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@article{1340474,
author = {Celá, Andrea and Mádr, Aleš and Dědová, Tereza and Pelcová, Marta and Ješeta, Michal and Žáková, Jana and Crha, Igor and Glatz, Zdeněk},
article_number = {17-18},
doi = {http://dx.doi.org/10.1002/elps.201500587},
keywords = {assisted reproduction; MEKC; LIF; amino acids},
language = {eng},
issn = {0173-0835},
journal = {Electrophoresis},
title = {MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization},
volume = {37},
year = {2016}
}
TY - JOUR
ID - 1340474
AU - Celá, Andrea - Mádr, Aleš - Dědová, Tereza - Pelcová, Marta - Ješeta, Michal - Žáková, Jana - Crha, Igor - Glatz, Zdeněk
PY - 2016
TI - MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization
JF - Electrophoresis
VL - 37
IS - 17-18
SP - 2305-2312
EP - 2305-2312
PB - Wiley
SN - 01730835
KW - assisted reproduction
KW - MEKC
KW - LIF
KW - amino acids
N2 - Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo’s physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo’s surroundings can determine the embryo’s physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo’s surroundings was developed. AAs were analyzed by means of MEKC-LIF after on capillary derivatization by naphthalene 2,3 dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology. The resulting derivatives of all the standard AAs were baseline resolved in the background electrolyte comprised of 35 mM sodium tetraborate, 55 mM SDS, 2.7 M urea, 1 mM BIS-TRIS propane, and 23 mM NaOH within 50 minutes. The method was applied on an analysis of spent commercially available culture media used in assisted reproduction to culture embryos after in vitro fertilization.
ER -
CELÁ, Andrea, Aleš MÁDR, Tereza DĚDOVÁ, Marta PELCOVÁ, Michal JEŠETA, Jana ŽÁKOVÁ, Igor CRHA and Zdeněk GLATZ. MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization. \textit{Electrophoresis}. Wiley, 2016, vol.~37, 17-18, p.~2305-2312. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.201500587.
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