Retinoid compounds associated with water blooms dominated by
Microcystis species

J 2015

Retinoid compounds associated with water blooms dominated by Microcystis species

JAVŮREK, Jakub, Eliška SYCHROVÁ, Marie SMUTNÁ, Michal BITTNER, Jiří KOHOUTEK et. al.

Základní údaje

Originální název

Retinoid compounds associated with water blooms dominated by Microcystis species

Autoři

JAVŮREK, Jakub (203 Česká republika, domácí), Eliška SYCHROVÁ (203 Česká republika, domácí), Marie SMUTNÁ (203 Česká republika, domácí), Michal BITTNER (203 Česká republika, domácí), Jiří KOHOUTEK (203 Česká republika, domácí), Ondřej ADAMOVSKÝ (203 Česká republika, domácí), Kateřina NOVÁKOVÁ (203 Česká republika, domácí), Soňa SMETANOVÁ (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Harmful Algae, AMSTERDAM (NETHERLANDS), Elsevier Science, 2015, 1568-9883

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.664

Kód RIV

RIV/00216224:14310/15:00081599

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.hal.2015.06.006

UT WoS

000359027400012

Klíčová slova anglicky

Retinoic acid receptor; Cyanobacteria; Biomass extracts; All-trans-retinoic acid; 9-cis Retinoic acid; Retinoid-like activity

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 7. 2017 13:23, RNDr. Mgr. Michal Bittner, Ph.D.

Anotace

V originále

Retinoic acids play a critical role in vital physiological processes and vertebrate development, their derivatives can be produced by some cyanobacterial species into surface waters. This study presents environmentally-relevant information on total retinoid-like activity of field cyanobacterial biomasses and surrounding waters. Intracellular and extracellular levels of total retinoid-like activity and retinoic acids have been investigated at a set of independent sites with the occurrence of water bloom species Microcystis aeruginosa. Twelve samples of biomass and surrounding water from seven localities affected by blooms were studied in comparison with samples from M. aeruginosa laboratory cultures. The method for biomass extraction was optimized and final extracts and samples of surrounding water concentrated by solid phase extraction were assessed using in vitro reporter gene bioassay and chemical analyses for all-trans-retinoic acid (ATRA), 9-cis retinoic acid (9-cis RA) and microcystins RR, LR and YR. Methanol was the most efficient solvent for the extraction of compounds with retinoid-like activity. An in vitro bioassay with the P19/A15 transgenic cell line revealed retinoid-like activity in all cyanobacterial biomasses in the range of 356-2838 ng of retinoid acid equivalents (REQ)/g dry mass (dm), while only three of surrounding water samples exhibited detectable retinoid-like activity, in the range of 12.8-28.7 ng REQ/L. Microcystins were detected in all samples, but they elicited no detectable retinoid-like activity up to 10 mg/L. Chemical analyses detected concentrations up to 340 ng/g dm of all-trans-retinoic acid (ATRA) and 84 ng/g dm 9-cis retinoic acid (9-cis RA) in bloom extracts, and up to 19 ng/L ATRA and 2.2 ng/L 9-cis RA in surrounding water. In most samples, ATRA and 9-cis RA contributed relatively little to the total REQs, which indicates the presence of significant amounts of other compounds with retinoic acid receptor-mediated modes of action.

Návaznosti

GAP503/12/0553, projekt VaV

Název: Metabolity sinic jako potenciální endokrinní disruptory

Investor: Grantová agentura ČR, Metabolity sinic jako potenciální endokrinní disruptory

LM2011028, projekt VaV

Název: RECETOX ? Národní infrastruktura pro výzkum toxických látek v prostředí

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, RECETOX Národní infrastruktura pro výzkum toxických látek v prostředí

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

JAVŮREK, Jakub, Eliška SYCHROVÁ, Marie SMUTNÁ, Michal BITTNER, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, Kateřina NOVÁKOVÁ, Soňa SMETANOVÁ a Klára HILSCHEROVÁ. Retinoid compounds associated with water blooms dominated by Microcystis species. Harmful Algae. AMSTERDAM (NETHERLANDS): Elsevier Science, 2015, roč. 47, JULY, s. 116-125. ISSN 1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2015.06.006.

