Další formáty:
BibTeX
LaTeX
RIS
@article{1342213,
author = {Zanni, Giulia and Di Martino, Elena and Omelyanenko, Anna and Andäng, Michael and Delle, Ulla and Elmroth, Kecke and Blomgren, Klas},
article_location = {Albany},
article_number = {35},
keywords = {lithium; hippocampus; radiotherapy; apoptosis; paediatric oncology},
language = {eng},
issn = {1949-2553},
journal = {Oncotarget},
title = {Lithium increases proliferation of hippocampal neural stem/progenitor cells and rescues irradiation-induced cell cycle arrest in vitro},
url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741917/},
volume = {6},
year = {2015}
}
TY - JOUR
ID - 1342213
AU - Zanni, Giulia - Di Martino, Elena - Omelyanenko, Anna - Andäng, Michael - Delle, Ulla - Elmroth, Kecke - Blomgren, Klas
PY - 2015
TI - Lithium increases proliferation of hippocampal neural stem/progenitor cells and rescues irradiation-induced cell cycle arrest in vitro
JF - Oncotarget
VL - 6
IS - 35
SP - 37083-37097
EP - 37083-37097
PB - Impact Journals
SN - 19492553
KW - lithium
KW - hippocampus
KW - radiotherapy
KW - apoptosis
KW - paediatric oncology
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741917/
L2 - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4741917/
N2 - Radiotherapy in children causes debilitating cognitive decline, partly linked to impaired neurogenesis. Irradiation targets primarily cancer cells but also endogenous neural stem/progenitor cells (NSPCs) leading to cell death or cell cycle arrest. Here we evaluated the effects of lithium on proliferation, cell cycle and DNA damage after irradiation of young NSPCs in vitro. NSPCs were treated with 1 or 3 mM LiCl and we investigated proliferation capacity (neurosphere volume and bromodeoxyuridine (BrdU) incorporation). Using flow cytometry, we analysed apoptosis (annexin V), cell cycle (propidium iodide) and DNA damage (gamma H2AX) after irradiation (3.5 Gy) of lithium-treated NSPCs. Lithium increased BrdU incorporation and, dose-dependently, the number of cells in replicative phase as well as neurosphere growth. Irradiation induced cell cycle arrest in G(1) and G(2)/M phases. Treatment with 3 mM LiCl was sufficient to increase NSPCs in S phase, boost neurosphere growth and reduce DNA damage. Lithium did not affect the levels of apoptosis, suggesting that it does not rescue NSPCs committed to apoptosis due to accumulated DNA damage. Lithium is a very promising candidate for protection of the juvenile brain from radiotherapy and for its potential to thereby improve the quality of life for those children who survive their cancer.
ER -
ZANNI, Giulia, Elena DI MARTINO, Anna OMELYANENKO, Michael ANDÄNG, Ulla DELLE, Kecke ELMROTH a Klas BLOMGREN. Lithium increases proliferation of hippocampal neural stem/progenitor cells and rescues irradiation-induced cell cycle arrest in vitro. \textit{Oncotarget}. Albany: Impact Journals, 2015, roč.~6, č.~35, s.~37083-37097. ISSN~1949-2553.
|
