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@proceedings{1324166,
author = {Mrázková, Jana and Malinovská, Lenka and Wimmerová, Michaela},
booktitle = {XVII. Setkání biochemiků a molekulárních biologů},
keywords = {lectins; RS-IIL; Ralstonia solanacearum; PA-IIL lectin superfamily; sugar-binding specificity change; site-directed mutagenesis; one point mutation},
language = {eng},
isbn = {978-80-210-8015-7},
title = {A point mutation led to overturn in sugar-binding specificity of lectin RS-IIL from PA-IIL lectin superfamily},
year = {2015}
}
TY - CONF
ID - 1324166
AU - Mrázková, Jana - Malinovská, Lenka - Wimmerová, Michaela
PY - 2015
TI - A point mutation led to overturn in sugar-binding specificity of lectin RS-IIL from PA-IIL lectin superfamily
SN - 9788021080157
KW - lectins
KW - RS-IIL
KW - Ralstonia solanacearum
KW - PA-IIL lectin superfamily
KW - sugar-binding specificity change
KW - site-directed mutagenesis
KW - one point mutation
N2 - The so-called “PA-IIL lectin superfamily” includes several lectins from human opportunistic pathogens: PA-IIL (from Pseudomonas aeruginosa), CV-IIL (from Chromobacterium violaceum), BC2L-A and C-terminal domain from BC2L-C (from Burkholderia cenocepacia). Also, it includes one lectin from important plant pathogen: RS-IIL (from Ralstonia solanacearum). All these lectins are homologues with significant sequential and structural similarities but they differ in sugar-binding specificity. This fact is caused by differences in three amino acids which are arranged in the so-called “specificity binding loop” (amino acids at positions 22-23-24). The previous study, which was focused on investigation of fine specificity of PA-IIL, confirmed that amino acid at position 22 is mainly responsible for sugar binding specificity. Specific amino acid substitution in this position led to change of sugar preference of PA-IIL to the RS-IIL one. In this work, we prepared a mutant of the RS-IIL lectin with substitution of amino acid at position 22 to the one present in PA-IIL “specificity loop” to test possibility of changing of sugar preference to the PA IIL one.
ER -
MRÁZKOVÁ, Jana, Lenka MALINOVSKÁ and Michaela WIMMEROVÁ. A point mutation led to overturn in sugar-binding specificity of lectin RS-IIL from PA-IIL lectin superfamily. In \textit{XVII. Setkání biochemiků a molekulárních biologů}. 2015. ISBN~978-80-210-8015-7.
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