J 2015

Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea

TRAN, Trunk D., Hieu X. CAO, Gabriele JOVTCHEV, Pavel NEUMANN, Petr NOVÁK et. al.

Basic information

Original name

Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea

Authors

TRAN, Trunk D. (276 Germany), Hieu X. CAO (276 Germany), Gabriele JOVTCHEV (276 Germany), Pavel NEUMANN (203 Czech Republic), Petr NOVÁK (203 Czech Republic), Miloslava FOJTOVÁ (203 Czech Republic, guarantor, belonging to the institution), Giang T. H. VU (276 Germany), Jiří MACAS (203 Czech Republic), Jiří FAJKUS (203 Czech Republic, belonging to the institution), Ingo SCHUBERT (276 Germany, belonging to the institution) and Joerg FUCHS (276 Germany)

Edition

Plant Journal, Hoboken (USA), Wiley-Blackwell, 2015, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.468

RIV identification code

RIV/00216224:14740/15:00081701

Organization unit

Central European Institute of Technology

DOI

UT WoS

000368268700004

Keywords in English

Lentibulariaceae; Genlisea nigrocaulis; G. hispidula; centromeric tandem repeat; centromeric retrotransposons; plant telomeric repeat variants; telomerase; genome evolution

Tags

Změněno: 12/4/2016 16:34, Mgr. Eva Špillingová

Abstract

V originále

Linear chromosomes of eukaryotic organisms invariably possess centromeres and telomeres to ensure proper chromosome segregation during nuclear divisions and to protect the chromosome ends from deterioration and fusion, respectively. While centromeric sequences may differ between species, with arrays of tandemly repeated sequences and retrotransposons being the most abundant sequence types in plant centromeres, telomeric sequences are usually highly conserved among plants and other organisms. The genome size of the carnivorous genus Genlisea (Lentibulariaceae) is highly variable. Here we study evolutionary sequence plasticity of these chromosomal domains at an intrageneric level. We show that Genlisea nigrocaulis (1C = 86 Mbp; 2n = 40) and G. hispidula (1C = 1550 Mbp; 2n = 40) differ as to their DNA composition at centromeres and telomeres. G. nigrocaulis and its close relative G. pygmaea revealed mainly 161 bp tandem repeats, while G. hispidula and its close relative G. subglabra displayed a combination of four retroelements at centromeric positions. G. nigrocaulis and G. pygmaea chromosome ends are characterized by the Arabidopsis-type telomeric repeats (TTTAGGG); G. hispidula and G. subglabra instead revealed two intermingled sequence variants (TTCAGG and TTTCAGG). These differences in centromeric and, surprisingly, also in telomeric DNA sequences, uncovered between groups with on average a > 9-fold genome size difference, emphasize the fast genome evolution within this genus. Such intrageneric evolutionary alteration of telomeric repeats with cytosine in the guanine-rich strand, not yet known for plants, might impact the epigenetic telomere chromatin modification.

Links

EE2.3.20.0189, research and development project
Name: Rozvoj výzkumné excelence v oblasti evoluční cytogenomiky, epigenetiky a buněčné signalizace
GA13-06943S, research and development project
Name: Strukturní a funkční komponenty rostlinných telomer
Investor: Czech Science Foundation
GBP501/12/G090, research and development project
Name: Evoluce a funkce komplexních genomů rostlin