On-line Coupling of Immobilized Cytochrome P450 Microreactor
and Capillary Electrophoresis: A Promising Tool for Drug
Development

J 2016

On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development

SCHEJBAL, Jan, Roman ŘEMÍNEK, Lukáš ZEMAN, Aleš MÁDR, Zdeněk GLATZ et. al.

Basic information

Original name

On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development

Name in Czech

On-line spojení enzymového reaktoru s cytochromem P450 a kapilární elektroforézou, slibný nástroj pro vývoj nových léčiv

Authors

SCHEJBAL, Jan (203 Czech Republic, belonging to the institution), Roman ŘEMÍNEK (203 Czech Republic, belonging to the institution), Lukáš ZEMAN (203 Czech Republic, belonging to the institution), Aleš MÁDR (203 Czech Republic, belonging to the institution) and Zdeněk GLATZ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Chromatography A, Amsterdam, Netherlands, Elsevier, 2016, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.981

RIV identification code

RIV/00216224:14310/16:00087795

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.chroma.2016.01.081

UT WoS

000371556700026

Keywords (in Czech)

kapilární elektroforéza; enzymový reaktor; cytochromy P450; vývoj nových léčiv

Keywords in English

capillary electrophoresis; IMER; IMER; Cytochromes P450; drug developemnt

Abstract

ORIG CZ

V originále

In this work, the combination of an immobilized enzyme microreactor (IMER) based on the clinically important isoform cytochrome P450 2C9 (CYP2C9) with capillary electrophoresis (CE) is presented. The CYP2C9 was attached to magnetic SiMAG-carboxyl microparticles using the carbodiimide method. The formation of an IMER in the inlet part of the separation capillary was ensured by two permanent magnets fixed in a cassette from the CE apparatus in the repulsive arrangement. The resulting on-line system provides an integration of enzyme reaction mixing and incubation, reaction products separation, detection and quantification into a single fully automated procedure with the possibility of repetitive use of the enzyme and minuscule amounts of reactant consumption. The on-line kinetic and inhibition studies of CYP2C9’s reaction with diclofenac as a model substrate and sulfaphenazole as a model inhibitor were conducted in order to demonstrate its practical applicability.

In Czech

Byl zkonstruován enzymový reaktor na bázi cytochromy P450 izoformy CYP2C9 a spojen s kapilární elektroforézou pro studium metabolismu léčiv.

Links

GBP206/12/G014, research and development project

Name: Centrum pokročilých bioanalytických technologií

MUNI/A/1265/2015, interní kód MU

Name: Podpora biochemického výzkumu v roce 2016

Investor: Masaryk University, Category A

Citovat

SCHEJBAL, Jan, Roman ŘEMÍNEK, Lukáš ZEMAN, Aleš MÁDR and Zdeněk GLATZ. On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development. Journal of Chromatography A. Amsterdam, Netherlands: Elsevier, 2016, vol. 1437, March, p. 234-240. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2016.01.081.

@article{1333753,
   author = {Schejbal, Jan and Řemínek, Roman and Zeman, Lukáš and Mádr, Aleš and Glatz, Zdeněk},
   article_location = {Amsterdam, Netherlands},
   article_number = {March},
   doi = {http://dx.doi.org/10.1016/j.chroma.2016.01.081},
   keywords = {capillary electrophoresis; IMER; IMER; Cytochromes P450; drug developemnt},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development},
   volume = {1437},
   year = {2016}
}

TY  - JOUR
ID  - 1333753
AU  - Schejbal, Jan - Řemínek, Roman - Zeman, Lukáš - Mádr, Aleš - Glatz, Zdeněk
PY  - 2016
TI  - On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development
JF  - Journal of Chromatography A
VL  - 1437
IS  - March
SP  - 234-240
EP  - 234-240
PB  - Elsevier
SN  - 00219673
KW  - capillary electrophoresis
KW  - IMER
KW  - IMER
KW  - Cytochromes P450
KW  - drug developemnt
N2  - In this work, the combination of an immobilized enzyme microreactor (IMER) based on the clinically important isoform cytochrome P450 2C9 (CYP2C9) with capillary electrophoresis (CE) is presented. The CYP2C9 was attached to magnetic SiMAG-carboxyl microparticles using the carbodiimide method. The formation of an IMER in the inlet part of the separation capillary was ensured by two permanent magnets fixed in a cassette from the CE apparatus in the repulsive arrangement. The resulting on-line system provides an integration of enzyme reaction mixing and incubation, reaction products separation, detection and quantification into a single fully automated procedure with the possibility of repetitive use of the enzyme and minuscule amounts of reactant consumption. The on-line kinetic and inhibition studies of CYP2C9’s reaction with diclofenac as a model substrate and sulfaphenazole as a model inhibitor were conducted in order to demonstrate its practical applicability.
ER  -

SCHEJBAL, Jan, Roman ŘEMÍNEK, Lukáš ZEMAN, Aleš MÁDR and Zdeněk GLATZ. On-line Coupling of Immobilized Cytochrome P450 Microreactor and Capillary Electrophoresis: A Promising Tool for Drug Development. \textit{Journal of Chromatography A}. Amsterdam, Netherlands: Elsevier, 2016, vol.~1437, March, p.~234-240. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2016.01.081.

Detailed Information on Publication Record