Další formáty:
BibTeX
LaTeX
RIS
@article{1331166,
author = {Ptáčková, Nikola and Klempová, Jitka and Obořil, Michal and Nedělová, Sylvie and Lochman, Jan and Kašparovský, Tomáš},
article_location = {Paris},
article_number = {december},
doi = {http://dx.doi.org/10.1016/j.plaphy.2015.09.009},
keywords = {Cryptogein; Hydroxy fatty acids analysis; Hypersensitive response; Lipid peroxidation; Mutant proteins; Nicotiana tabaccum},
language = {eng},
issn = {0981-9428},
journal = {Plant Physiology and Biochemistry},
title = {The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum},
volume = {97},
year = {2015}
}
TY - JOUR
ID - 1331166
AU - Ptáčková, Nikola - Klempová, Jitka - Obořil, Michal - Nedělová, Sylvie - Lochman, Jan - Kašparovský, Tomáš
PY - 2015
TI - The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum
JF - Plant Physiology and Biochemistry
VL - 97
IS - december
SP - 82-95
EP - 82-95
PB - Elsevier
SN - 09819428
KW - Cryptogein
KW - Hydroxy fatty acids analysis
KW - Hypersensitive response
KW - Lipid peroxidation
KW - Mutant proteins
KW - Nicotiana tabaccum
N2 - Cryptogein, a protein from oomycete Phytophthora cryptogea, induces a hypersensitive cell death in Nicotiana tabacum. We prepared a new series of cryptogein mutant proteins with altered abilities to bind sterols and with altered charge distribution in the proteins. The effect of the mutations on the cryptogein ability to induce plant defence mechanisms associated with hypersensitive cell death were examined. Our results with new mutants support the previous findings that the sterol binding does not influence synthesis of ROS, cytosol acidification and development of leaf necrosis as these events seem to be more likely affected by the charge distribution and the overall protein structure. This hypothesis was also applicable on other mechanisms involved in the execution of plant cell death such as the NO generation, the stimulation of lipid peroxidation (determination of malondialdehyde and hydroxy fatty acids levels) and LOX gene transcription. In addition, the ability to bind sterols was found to serve not only for pathogen utilisation in its own metabolism but also to have an important function for the destabilization of plant membrane facilitating the pathogen spread inside the plant tissue as well as intensively contributing to the development of plant cell death. Considering the insertion of charged amino acid residues in the protein structure, the change localized in the protein surface affected its biological activity more effectively than that change inside the protein cavity. Moreover, the insertion of negative charged amino acids influenced mainly the events involved in the early phase of defence reaction, while the positive residues affected especially the necrotic activity of cryptogein.
ER -
PTÁČKOVÁ, Nikola, Jitka KLEMPOVÁ, Michal OBOŘIL, Sylvie NEDĚLOVÁ, Jan LOCHMAN a Tomáš KAŠPAROVSKÝ. The effect of cryptogein with changed abilities to transfer sterols and altered charge distribution on extracellular alkalinization, ROS and NO generation, lipid peroxidation and LOX gene transcription in Nicotiana tabacum. \textit{Plant Physiology and Biochemistry}. Paris: Elsevier, 2015, roč.~97, december, s.~82-95. ISSN~0981-9428. Dostupné z: https://dx.doi.org/10.1016/j.plaphy.2015.09.009.
|
