Polymorphisms in the gene for vitamin D receptor in women with
gestational diabetes mellitus

a 2015

Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus

KURICOVÁ, Katarína, Anna PLESKAČOVÁ, Vendula BARTÁKOVÁ, Lukáš PÁCAL, Jitka ŘEHOŘOVÁ et. al.

Základní údaje

Originální název

Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus

Autoři

KURICOVÁ, Katarína, Anna PLESKAČOVÁ, Vendula BARTÁKOVÁ, Lukáš PÁCAL, Jitka ŘEHOŘOVÁ, Josef TOMANDL a Kateřina KAŇKOVÁ

Vydání

47th Annual Meeting of the Diabetic Pregnancy Study Group, Malaga, Spain, 2015

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Klíčová slova česky

gestační diabetes, SNPs, těhotenství, vitamín D, receptor VDR

Klíčová slova anglicky

gestational diabetes, SNPs, pregnancy, vitamin D, VDR receptor

Příznaky

Mezinárodní význam

Změněno: 13. 1. 2016 16:39, Mgr. Katarína Chalásová, Ph.D.

Anotace

V originále

Introduction: Vitamin D possesses a plethora of extraskeletal functions including regulation of cell proliferation, maturation and apoptosis, angiogenesis and is also affects insulin secretion and possibly insulin action. Via binding to vitamin D receptor (VDR) it regulates expression of hundreds of genes. A possible role of vitamin D in gestational diabetes (GDM) pathogenesis has been proposed. Several studies showed that vitamin D levels are lower in women with GDM compared to those with normal pregnancy. In our recent study we found that mid-gestational vitamin D levels did not significantly differ between pregnant women with GDM and healthy controls, while postpartum vitamin D levels raised significantly in both groups and in women with GDM history remained significantly lower compared to controls (Pleskáčová et al., 2015). Little is known about prevalence of VDR single nucleotide polymorphisms (SNP) and their possible relationship to plasma vitamin D levels in pregnant women with and without GDM. The aims of this study are to (i) compare allele and genotype frequencies of VDR SNPs between pregnant women with and without GDM and (ii) to analyze a relationship between VDR SNPs and vitamin D levels. Methods: Study comprised a total of 229 pregnant women with (n = 149) and without (n = 80) GDM. Genotyping for VDR single nucleotide polymorphisms (rs731236, rs7975232, rs1544410, rs2228570) was performed by real time PCR using TaqMan® assays. In a subgroup of women (47 with and 29 without GDM) we had two samples of vitamin D level: first, 24–30th week of gestation and second, 6 weeks–12 months after delivery. Results: Comparison of allele and genotype frequencies of studied SNPs did not show any significant difference between women with GDM and control group (P > 0.05, chi-square and Fisher-exact test). However, we found correlation between VDR SNP rs7975232 and vitamin D level postpartum in women with GDM (P = 0.0096, Kruskal-Wallis ANOVA). The AA genotype was associated with lower 25(OH)D level compared to other genotypes (P = 0.0066, Kruskal-Wallis ANOVA). Conclusion: In our previous study we confirmed overall high prevalence of vitamin D deficiency in pregnant women, without significant differences between GDM and control group. Although vitamin D levels in both groups were higher postpartum than during pregnancy, in women with previous GDM remained significantly lower than in control group – this was associated with AA genotype in VDR polymorphism (rs7975232).

Návaznosti

NT13198, projekt VaV

Název: Pentózový cyklus jako potenciální nový terapeutický cíl v prevenci diabetických komplikací

Investor: Ministerstvo zdravotnictví ČR, Pentózový cyklus jako potenciální nový terapeutický cíl v prevenci diabetických komplikací

Citovat

KURICOVÁ, Katarína, Anna PLESKAČOVÁ, Vendula BARTÁKOVÁ, Lukáš PÁCAL, Jitka ŘEHOŘOVÁ, Josef TOMANDL a Kateřina KAŇKOVÁ. Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus. In 47th Annual Meeting of the Diabetic Pregnancy Study Group, Malaga, Spain. 2015.

@proceedings{1324162,
   author = {Kuricová, Katarína and Pleskačová, Anna and Bartáková, Vendula and Pácal, Lukáš and Řehořová, Jitka and Tomandl, Josef and Kaňková, Kateřina},
   booktitle = {47th Annual Meeting of the Diabetic Pregnancy Study Group, Malaga, Spain},
   keywords = {gestational diabetes, SNPs, pregnancy, vitamin D, VDR receptor},
   language = {eng},
   title = {Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus},
   year = {2015}
}

TY  - CONF
ID  - 1324162
AU  - Kuricová, Katarína - Pleskačová, Anna - Bartáková, Vendula - Pácal, Lukáš - Řehořová, Jitka - Tomandl, Josef - Kaňková, Kateřina
PY  - 2015
TI  - Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus
KW  - gestational diabetes, SNPs, pregnancy, vitamin D, VDR receptor
N2  - Introduction: Vitamin D possesses a plethora of extraskeletal functions including regulation of cell proliferation, maturation and apoptosis, angiogenesis and is also affects insulin secretion and possibly insulin action. Via binding to vitamin D receptor (VDR) it regulates expression of hundreds of genes. A possible role of vitamin D in gestational diabetes (GDM) pathogenesis has been proposed. Several studies showed that vitamin D levels are lower in women with GDM compared to those with normal pregnancy. In our recent study we found that mid-gestational vitamin D levels did not significantly differ between pregnant women with GDM and healthy controls, while postpartum vitamin D levels raised significantly in both groups and in women with GDM history remained significantly lower compared to controls (Pleskáčová et al., 2015). Little is known about prevalence of VDR single nucleotide polymorphisms (SNP) and their possible relationship to plasma vitamin D levels in pregnant women with and without GDM. The aims of this study are to (i) compare allele and genotype frequencies of VDR SNPs between pregnant women with and without GDM and (ii) to analyze a relationship between VDR SNPs and vitamin D levels. Methods: Study comprised a total of 229 pregnant women with (n = 149) and without (n = 80) GDM. Genotyping for VDR single nucleotide polymorphisms (rs731236, rs7975232, rs1544410, rs2228570) was performed by real time PCR using TaqMan® assays. In a subgroup of women (47 with and 29 without GDM) we had two samples of vitamin D level: first, 24–30th week of gestation and second, 6 weeks–12 months after delivery. Results: Comparison of allele and genotype frequencies of studied SNPs did not show any significant difference between women with GDM and control group (P > 0.05, chi-square and Fisher-exact test). However, we found correlation between VDR SNP rs7975232 and vitamin D level postpartum in women with GDM (P = 0.0096, Kruskal-Wallis ANOVA). The AA genotype was associated with lower 25(OH)D level compared to other genotypes (P = 0.0066, Kruskal-Wallis ANOVA). Conclusion: In our previous study we confirmed overall high prevalence of vitamin D deficiency in pregnant women, without significant differences between GDM and control group. Although vitamin D levels in both groups were higher postpartum than during pregnancy, in women with previous GDM remained significantly lower than in control group – this was associated with AA genotype in VDR polymorphism (rs7975232).
ER  -

KURICOVÁ, Katarína, Anna PLESKAČOVÁ, Vendula BARTÁKOVÁ, Lukáš PÁCAL, Jitka ŘEHOŘOVÁ, Josef TOMANDL a Kateřina KAŇKOVÁ. Polymorphisms in the gene for vitamin D receptor in women with gestational diabetes mellitus. In \textit{47th Annual Meeting of the Diabetic Pregnancy Study Group, Malaga, Spain}. 2015.

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