Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded
from cyprinids from the luvuvvhu River. Morhpological analysis
and molecular characterisation.

a 2015

Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation.

PŘIKRYLOVÁ, Iva, Šárka MAŠOVÁ, Moses M. MATLA, Milan GELNAR, Sareh TAVAKOL et. al.

Basic information

Original name

Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation.

Authors

PŘIKRYLOVÁ, Iva (203 Czech Republic, guarantor, belonging to the institution), Šárka MAŠOVÁ (203 Czech Republic), Moses M. MATLA (710 South Africa), Milan GELNAR (203 Czech Republic), Sareh TAVAKOL (364 Islamic Republic of Iran) and Wilmien J. LUUS-POWELL (710 South Africa)

Edition

44th Annual Confrences of the Parasitological Society of Southern Africa. 2015

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

South Africa

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/15:00081593

Organization unit

Faculty of Science

Keywords in English

Afrodiplozoon; SEM; morphometrics; ITS rDNA; Diplozoidae

Změněno: 18/2/2016 09:45, Mgr. Iva Přikrylová, Ph.D.

Abstract

V originále

he genus Afrodiplozoon was proposed by Khotenovsky in 1981 when Afrodiplozoon polycotyleus (Paperna, 1963) has been exluded from the genus Neodiplozoon Tripathi, 1959 based on the number of clamps on the attachment apparatus. Since then, the inconsistency in the use of the name for this parasite can be seen in the literature. Specimens of A. polycotyleus were collected during a fish survey carried out in April and July 2014 in the Venda region, Limpopo Province, South Africa, from Labeobarbus maraquensis (n=36; mean total length=7.9 cm) and Barbus paludinosus (n=1; total length=6.6 cm). A prevalences of 64% and 59% were recorded for A. polycotyleus during April and July, respectively. Morphological analysis of the composition of the internal organs and attachment clamps using different microscopic methods (light microscopy of stained/unstained specimens and scanning electron microscopy, SEM) provided details for the redescription of the genus. The type material was also studied. Molecular characterization based on the variabily of the second internal transcribed spacer (ITS2) rDNA showed the taxonomic relationship to other representatives of Diplozoidae. Parasites can bear asymetrically from 7 up to 10 clamps in one row on each side of the attachment apparatus, with the first clamp is significantly smaller. The connection sclerite of posterior end of the central plate is wide in all its length. The anterior end of the central plate has short processes. The SEM observations showed the presence of numerous papilae around the mouth of the worm. Molecular characterization and subsequent analysis revealed as a sister species to A. polycotyleus recently described Paradiplozoon bingolensis (Civáňová, Koyun, Koubková, 2013) both positioned far from African representatives of the genus Paradiplozoon (Achmerov, 1974).

Links

GBP505/12/G112, research and development project

Name: ECIP - Evropské centrum ichtyoparazitologie

Investor: Czech Science Foundation

Citovat

PŘIKRYLOVÁ, Iva, Šárka MAŠOVÁ, Moses M. MATLA, Milan GELNAR, Sareh TAVAKOL and Wilmien J. LUUS-POWELL. Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation. In 44th Annual Confrences of the Parasitological Society of Southern Africa. 2015.

@proceedings{1336191,
   author = {Přikrylová, Iva and Mašová, Šárka and Matla, Moses M. and Gelnar, Milan and Tavakol, Sareh and LuusandPowell, Wilmien J.},
   booktitle = {44th Annual Confrences of the Parasitological Society of Southern Africa.},
   keywords = {Afrodiplozoon; SEM; morphometrics; ITS rDNA; Diplozoidae},
   language = {eng},
   title = {Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation.},
   year = {2015}
}

TY  - CONF
ID  - 1336191
AU  - Přikrylová, Iva - Mašová, Šárka - Matla, Moses M. - Gelnar, Milan - Tavakol, Sareh - Luus-Powell, Wilmien J.
PY  - 2015
TI  - Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation.
KW  - Afrodiplozoon
KW  - SEM
KW  - morphometrics
KW  - ITS rDNA
KW  - Diplozoidae
N2  - he genus Afrodiplozoon was proposed by Khotenovsky in 1981 when Afrodiplozoon polycotyleus (Paperna, 1963) has been exluded from the genus Neodiplozoon Tripathi, 1959 based on the number of clamps on the attachment apparatus. Since then, the inconsistency in the use of the name for this parasite can be seen in the literature. Specimens of A. polycotyleus were collected during a fish survey carried out in April and July 2014 in the Venda region, Limpopo Province, South Africa, from Labeobarbus maraquensis (n=36; mean total length=7.9 cm) and Barbus paludinosus (n=1; total length=6.6 cm). A prevalences of 64% and 59% were recorded for A. polycotyleus during April and July, respectively. Morphological analysis of the composition of the internal organs and attachment clamps using different microscopic methods (light microscopy of stained/unstained specimens and scanning electron microscopy, SEM) provided details for the redescription of the genus. The type material was also studied. Molecular characterization based on the variabily of the second internal transcribed spacer (ITS2) rDNA showed the taxonomic relationship to other representatives of Diplozoidae. Parasites can bear asymetrically from 7 up to 10 clamps in one row on each side of the attachment apparatus, with the first clamp is significantly smaller. The connection sclerite of posterior end of the central plate is wide in all its length. The anterior end of the central plate has short processes. The SEM observations showed the presence of numerous papilae around the mouth of the worm. Molecular characterization and subsequent analysis revealed as a sister species to A. polycotyleus recently described Paradiplozoon bingolensis (Civáňová, Koyun, Koubková, 2013) both positioned far from African representatives of the genus Paradiplozoon (Achmerov, 1974).
ER  -

PŘIKRYLOVÁ, Iva, Šárka MAŠOVÁ, Moses M. MATLA, Milan GELNAR, Sareh TAVAKOL and Wilmien J. LUUS-POWELL. Afrodiplozoon polycotyleus (Monogenea: Diplozoidae) recorded from cyprinids from the luvuvvhu River. Morhpological analysis and molecular characterisation. In \textit{44th Annual Confrences of the Parasitological Society of Southern Africa.}. 2015.

Detailed Information on Publication Record