Composition of cultivable enteric bacteria from the intestine
of Antarctic fish (family Nototheniidae)

J 2016

Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)

SEDLÁČEK, Ivo, Eva STAŇKOVÁ a Pavel ŠVEC

Základní údaje

Originální název

Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)

Autoři

SEDLÁČEK, Ivo (203 Česká republika, garant, domácí), Eva STAŇKOVÁ (203 Česká republika, domácí) a Pavel ŠVEC (203 Česká republika, domácí)

Vydání

Czech Journal of Animal Science, 2016, 1212-1819

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.741

Kód RIV

RIV/00216224:14310/16:00089679

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.17221/8785-CJAS

UT WoS

000375410300004

Klíčová slova anglicky

gut microflora; selective media; identification; Enterobacter; Aeromonas

Štítky

AKR, rivok

Změněno: 13. 3. 2018 23:30, prof. RNDr. Ivo Sedláček, CSc.

Anotace

V originále

In this study, the intestinal bacteria of wild Antarctic fish (family Nototeniidae) were examined with traditional culture-based techniques. The bacterial flora of the gut contents of four marine fish species Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni and Trematomus newnesi has been investigated in an attempt to describe the allochthonous bacteria inhabiting these Nototheniidae fish. A set of 43 fresh and healthy fish was analysed and intestinal bacteria were retrieved using the dilution plate technique on selective media Endo agar and XLDA agar. A total of 133 different bacterial isolates were obtained and initially characterised by key phenotypical tests. Notothenoid fish gut microbiota showed low species diversity of isolates and intestines were inhabited in average amount of 3 different isolates per fish. The poor bacterial colonization of intestine content showed samples of Trematomus newnesi. Curiously, the Gram-negative non-fermenters, including Pseudomonas sp., Vibrio sp. and Alcaligenes-like isolates represented the majority (59%) of intestine isolates grown on the used selective media for enteric bacteria. Based on preliminary identification, only 54 isolates (41%) were tentatively determined as enteric bacteria. The identification of 54 isolates of Gram-negative fermenting rods to the species level was achieved using biochemical characterization by commercial kits ENTEROtest 24 and Biolog GN2 MicroPlate. Results showed that Enterobacter cloacae phenon and Aeromonas hydrophila were predominant bacterial species in the free-living fish intestine from the group of fermenting Gram-negative rods.

Návaznosti

LM2010009, projekt VaV

Název: Projekt CzechPolar - České polární stanice: Stavba a operační náklady

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Czech Polar - České polární stanice: Stavba a operační náklady

Citovat

SEDLÁČEK, Ivo, Eva STAŇKOVÁ a Pavel ŠVEC. Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae). Czech Journal of Animal Science. 2016, roč. 61, č. 3, s. 127-132. ISSN 1212-1819. Dostupné z: https://dx.doi.org/10.17221/8785-CJAS.

@article{1342209,
   author = {Sedláček, Ivo and Staňková, Eva and Švec, Pavel},
   article_number = {3},
   doi = {http://dx.doi.org/10.17221/8785-CJAS},
   keywords = {gut microflora; selective media; identification; Enterobacter; Aeromonas},
   language = {eng},
   issn = {1212-1819},
   journal = {Czech Journal of Animal Science},
   title = {Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)},
   volume = {61},
   year = {2016}
}

TY  - JOUR
ID  - 1342209
AU  - Sedláček, Ivo - Staňková, Eva - Švec, Pavel
PY  - 2016
TI  - Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae)
JF  - Czech Journal of Animal Science
VL  - 61
IS  - 3
SP  - 127-132
EP  - 127-132
SN  - 12121819
KW  - gut microflora
KW  - selective media
KW  - identification
KW  - Enterobacter
KW  - Aeromonas
N2  - In this study, the intestinal bacteria of wild Antarctic fish (family Nototeniidae) were examined with traditional culture-based techniques. The bacterial flora of the gut contents of four marine fish species Notothenia coriiceps, Trematomus bernacchii, Trematomus hansoni and Trematomus newnesi has been investigated in an attempt to describe the allochthonous bacteria inhabiting these Nototheniidae fish. A set of 43 fresh and healthy fish was analysed and intestinal bacteria were retrieved using the dilution plate technique on selective media Endo agar and XLDA agar. A total of 133 different bacterial isolates were obtained and initially characterised by key phenotypical tests. Notothenoid fish gut microbiota showed low species diversity of isolates and intestines were inhabited in average amount of 3 different isolates per fish. The poor bacterial colonization of intestine content showed samples of Trematomus newnesi. Curiously, the Gram-negative non-fermenters, including Pseudomonas sp., Vibrio sp. and Alcaligenes-like isolates represented the majority (59%) of intestine isolates grown on the used selective media for enteric bacteria. Based on preliminary identification, only 54 isolates (41%) were tentatively determined as enteric bacteria. The identification of 54 isolates of Gram-negative fermenting rods to the species level was achieved using biochemical characterization by commercial kits ENTEROtest 24 and Biolog GN2 MicroPlate. Results showed that Enterobacter cloacae phenon and Aeromonas hydrophila were predominant bacterial species in the free-living fish intestine from the group of fermenting Gram-negative rods.
ER  -

SEDLÁČEK, Ivo, Eva STAŇKOVÁ a Pavel ŠVEC. Composition of cultivable enteric bacteria from the intestine of Antarctic fish (family Nototheniidae). \textit{Czech Journal of Animal Science}. 2016, roč.~61, č.~3, s.~127-132. ISSN~1212-1819. Dostupné z: https://dx.doi.org/10.17221/8785-CJAS.

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