.~79, 3-4, s.~261-267. ISSN~0003-6072. Dostupné z: https://dx.doi.org/10.1023/A:1012043401168.
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