Localized Movement and Levels of 53BP1 Protein Are Changed by
gamma-irradiation in PML Deficient Cells

J 2016

Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells

LEGARTOVÁ, Soňa, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Barbora MALYŠKOVÁ, Thomas KÜNTZIGER, Philippe COLLAS et. al.

Základní údaje

Originální název

Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells

Autoři

LEGARTOVÁ, Soňa (703 Slovensko), Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ (203 Česká republika), Barbora MALYŠKOVÁ (203 Česká republika), Thomas KÜNTZIGER (578 Norsko), Philippe COLLAS (578 Norsko), Dušan CMARKO (203 Česká republika), Ivan RAŠKA (203 Česká republika), Dmitry SOROKIN (643 Rusko, garant, domácí), Stanislav KOZUBEK (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Journal of Cellular Biochemistry [IF=2.778 (1990)], Wiley-Liss, 2016, 0730-2312

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.085

Kód RIV

RIV/00216224:14330/16:00087865

Organizační jednotka

Fakulta informatiky

DOI

http://dx.doi.org/10.1002/jcb.25551

UT WoS

000383626800015

Klíčová slova anglicky

DNA REPAIR; 53BP1 PROTEIN; PML BODIES; HISTONE MODIFICATIONS; MICROSCOPY; PHOTOBLEACHING

Štítky

CBIA, cbia-web

Změněno: 27. 4. 2017 05:49, RNDr. Pavel Šmerk, Ph.D.

Anotace

V originále

We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and g-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after g-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Citovat

LEGARTOVÁ, Soňa, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Barbora MALYŠKOVÁ, Thomas KÜNTZIGER, Philippe COLLAS, Dušan CMARKO, Ivan RAŠKA, Dmitry SOROKIN, Stanislav KOZUBEK a Eva BÁRTOVÁ. Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells. Journal of Cellular Biochemistry [IF=2.778 (1990)]. Wiley-Liss, 2016, roč. 117, č. 11, s. 2583-2596. ISSN 0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.25551.

@article{1340931,
   author = {Legartová, Soňa and ŘezníčkováandPodloučková, Petra and Malyšková, Barbora and Küntziger, Thomas and Collas, Philippe and Cmarko, Dušan and Raška, Ivan and Sorokin, Dmitry and Kozubek, Stanislav and Bártová, Eva},
   article_number = {11},
   doi = {http://dx.doi.org/10.1002/jcb.25551},
   keywords = {DNA REPAIR; 53BP1 PROTEIN; PML BODIES; HISTONE MODIFICATIONS; MICROSCOPY; PHOTOBLEACHING},
   language = {eng},
   issn = {0730-2312},
   journal = {Journal of Cellular Biochemistry [IF=2.778 (1990)]},
   title = {Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells},
   volume = {117},
   year = {2016}
}

TY  - JOUR
ID  - 1340931
AU  - Legartová, Soňa - Řezníčková-Podloučková, Petra - Malyšková, Barbora - Küntziger, Thomas - Collas, Philippe - Cmarko, Dušan - Raška, Ivan - Sorokin, Dmitry - Kozubek, Stanislav - Bártová, Eva
PY  - 2016
TI  - Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells
JF  - Journal of Cellular Biochemistry [IF=2.778 (1990)]
VL  - 117
IS  - 11
SP  - 2583-2596
EP  - 2583-2596
PB  - Wiley-Liss
SN  - 07302312
KW  - DNA REPAIR
KW  - 53BP1 PROTEIN
KW  - PML BODIES
KW  - HISTONE MODIFICATIONS
KW  - MICROSCOPY
KW  - PHOTOBLEACHING
N2  - We studied epigenetics, distribution pattern, kinetics, and diffusion of proteins recruited to spontaneous and g-radiation-induced DNA lesions. We showed that PML deficiency leads to an increased number of DNA lesions, which was accompanied by changes in histone signature. In PML wt cells, we observed two mobile fractions of 53BP1 protein with distinct diffusion in spontaneous lesions. These protein fractions were not detected in PML-deficient cells, characterized by slow-diffusion of 53BP1. Single particle tracking analysis revealed limited local motion of 53BP1 foci in PML double null cells and local motion 53BP1 foci was even more reduced after g-irradiation. However, radiation did not change co-localization between 53BP1 nuclear bodies and interchromatin granule-associated zones (IGAZs), nuclear speckles, or chromocenters. This newly observed interaction pattern imply that 53BP1 protein could be a part of not only DNA repair, but also process mediated via components accumulated in IGAZs, nuclear speckles, or paraspeckles. Together, PML deficiency affected local motion of 53BP1 nuclear bodies and changed composition and a number of irradiation-induced foci.
ER  -

LEGARTOVÁ, Soňa, Petra ŘEZNÍČKOVÁ-PODLOUČKOVÁ, Barbora MALYŠKOVÁ, Thomas KÜNTZIGER, Philippe COLLAS, Dušan CMARKO, Ivan RAŠKA, Dmitry SOROKIN, Stanislav KOZUBEK a Eva BÁRTOVÁ. Localized Movement and Levels of 53BP1 Protein Are Changed by gamma-irradiation in PML Deficient Cells. \textit{Journal of Cellular Biochemistry [IF=2.778 (1990)]}. Wiley-Liss, 2016, roč.~117, č.~11, s.~2583-2596. ISSN~0730-2312. Dostupné z: https://dx.doi.org/10.1002/jcb.25551.

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