Different Doxorubicin Formulations Affect Plasma
4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde
Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

J 2015

Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ et. al.

Základní údaje

Originální název

Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat

Autoři

HLAVÁČOVÁ, Miroslava (703 Slovensko, domácí), Jaromír GUMULEC (203 Česká republika, domácí), Tibor STRAČINA (703 Slovensko, domácí), Michaela FOJTŮ (203 Česká republika, domácí), Martina RAUDENSKÁ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, domácí), Marie NOVÁKOVÁ (203 Česká republika, domácí) a Hana PAULOVÁ (203 Česká republika, garant, domácí)

Vydání

Physiological Research, Praha, Fyziologický ústav AV ČR, 2015, 0862-8408

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.643

Kód RIV

RIV/00216224:14110/15:00086241

Organizační jednotka

Lékařská fakulta

UT WoS

000372421900012

Klíčová slova anglicky

Doxorubicin; Drug nanotransporters; Oxidative stress; 4-hydroxy-2-nonenal; Thioredoxin reductase 2; Aldehyde dehydrogenase 3A1

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2016 13:22, Soňa Böhmová

Anotace

V originále

Increased oxidative stress is indisputably an important mechanism of doxorubicin side effects, especially its cardiotoxicity. To prevent Impairment of non-tumnrous tissue and to Improve the specificity in targeting the tumor tissue, new drug nanotransporters are developed. In many cases predinical therapeutic advantage has been shown when compared with the administration of conventional drug solution. Three forms of doxorubicin - conventional (DOX), encapsulated In liposomes (lipoDOX) and in apoferritin (apoDQX) were applied to Wistar rats. After 24 h exposition, the plasma level of 4-hydroxy-2-nonenal (4-HNE) as a marker of lipoperoxidalion and tissue gene expression of thioredoxln reductase 2 (TXNRDZ} and aldehyde dehydrogenase 3A1 (ALDH3AI) as an important part of antioxidative system were determined. Only conventional DOX significantly increases the level of 4-HNE; encapsulated forms on the other hand show significant decrease in plasma levels of 4-HNE In comparison with DOX. They also cause significant decrease In gene expression of ALDH3A1 and TXNRD2 In liver as a main detoxification organ, and a mild Influence on the expression of these enzymes in left heart ventride as a potential target of toxiclty. Thus, 4-HNE seems to be a good potential biomarker of oxidative stress induced by various forms of doxorubicin.

Návaznosti

MUNI/A/1195/2014, interní kód MU

Název: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Akronym: chebimo)

Investor: Masarykova univerzita, Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

MUNI/A/1326/2014, interní kód MU

Název: Kardiovaskulární systém od buňky k lůžku pacienta (Akronym: KASBUNPAC)

Investor: Masarykova univerzita, Kardiovaskulární systém od buňky k lůžku pacienta, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ, Michal MASAŘÍK, Marie NOVÁKOVÁ a Hana PAULOVÁ. Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat. Physiological Research. Praha: Fyziologický ústav AV ČR, 2015, roč. 64, Suppl. 5, s. "S653"-"S660", 8 s. ISSN 0862-8408.

@article{1332505,
   author = {Hlaváčová, Miroslava and Gumulec, Jaromír and Stračina, Tibor and Fojtů, Michaela and Raudenská, Martina and Masařík, Michal and Nováková, Marie and Paulová, Hana},
   article_location = {Praha},
   article_number = {Suppl. 5},
   keywords = {Doxorubicin; Drug nanotransporters; Oxidative stress; 4-hydroxy-2-nonenal; Thioredoxin reductase 2; Aldehyde dehydrogenase 3A1},
   language = {eng},
   issn = {0862-8408},
   journal = {Physiological Research},
   title = {Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat},
   volume = {64},
   year = {2015}
}

TY  - JOUR
ID  - 1332505
AU  - Hlaváčová, Miroslava - Gumulec, Jaromír - Stračina, Tibor - Fojtů, Michaela - Raudenská, Martina - Masařík, Michal - Nováková, Marie - Paulová, Hana
PY  - 2015
TI  - Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat
JF  - Physiological Research
VL  - 64
IS  - Suppl. 5
SP  - "S653"-"S660"
EP  - "S653"-"S660"
PB  - Fyziologický ústav AV ČR
SN  - 08628408
KW  - Doxorubicin
KW  - Drug nanotransporters
KW  - Oxidative stress
KW  - 4-hydroxy-2-nonenal
KW  - Thioredoxin reductase 2
KW  - Aldehyde dehydrogenase 3A1
N2  - Increased oxidative stress is indisputably an important mechanism of doxorubicin side effects, especially its cardiotoxicity. To prevent Impairment of non-tumnrous tissue and to Improve the specificity in targeting the tumor tissue, new drug nanotransporters are developed. In many cases predinical therapeutic advantage has been shown when compared with the administration of conventional drug solution. Three forms of doxorubicin - conventional (DOX), encapsulated In liposomes (lipoDOX) and in apoferritin (apoDQX) were applied to Wistar rats. After 24 h exposition, the plasma level of 4-hydroxy-2-nonenal (4-HNE) as a marker of lipoperoxidalion and tissue gene expression of thioredoxln reductase 2 (TXNRDZ} and aldehyde dehydrogenase 3A1 (ALDH3AI) as an important part of antioxidative system were determined. Only conventional DOX significantly increases the level of 4-HNE; encapsulated forms on the other hand show significant decrease in plasma levels of 4-HNE In comparison with DOX. They also cause significant decrease In gene expression of ALDH3A1 and TXNRD2 In liver as a main detoxification organ, and a mild Influence on the expression of these enzymes in left heart ventride as a potential target of toxiclty. Thus, 4-HNE seems to be a good potential biomarker of oxidative stress induced by various forms of doxorubicin.
ER  -

HLAVÁČOVÁ, Miroslava, Jaromír GUMULEC, Tibor STRAČINA, Michaela FOJTŮ, Martina RAUDENSKÁ, Michal MASAŘÍK, Marie NOVÁKOVÁ a Hana PAULOVÁ. Different Doxorubicin Formulations Affect Plasma 4-Hydroxy-2-Nonenal and Gene Expression of Aldehyde Dehydrogenase 3A1 and Thioredoxin Reductase 2 in Rat. \textit{Physiological Research}. Praha: Fyziologický ústav AV ČR, 2015, roč.~64, Suppl. 5, s.~''S653''-''S660'', 8 s. ISSN~0862-8408.

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