The structure and DNA-binding properties of Mgm101 from a yeast
with a linear mitochondrial genome

J 2016

The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome

PEVALA, Vladimír, Dominika TRUBAN, Jacob A. BAUER, Július KOŠŤAN, Nina KUNOVÁ et. al.

Základní údaje

Originální název

The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome

Autoři

PEVALA, Vladimír (703 Slovensko), Dominika TRUBAN (703 Slovensko), Jacob A. BAUER (703 Slovensko), Július KOŠŤAN (40 Rakousko), Nina KUNOVÁ (703 Slovensko), Jana BELLOVÁ (703 Slovensko), Marlene BRANDSTETTER (40 Rakousko), María Victoria MARINI PALOMEQUE (858 Uruguay, domácí), Lumír KREJČÍ (203 Česká republika, garant, domácí), Ľubomír TOMÁŠKA (703 Slovensko), Jozef NOSEK (703 Slovensko) a Eva KUTEJOVÁ (703 Slovensko)

Vydání

Nucleic Acids Research, Oxford, Oxford University Press, 2016, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 10.162

Kód RIV

RIV/00216224:14110/16:00087823

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1093/nar/gkv1529

UT WoS

000373723100031

Klíčová slova anglicky

protein Mgm101; linear mitochondrial genome

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2021 10:15, Mgr. Tereza Miškechová

Anotace

V originále

To study the mechanisms involved in the maintenance of a linear mitochondrial genome we investigated the biochemical properties of the recombination protein Mgm101 from Candida parapsilosis. We show that CpMgm101 complements defects associated with the Saccharomyces cerevisiaemgm101–1ts mutation and that it is present in both the nucleus and mitochondrial nucleoids of C. parapsilosis. Unlike its S. cerevisiae counterpart, CpMgm101 is associated with the entire nucleoid population and is able to bind to a broad range of DNA substrates in a non-sequence specific manner. CpMgm101 is also able to catalyze strand annealing and D-loop formation. CpMgm101 forms a roughly C-shaped trimer in solution according to SAXS. Electron microscopy of a complex of CpMgm101 with a model mitochondrial telomere revealed homogeneous, ring-shaped structures at the telomeric single-stranded overhangs. The DNA-binding properties of CpMgm101, together with its DNA recombination properties, suggest that it can play a number of possible roles in the replication of the mitochondrial genome and the maintenance of its telomeres.

Návaznosti

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

Citovat

PEVALA, Vladimír, Dominika TRUBAN, Jacob A. BAUER, Július KOŠŤAN, Nina KUNOVÁ, Jana BELLOVÁ, Marlene BRANDSTETTER, María Victoria MARINI PALOMEQUE, Lumír KREJČÍ, Ľubomír TOMÁŠKA, Jozef NOSEK a Eva KUTEJOVÁ. The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome. Nucleic Acids Research. Oxford: Oxford University Press, 2016, roč. 44, č. 5, s. 2227-2239. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv1529.

@article{1337124,
   author = {Pevala, Vladimír and Truban, Dominika and Bauer, Jacob A. and Košťan, Július and Kunová, Nina and Bellová, Jana and Brandstetter, Marlene and Marini Palomeque, María Victoria and Krejčí, Lumír and Tomáška, Ľubomír and Nosek, Jozef and Kutejová, Eva},
   article_location = {Oxford},
   article_number = {5},
   doi = {http://dx.doi.org/10.1093/nar/gkv1529},
   keywords = {protein Mgm101; linear mitochondrial genome},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome},
   url = {https://watermark.silverchair.com/gkv1529.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAq0wggKpBgkqhkiG9w0BBwagggKaMIIClgIBADCCAo8GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM4kUtPj3Aq2Od7asfAgEQgIICYGBChiYEKaG14NNQO0W0Q9TEu--q6Ht1TqTUtnPPPzb_5p},
   volume = {44},
   year = {2016}
}

TY  - JOUR
ID  - 1337124
AU  - Pevala, Vladimír - Truban, Dominika - Bauer, Jacob A. - Košťan, Július - Kunová, Nina - Bellová, Jana - Brandstetter, Marlene - Marini Palomeque, María Victoria - Krejčí, Lumír - Tomáška, Ľubomír - Nosek, Jozef - Kutejová, Eva
PY  - 2016
TI  - The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome
JF  - Nucleic Acids Research
VL  - 44
IS  - 5
SP  - 2227-2239
EP  - 2227-2239
PB  - Oxford University Press
SN  - 03051048
KW  - protein Mgm101
KW  - linear mitochondrial genome
UR  - https://watermark.silverchair.com/gkv1529.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ysgAAAq0wggKpBgkqhkiG9w0BBwagggKaMIIClgIBADCCAo8GCSqGSIb3DQEHATAeBglghkgBZQMEAS4wEQQM4kUtPj3Aq2Od7asfAgEQgIICYGBChiYEKaG14NNQO0W0Q9TEu--q6Ht1TqTUtnPPPzb_5p
N2  - To study the mechanisms involved in the maintenance of a linear mitochondrial genome we investigated the biochemical properties of the recombination protein Mgm101 from Candida parapsilosis. We show that CpMgm101 complements defects associated with the Saccharomyces cerevisiaemgm101–1ts mutation and that it is present in both the nucleus and mitochondrial nucleoids of C. parapsilosis. Unlike its S. cerevisiae counterpart, CpMgm101 is associated with the entire nucleoid population and is able to bind to a broad range of DNA substrates in a non-sequence specific manner. CpMgm101 is also able to catalyze strand annealing and D-loop formation. CpMgm101 forms a roughly C-shaped trimer in solution according to SAXS. Electron microscopy of a complex of CpMgm101 with a model mitochondrial telomere revealed homogeneous, ring-shaped structures at the telomeric single-stranded overhangs. The DNA-binding properties of CpMgm101, together with its DNA recombination properties, suggest that it can play a number of possible roles in the replication of the mitochondrial genome and the maintenance of its telomeres.
ER  -

PEVALA, Vladimír, Dominika TRUBAN, Jacob A. BAUER, Július KOŠŤAN, Nina KUNOVÁ, Jana BELLOVÁ, Marlene BRANDSTETTER, María Victoria MARINI PALOMEQUE, Lumír KREJČÍ, Ľubomír TOMÁŠKA, Jozef NOSEK a Eva KUTEJOVÁ. The structure and DNA-binding properties of Mgm101 from a yeast with a linear mitochondrial genome. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2016, roč.~44, č.~5, s.~2227-2239. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv1529.

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