@article{1336228,
   author = {Javůrek, Jakub and Sychrová, Eliška and Smutná, Marie and Bittner, Michal and Kohoutek, Jiří and Adamovský, Ondřej and Nováková, Kateřina and Smetanová, Soňa and Hilscherová, Klára},
   article_location = {AMSTERDAM (NETHERLANDS)},
   article_number = {JULY},
   doi = {http://dx.doi.org/10.1016/j.hal.2015.06.006},
   keywords = {Retinoic acid receptor; Cyanobacteria; Biomass extracts; All-trans-retinoic acid; 9-cis Retinoic acid; Retinoid-like activity},
   language = {eng},
   issn = {1568-9883},
   journal = {Harmful Algae},
   title = {Retinoid compounds associated with water blooms dominated by Microcystis species},
   url = {http://www.sciencedirect.com/science/article/pii/S1568988315000992},
   volume = {47},
   year = {2015}
}

TY  - JOUR
ID  - 1336228
AU  - Javůrek, Jakub - Sychrová, Eliška - Smutná, Marie - Bittner, Michal - Kohoutek, Jiří - Adamovský, Ondřej - Nováková, Kateřina - Smetanová, Soňa - Hilscherová, Klára
PY  - 2015
TI  - Retinoid compounds associated with water blooms dominated by Microcystis species
JF  - Harmful Algae
VL  - 47
IS  - JULY
SP  - 116-125
EP  - 116-125
PB  - Elsevier Science
SN  - 15689883
KW  - Retinoic acid receptor
KW  - Cyanobacteria
KW  - Biomass extracts
KW  - All-trans-retinoic acid
KW  - 9-cis Retinoic acid
KW  - Retinoid-like activity
UR  - http://www.sciencedirect.com/science/article/pii/S1568988315000992
L2  - http://www.sciencedirect.com/science/article/pii/S1568988315000992
N2  - Retinoic acids play a critical role in vital physiological processes and vertebrate development, their derivatives can be produced by some cyanobacterial species into surface waters. This study presents environmentally-relevant information on total retinoid-like activity of field cyanobacterial biomasses and surrounding waters. Intracellular and extracellular levels of total retinoid-like activity and retinoic acids have been investigated at a set of independent sites with the occurrence of water bloom species Microcystis aeruginosa. Twelve samples of biomass and surrounding water from seven localities affected by blooms were studied in comparison with samples from M. aeruginosa laboratory cultures. The method for biomass extraction was optimized and final extracts and samples of surrounding water concentrated by solid phase extraction were assessed using in vitro reporter gene bioassay and chemical analyses for all-trans-retinoic acid (ATRA), 9-cis retinoic acid (9-cis RA) and microcystins RR, LR and YR. Methanol was the most efficient solvent for the extraction of compounds with retinoid-like activity. An in vitro bioassay with the P19/A15 transgenic cell line revealed retinoid-like activity in all cyanobacterial biomasses in the range of 356-2838 ng of retinoid acid equivalents (REQ)/g dry mass (dm), while only three of surrounding water samples exhibited detectable retinoid-like activity, in the range of 12.8-28.7 ng REQ/L. Microcystins were detected in all samples, but they elicited no detectable retinoid-like activity up to 10 mg/L. Chemical analyses detected concentrations up to 340 ng/g dm of all-trans-retinoic acid (ATRA) and 84 ng/g dm 9-cis retinoic acid (9-cis RA) in bloom extracts, and up to 19 ng/L ATRA and 2.2 ng/L 9-cis RA in surrounding water. In most samples, ATRA and 9-cis RA contributed relatively little to the total REQs, which indicates the presence of significant amounts of other compounds with retinoic acid receptor-mediated modes of action.
ER  -

JAVŮREK, Jakub, Eliška SYCHROVÁ, Marie SMUTNÁ, Michal BITTNER, Jiří KOHOUTEK, Ondřej ADAMOVSKÝ, Kateřina NOVÁKOVÁ, Soňa SMETANOVÁ a Klára HILSCHEROVÁ. Retinoid compounds associated with water blooms dominated by Microcystis species. \textit{Harmful Algae}. AMSTERDAM (NETHERLANDS): Elsevier Science, 2015, roč.~47, JULY, s.~116-125. ISSN~1568-9883. Dostupné z: https://dx.doi.org/10.1016/j.hal.2015.06.006.

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