Identification of metabolites of selected benzophenanthridine
alkaloids and their toxicity evaluation

J 2016

Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation

SÁNDOR, Roman, Adam MIDLIK, Kristýna ŠEBRLOVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ et. al.

Basic information

Original name

Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation

Authors

SÁNDOR, Roman (703 Slovakia, belonging to the institution), Adam MIDLIK (703 Slovakia, belonging to the institution), Kristýna ŠEBRLOVÁ (203 Czech Republic, belonging to the institution), Gabriela DOVRTĚLOVÁ (203 Czech Republic, belonging to the institution), Kristýna NOSKOVÁ (203 Czech Republic, belonging to the institution), Jan JUŘICA (203 Czech Republic, belonging to the institution), Iva SLANINOVÁ (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Ondřej PEŠ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Pharmaceutical and Biomedical Analysis, Amsterdam, Elsevier Science BV, 2016, 0731-7085

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.255

RIV identification code

RIV/00216224:14110/16:00089320

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.jpba.2016.01.024

UT WoS

000371187100021

Keywords in English

benzophenanthridines; metabolites; rat liver microsomes; mass spectrometry; isolation

Abstract

V originále

Selected benzo[c]phenathridine alkaloids were biotransformed using rat liver microsomes and identified by liquid chromatography and mass spectrometry. While the metabolites of commercially available sanguinarine and chelerythrine have been studied in detail, data about the metabolism of the minor alkaloids remained unknown. Reactions involved in transformation include single and/or double O-demethylation, demethylenation, reduction, and hydroxylation. Two metabolites, when isolated, purified and tested for toxicity, were found to be less toxic than the original compounds.

Links

LH12176, research and development project

Name: Benzofenanthridinové alkaloidy - studium účinků na celulární a molekulární úrovni (Acronym: KBF)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1116/2014, interní kód MU

Name: Nové farmakologické mechanizmy v preklinickém i klinickém vývoji léčiv

Investor: Masaryk University, Category A

MUNI/A/1123/2014, interní kód MU

Name: Molekulární a buněčná biomedicína (Acronym: MBB)

Investor: Masaryk University, Category A

MUNI/A/1195/2014, interní kód MU

Name: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Acronym: chebimo)

Investor: Masaryk University, Category A

Citovat

SÁNDOR, Roman, Adam MIDLIK, Kristýna ŠEBRLOVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Iva SLANINOVÁ, Eva TÁBORSKÁ and Ondřej PEŠ. Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation. Journal of Pharmaceutical and Biomedical Analysis. Amsterdam: Elsevier Science BV, 2016, vol. 121, "neuvedeno", p. 174-180. ISSN 0731-7085. Available from: https://dx.doi.org/10.1016/j.jpba.2016.01.024.

@article{1333713,
   author = {Sándor, Roman and Midlik, Adam and Šebrlová, Kristýna and Dovrtělová, Gabriela and Nosková, Kristýna and Juřica, Jan and Slaninová, Iva and Táborská, Eva and Peš, Ondřej},
   article_location = {Amsterdam},
   article_number = {"neuvedeno"},
   doi = {http://dx.doi.org/10.1016/j.jpba.2016.01.024},
   keywords = {benzophenanthridines; metabolites; rat liver microsomes; mass spectrometry; isolation},
   language = {eng},
   issn = {0731-7085},
   journal = {Journal of Pharmaceutical and Biomedical Analysis},
   title = {Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation},
   url = {http://www.sciencedirect.com/science/article/pii/S0731708516300255},
   volume = {121},
   year = {2016}
}

TY  - JOUR
ID  - 1333713
AU  - Sándor, Roman - Midlik, Adam - Šebrlová, Kristýna - Dovrtělová, Gabriela - Nosková, Kristýna - Juřica, Jan - Slaninová, Iva - Táborská, Eva - Peš, Ondřej
PY  - 2016
TI  - Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation
JF  - Journal of Pharmaceutical and Biomedical Analysis
VL  - 121
IS  - "neuvedeno"
SP  - 174-180
EP  - 174-180
PB  - Elsevier Science BV
SN  - 07317085
KW  - benzophenanthridines
KW  - metabolites
KW  - rat liver microsomes
KW  - mass spectrometry
KW  - isolation
UR  - http://www.sciencedirect.com/science/article/pii/S0731708516300255
N2  - Selected benzo[c]phenathridine alkaloids were biotransformed using rat liver microsomes and identified by liquid chromatography and mass spectrometry. While the metabolites of commercially available sanguinarine and chelerythrine have been studied in detail, data about the metabolism of the minor alkaloids remained unknown. Reactions involved in transformation include single and/or double O-demethylation, demethylenation, reduction, and hydroxylation. Two metabolites, when isolated, purified and tested for toxicity, were found to be less toxic than the original compounds.
ER  -

SÁNDOR, Roman, Adam MIDLIK, Kristýna ŠEBRLOVÁ, Gabriela DOVRTĚLOVÁ, Kristýna NOSKOVÁ, Jan JUŘICA, Iva SLANINOVÁ, Eva TÁBORSKÁ and Ondřej PEŠ. Identification of metabolites of selected benzophenanthridine alkaloids and their toxicity evaluation. \textit{Journal of Pharmaceutical and Biomedical Analysis}. Amsterdam: Elsevier Science BV, 2016, vol.~121, ''neuvedeno'', p.~174-180. ISSN~0731-7085. Available from: https://dx.doi.org/10.1016/j.jpba.2016.01.024.

Detailed Information on Publication Record