ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN a Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. Nucleic Acids Research. Oxford: Oxford University Press, 2016, roč. 44, č. 3, s. 1064-1079. ISSN 0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv1021. |
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@article{1340844, author = {Zábrady, Kateřina and Adamus, Marek and Vondrová, Lucie and Liao, Chunyan and Skoupilová, Hana and Nováková, Markéta and Jurčišinová, Lenka and Alt, Aaron and Oliver, Antony W. and Lehmann, Alan R. and Paleček, Jan}, article_location = {Oxford}, article_number = {3}, doi = {http://dx.doi.org/10.1093/nar/gkv1021}, keywords = {SMC5-SMC6 COMPLEX; FISSION YEAST; SACCHAROMYCES-CEREVISIAE; CORE COMPONENT; REPAIR COMPLEX; ATP HYDROLYSIS; PROTEINS; COHESIN; REPLICATION; CHROMOSOMES}, language = {eng}, issn = {0305-1048}, journal = {Nucleic Acids Research}, title = {Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA}, url = {https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021}, volume = {44}, year = {2016} }
TY - JOUR ID - 1340844 AU - Zábrady, Kateřina - Adamus, Marek - Vondrová, Lucie - Liao, Chunyan - Skoupilová, Hana - Nováková, Markéta - Jurčišinová, Lenka - Alt, Aaron - Oliver, Antony W. - Lehmann, Alan R. - Paleček, Jan PY - 2016 TI - Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA JF - Nucleic Acids Research VL - 44 IS - 3 SP - 1064-1079 EP - 1064-1079 PB - Oxford University Press SN - 03051048 KW - SMC5-SMC6 COMPLEX KW - FISSION YEAST KW - SACCHAROMYCES-CEREVISIAE KW - CORE COMPONENT KW - REPAIR COMPLEX KW - ATP HYDROLYSIS KW - PROTEINS KW - COHESIN KW - REPLICATION KW - CHROMOSOMES UR - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021 L2 - https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkv1021 N2 - SMC5/6 is a highly conserved protein complex related to cohesin and condensin, which are the key components of higher-order chromatin structures. The SMC5/6 complex is essential for proliferation in yeast and is involved in replication fork stability and processing. However, the precise mechanism of action of SMC5/6 is not known. Here we present evidence that the NSE1/NSE3/NSE4 sub-complex of SMC5/6 binds to double-stranded DNA without any preference for DNA-replication/recombination intermediates. Mutations of key basic residues within the NSE1/NSE3/NSE4 DNA-binding surface reduce binding to DNA in vitro. Their introduction into the Schizosaccharomyces pombe genome results in cell death or hypersensitivity to DNA damaging agents. Chromatin immunoprecipitation analysis of the hypomorphic nse3 DNA-binding mutant shows a reduced association of fission yeast SMC5/6 with chromatin. Based on our results, we propose a model for loading of the SMC5/6 complex onto the chromatin. ER -
ZÁBRADY, Kateřina, Marek ADAMUS, Lucie VONDROVÁ, Chunyan LIAO, Hana SKOUPILOVÁ, Markéta NOVÁKOVÁ, Lenka JURČIŠINOVÁ, Aaron ALT, Antony W. OLIVER, Alan R. LEHMANN a Jan PALEČEK. Chromatin association of the SMC5/6 complex is dependent on binding of its NSE3 subunit to DNA. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2016, roč.~44, č.~3, s.~1064-1079. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkv1021.
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