Marine amoebae with cytoplasmic and perinuclear symbionts
deeply branching in the Gammaproteobacteria

J 2015

Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria

SCHULZ, Frederik, Tomáš TYML, Ilaria PIZZETTI, Iva DYKOVÁ, Stefano FAZI et. al.

Základní údaje

Originální název

Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria

Autoři

SCHULZ, Frederik (40 Rakousko), Tomáš TYML (203 Česká republika, domácí), Ilaria PIZZETTI (380 Itálie), Iva DYKOVÁ (203 Česká republika, garant, domácí), Stefano FAZI (380 Itálie), Martin KOSTKA (203 Česká republika) a Matthias HORN (40 Rakousko)

Vydání

Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2015, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.228

Kód RIV

RIV/00216224:14310/15:00081664

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/srep13381

UT WoS

000360035600002

Klíčová slova anglicky

Microbial ecology; symbiosis

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 3. 2018 14:12, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named 'Candidatus Occultobacter vannellae' and 'Candidatus Nucleophilum amoebae'. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85-88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

SCHULZ, Frederik, Tomáš TYML, Ilaria PIZZETTI, Iva DYKOVÁ, Stefano FAZI, Martin KOSTKA a Matthias HORN. Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2015, roč. 5, August, s. "nestránkováno", 10 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep13381.

@article{1340509,
   author = {Schulz, Frederik and Tyml, Tomáš and Pizzetti, Ilaria and Dyková, Iva and Fazi, Stefano and Kostka, Martin and Horn, Matthias},
   article_location = {LONDON},
   article_number = {August},
   doi = {http://dx.doi.org/10.1038/srep13381},
   keywords = {Microbial ecology; symbiosis},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria},
   url = {http://www.nature.com/articles/srep13381},
   volume = {5},
   year = {2015}
}

TY  - JOUR
ID  - 1340509
AU  - Schulz, Frederik - Tyml, Tomáš - Pizzetti, Ilaria - Dyková, Iva - Fazi, Stefano - Kostka, Martin - Horn, Matthias
PY  - 2015
TI  - Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria
JF  - Scientific Reports
VL  - 5
IS  - August
SP  - "nestránkováno"
EP  - "nestránkováno"
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - Microbial ecology
KW  - symbiosis
UR  - http://www.nature.com/articles/srep13381
L2  - http://www.nature.com/articles/srep13381
N2  - Amoebae play an important ecological role as predators in microbial communities. They also serve as niche for bacterial replication, harbor endosymbiotic bacteria and have contributed to the evolution of major human pathogens. Despite their high diversity, marine amoebae and their association with bacteria are poorly understood. Here we describe the isolation and characterization of two novel marine amoebae together with their bacterial endosymbionts, tentatively named 'Candidatus Occultobacter vannellae' and 'Candidatus Nucleophilum amoebae'. While one amoeba strain is related to Vannella, a genus common in marine habitats, the other represents a novel lineage in the Amoebozoa. The endosymbionts showed only low similarity to known bacteria (85-88% 16S rRNA sequence similarity) but together with other uncultured marine bacteria form a sister clade to the Coxiellaceae. Using fluorescence in situ hybridization and transmission electron microscopy, identity and intracellular location of both symbionts were confirmed; one was replicating in host-derived vacuoles, whereas the other was located in the perinuclear space of its amoeba host. This study sheds for the first time light on a so far neglected group of protists and their bacterial symbionts. The newly isolated strains represent easily maintainable model systems and pave the way for further studies on marine associations between amoebae and bacterial symbionts.
ER  -

SCHULZ, Frederik, Tomáš TYML, Ilaria PIZZETTI, Iva DYKOVÁ, Stefano FAZI, Martin KOSTKA a Matthias HORN. Marine amoebae with cytoplasmic and perinuclear symbionts deeply branching in the Gammaproteobacteria. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2015, roč.~5, August, s.~''nestránkováno'', 10 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep13381.

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