Cryptochrome 2 mediates directional magnetoreception in
cockroaches

J 2016

Cryptochrome 2 mediates directional magnetoreception in cockroaches

BAZALOVÁ, Olga, Markéta KVÍČALOVÁ, Tereza VÁLKOVÁ, Pavel SLABÝ, Přemysl BARTOŠ et. al.

Basic information

Original name

Cryptochrome 2 mediates directional magnetoreception in cockroaches

Authors

BAZALOVÁ, Olga (203 Czech Republic), Markéta KVÍČALOVÁ (203 Czech Republic, belonging to the institution), Tereza VÁLKOVÁ (203 Czech Republic, belonging to the institution), Pavel SLABÝ (203 Czech Republic, belonging to the institution), Přemysl BARTOŠ (203 Czech Republic, belonging to the institution), Radek NETUŠIL (203 Czech Republic, belonging to the institution), Kateřina TOMANOVÁ (203 Czech Republic, belonging to the institution), Peter BRAEUNIG (276 Germany), How-Jing LEE (158 Taiwan), Ivo ŠAUMAN (203 Czech Republic), Milena DAMULEWICZ (616 Poland), Jan PROVAZNIK (203 Czech Republic), Richard POKORNY (703 Slovakia), David DOLEZEL (203 Czech Republic) and Martin VÁCHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2016, 0027-8424

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 9.661

RIV identification code

RIV/00216224:14310/16:00087833

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1073/pnas.1518622113

UT WoS

000369571700058

Keywords in English

magnetoreception; cryptochrome; light spectrum; locomotor activity; circadian genes

Abstract

V originále

The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD(.-) and FADH(-) redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system.

Links

GC13-11908J, research and development project

Name: Fyziologická a funkčně genetická analýza magnetorecepce na hmyzím modelu. (Acronym: Magnet)

Investor: Czech Science Foundation

GC206/07/J041, research and development project

Name: Neurální podstata magnetorecepce hmyzu.

Investor: Czech Science Foundation, Towards the neuronal basis of insect magnetoreception

Citovat

BAZALOVÁ, Olga, Markéta KVÍČALOVÁ, Tereza VÁLKOVÁ, Pavel SLABÝ, Přemysl BARTOŠ, Radek NETUŠIL, Kateřina TOMANOVÁ, Peter BRAEUNIG, How-Jing LEE, Ivo ŠAUMAN, Milena DAMULEWICZ, Jan PROVAZNIK, Richard POKORNY, David DOLEZEL and Martin VÁCHA. Cryptochrome 2 mediates directional magnetoreception in cockroaches. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2016, vol. 113, No 6, p. 1660-1665. ISSN 0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1518622113.

@article{1338176,
   author = {Bazalová, Olga and Kvíčalová, Markéta and Válková, Tereza and Slabý, Pavel and Bartoš, Přemysl and Netušil, Radek and Tomanová, Kateřina and Braeunig, Peter and Lee, HowandJing and Šauman, Ivo and Damulewicz, Milena and Provaznik, Jan and Pokorny, Richard and Dolezel, David and Vácha, Martin},
   article_location = {WASHINGTON},
   article_number = {6},
   doi = {http://dx.doi.org/10.1073/pnas.1518622113},
   keywords = {magnetoreception; cryptochrome; light spectrum; locomotor activity; circadian genes},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Cryptochrome 2 mediates directional magnetoreception in cockroaches},
   volume = {113},
   year = {2016}
}

TY  - JOUR
ID  - 1338176
AU  - Bazalová, Olga - Kvíčalová, Markéta - Válková, Tereza - Slabý, Pavel - Bartoš, Přemysl - Netušil, Radek - Tomanová, Kateřina - Braeunig, Peter - Lee, How-Jing - Šauman, Ivo - Damulewicz, Milena - Provaznik, Jan - Pokorny, Richard - Dolezel, David - Vácha, Martin
PY  - 2016
TI  - Cryptochrome 2 mediates directional magnetoreception in cockroaches
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 113
IS  - 6
SP  - 1660-1665
EP  - 1660-1665
PB  - National Academy of Sciences
SN  - 00278424
KW  - magnetoreception
KW  - cryptochrome
KW  - light spectrum
KW  - locomotor activity
KW  - circadian genes
N2  - The ability to perceive geomagnetic fields (GMFs) represents a fascinating biological phenomenon. Studies on transgenic flies have provided evidence that photosensitive Cryptochromes (Cry) are involved in the response to magnetic fields (MFs). However, none of the studies tackled the problem of whether the Cry-dependent magnetosensitivity is coupled to the sole MF presence or to the direction of MF vector. In this study, we used gene silencing and a directional MF to show that mammalian-like Cry2 is necessary for a genuine directional response to periodic rotations of the GMF vector in two insect species. Longer wavelengths of light required higher photon fluxes for a detectable behavioral response, and a sharp detection border was present in the cyan/green spectral region. Both observations are consistent with involvement of the FADox, FAD(.-) and FADH(-) redox forms of flavin. The response was lost upon covering the eyes, demonstrating that the signal is perceived in the eye region. Immunohistochemical staining detected Cry2 in the hemispherical layer of laminal glia cells underneath the retina. Together, these findings identified the eye-localized Cry2 as an indispensable component and a likely photoreceptor of the directional GMF response. Our study is thus a clear step forward in deciphering the in vivo effects of GMF and supports the interaction of underlying mechanism with the visual system.
ER  -

BAZALOVÁ, Olga, Markéta KVÍČALOVÁ, Tereza VÁLKOVÁ, Pavel SLABÝ, Přemysl BARTOŠ, Radek NETUŠIL, Kateřina TOMANOVÁ, Peter BRAEUNIG, How-Jing LEE, Ivo ŠAUMAN, Milena DAMULEWICZ, Jan PROVAZNIK, Richard POKORNY, David DOLEZEL and Martin VÁCHA. Cryptochrome 2 mediates directional magnetoreception in cockroaches. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2016, vol.~113, No~6, p.~1660-1665. ISSN~0027-8424. Available from: https://dx.doi.org/10.1073/pnas.1518622113.

Detailed Information on Publication Record