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@proceedings{1324127,
author = {Fiala, Jan and Bittová, Miroslava and Havliš, Jan},
booktitle = {22th International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015)},
keywords = {Electrostatic repulsion hydrophilic interaction chromatography; trinucleotides; retention},
language = {eng},
title = {Retention behavior of trinucleotides in electrostatic repulsion hydrophilic interaction chromatography},
year = {2015}
}
TY - CONF
ID - 1324127
AU - Fiala, Jan - Bittová, Miroslava - Havliš, Jan
PY - 2015
TI - Retention behavior of trinucleotides in electrostatic repulsion hydrophilic interaction chromatography
KW - Electrostatic repulsion hydrophilic interaction chromatography
KW - trinucleotides
KW - retention
N2 - Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) was firstly introduced by Andrew J. Alpert in 2008 as an alternative chromatographic mode for separation of highly charged solutes such as peptides, amino acids and nucleotides. Combination of hydrophilic interactions and electrostatic attraction/repulsion in mentioned mixed mode chromatography offers efficient isocratic elution of solutes which would normally require gradient elution for their separation. Nowadays, the ERLIC mode is utilized mostly in proteomic research, nevertheless, its advantages call for the application in other biological fields, e.g. in polynucleotide analysis.
ER -
FIALA, Jan, Miroslava BITTOVÁ a Jan HAVLIŠ. Retention behavior of trinucleotides in electrostatic repulsion hydrophilic interaction chromatography. In \textit{22th International Symposium on Electro- and Liquid Phase-Separation Techniques (ITP2015)}. 2015.
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