| FETTER, Eva, Soňa SMETANOVÁ, Lisa BALDAUF, Annegret LIDZBA, Rolf ALTENBURGER, Andreas SCHUTTLER a Stefan SCHOLZ. Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos. Environmental Science and Technology. American Chemical Society, 2015, roč. 49, č. 19, s. 11789-11798. ISSN 0013-936X. Dostupné z: https://dx.doi.org/10.1021/acs.est.5b01034. |
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@article{1336263,
author = {Fetter, Eva and Smetanová, Soňa and Baldauf, Lisa and Lidzba, Annegret and Altenburger, Rolf and Schuttler, Andreas and Scholz, Stefan},
article_number = {19},
doi = {http://dx.doi.org/10.1021/acs.est.5b01034},
keywords = {DISRUPTING CHEMICALS; DANIO-RERIO; FUNCTIONAL-CHARACTERIZATION; ENDOCRINE DISRUPTION; MODEL SELECTION; RAINBOW-TROUT; MESSENGER-RNA; THYROID-GLAND; EXPRESSION; RECEPTOR},
language = {eng},
issn = {0013-936X},
journal = {Environmental Science and Technology},
title = {Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos},
url = {http://pubs.acs.org/doi/10.1021/acs.est.5b01034},
volume = {49},
year = {2015}
}
TY - JOUR
ID - 1336263
AU - Fetter, Eva - Smetanová, Soňa - Baldauf, Lisa - Lidzba, Annegret - Altenburger, Rolf - Schuttler, Andreas - Scholz, Stefan
PY - 2015
TI - Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos
JF - Environmental Science and Technology
VL - 49
IS - 19
SP - 11789-11798
EP - 11789-11798
PB - American Chemical Society
SN - 0013936X
KW - DISRUPTING CHEMICALS
KW - DANIO-RERIO
KW - FUNCTIONAL-CHARACTERIZATION
KW - ENDOCRINE DISRUPTION
KW - MODEL SELECTION
KW - RAINBOW-TROUT
KW - MESSENGER-RNA
KW - THYROID-GLAND
KW - EXPRESSION
KW - RECEPTOR
UR - http://pubs.acs.org/doi/10.1021/acs.est.5b01034
L2 - http://pubs.acs.org/doi/10.1021/acs.est.5b01034
N2 - Responsive genes for fish embryos have been identified so far for some endocrine pathways but not for androgens. Using transcriptome analysis and multiple concentration response modeling, we identified putative androgen-responsive genes in zebrafish embryos exposed to 0.05-5000 nM 11-ketotestosterone for 24 h. Four selected genes with sigmoidal concentration-dependent expression profiles (EC50 = 6.5-30.0 nM) were characterized in detail. The expression of cyp2k22 and slco1f4 was demonstrated in the pronephros; lipca was detected in the liver, and sult2st3 was found in the olfactory organs and choroid plexus. Their expression domains, the function of human orthologs, and a pathway analysis suggested a role of these genes in the metabolism of hormones. Hence, it was hypothesized that they were induced to compensate for elevated hormone levels. The induction of sult2st3 and cyp2k22 by 11-ketotestosterone was repressed by co-exposure to the androgen receptor antagonist nilutamide supporting a potential androgen receptor mediated regulation. Sensitivity (expressed as EC50 values) of sult2st3 and cyp2k22 gene expression induction after exposure to other steroidal hormones (11-ketotestosterone similar to testosterone > progesterone > cortisol > ethinylestradiol) correlated with their known binding affinities to zebrafish androgen receptor. Hence, these genes might represent potential markers for screening of androgenic compounds in the zebrafish embryo.
ER -
FETTER, Eva, Soňa SMETANOVÁ, Lisa BALDAUF, Annegret LIDZBA, Rolf ALTENBURGER, Andreas SCHUTTLER a Stefan SCHOLZ. Identification and Characterization of Androgen-Responsive Genes in Zebrafish Embryos. \textit{Environmental Science and Technology}. American Chemical Society, 2015, roč.~49, č.~19, s.~11789-11798. ISSN~0013-936X. Dostupné z: https://dx.doi.org/10.1021/acs.est.5b01034.
|
