Direct communication of the spinal subarachnoid space with the
rat dorsal root ganglia

J 2016

Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia

JOUKAL, Marek, Ilona KLUSÁKOVÁ a Petr DUBOVÝ

Základní údaje

Originální název

Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia

Autoři

JOUKAL, Marek (203 Česká republika, garant, domácí), Ilona KLUSÁKOVÁ (203 Česká republika, domácí) a Petr DUBOVÝ (203 Česká republika, domácí)

Vydání

Annals of Anatomy - Anatomischer Anzeiger, Jena, Elsevier, 2016, 0940-9602

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.864

Kód RIV

RIV/00216224:14110/16:00089382

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.aanat.2016.01.004

UT WoS

000376548200002

Klíčová slova anglicky

Subarachnoid space; Cerebrospinal fluid; Dorsal root ganglia; Fluoro-emerald; Immunohistochemistry; Macrophages

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2017 10:18, Soňa Böhmová

Anotace

V originále

The anatomical position of the subarachnoid space (SAS) in relation to dorsal root ganglia (DRG) and penetration of tracer from the SAS into DRG were investigated. We used intrathecal injection of methylene blue to visualize the anatomical position of the SAS in relation to DRG and immunostaining of dipeptidyl peptidase IV (DPP-IV) for detecting arachnoid limiting the SAS. Intrathecal administration of fluorescent-conjugated dextran (fluoro-emerald; FE) was used to demonstrate direct communication between the SAS and DRG. Intrathecal injection of methylene blue and DPP-IV immunostaining revealed that SAS delimited by the arachnoid was extended up to the capsule of DRG in a fold-like recess that may reach approximately half of the DRG length. The arachnoid was found in direct contact to the neuronal body-rich area in the angle between dorsal root and DRG as well as between spinal nerve roots at DRG. Particles of FE were found in the cells of DRG capsule, satellite glial cells, interstitial space, as well as in small and medium-sized neurons after intrathecal injection. Penetration of FE from the SAS into the DRG induced an immune reaction expressed by colocalization of FE and immunofluorescence indicating antigen-presenting cells (MHC-II+), activated (ED1+) and resident (ED2+) macrophages, and activation of satellite glial cells (GFAP+). Penetration of lumbar-injected FE into the cervical DRG was greater than that into the lumbar DRG after intrathecal injection of FE into the cisterna magna. Our results demonstrate direct communication between DRG and cerebrospinal fluid in the SAS that can create another pathway for possible propagation of inflammatory and signaling molecules from DRG primary affected by peripheral nerve injury into DRG of remote spinal segments.

Návaznosti

MUNI/A/1215/2014, interní kód MU

Název: Studium hemato-likvorové bariéry laboratorního potkana po poškození periferního nervu

Investor: Masarykova univerzita, Studium hemato-likvorové bariéry laboratorního potkana po poškození periferního nervu, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

JOUKAL, Marek, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia. Annals of Anatomy - Anatomischer Anzeiger. Jena: Elsevier, 2016, roč. 205, 1 May 2016, s. 9-15. ISSN 0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2016.01.004.

@article{1335227,
   author = {Joukal, Marek and Klusáková, Ilona and Dubový, Petr},
   article_location = {Jena},
   article_number = {1 May 2016},
   doi = {http://dx.doi.org/10.1016/j.aanat.2016.01.004},
   keywords = {Subarachnoid space; Cerebrospinal fluid; Dorsal root ganglia; Fluoro-emerald; Immunohistochemistry; Macrophages},
   language = {eng},
   issn = {0940-9602},
   journal = {Annals of Anatomy - Anatomischer Anzeiger},
   title = {Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia},
   volume = {205},
   year = {2016}
}

TY  - JOUR
ID  - 1335227
AU  - Joukal, Marek - Klusáková, Ilona - Dubový, Petr
PY  - 2016
TI  - Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia
JF  - Annals of Anatomy - Anatomischer Anzeiger
VL  - 205
IS  - 1 May 2016
SP  - 9-15
EP  - 9-15
PB  - Elsevier
SN  - 09409602
KW  - Subarachnoid space
KW  - Cerebrospinal fluid
KW  - Dorsal root ganglia
KW  - Fluoro-emerald
KW  - Immunohistochemistry
KW  - Macrophages
N2  - The anatomical position of the subarachnoid space (SAS) in relation to dorsal root ganglia (DRG) and penetration of tracer from the SAS into DRG were investigated. We used intrathecal injection of methylene blue to visualize the anatomical position of the SAS in relation to DRG and immunostaining of dipeptidyl peptidase IV (DPP-IV) for detecting arachnoid limiting the SAS. Intrathecal administration of fluorescent-conjugated dextran (fluoro-emerald; FE) was used to demonstrate direct communication between the SAS and DRG. Intrathecal injection of methylene blue and DPP-IV immunostaining revealed that SAS delimited by the arachnoid was extended up to the capsule of DRG in a fold-like recess that may reach approximately half of the DRG length. The arachnoid was found in direct contact to the neuronal body-rich area in the angle between dorsal root and DRG as well as between spinal nerve roots at DRG. Particles of FE were found in the cells of DRG capsule, satellite glial cells, interstitial space, as well as in small and medium-sized neurons after intrathecal injection. Penetration of FE from the SAS into the DRG induced an immune reaction expressed by colocalization of FE and immunofluorescence indicating antigen-presenting cells (MHC-II+), activated (ED1+) and resident (ED2+) macrophages, and activation of satellite glial cells (GFAP+). Penetration of lumbar-injected FE into the cervical DRG was greater than that into the lumbar DRG after intrathecal injection of FE into the cisterna magna. Our results demonstrate direct communication between DRG and cerebrospinal fluid in the SAS that can create another pathway for possible propagation of inflammatory and signaling molecules from DRG primary affected by peripheral nerve injury into DRG of remote spinal segments.
ER  -

JOUKAL, Marek, Ilona KLUSÁKOVÁ a Petr DUBOVÝ. Direct communication of the spinal subarachnoid space with the rat dorsal root ganglia. \textit{Annals of Anatomy - Anatomischer Anzeiger}. Jena: Elsevier, 2016, roč.~205, 1 May 2016, s.~9-15. ISSN~0940-9602. Dostupné z: https://dx.doi.org/10.1016/j.aanat.2016.01.004.

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