DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL
CELL CARCINOMA BY NGS

a 2016

DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS

ILIEV, Robert, Petra VYCHYTILOVÁ, Zuzana OŽANOVÁ, Silvia RYBECKÁ, Lenka RADOVÁ et. al.

Základní údaje

Originální název

DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS

Autoři

ILIEV, Robert, Petra VYCHYTILOVÁ, Zuzana OŽANOVÁ, Silvia RYBECKÁ, Lenka RADOVÁ, Michal STANÍK, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK a Ondřej SLABÝ

Vydání

XL. brněnské onkologické dny a XXX. koncerence pro nelékařské zdravotnické pracovníky, Brno, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Středoevropský technologický institut

ISSN

Klíčová slova česky

miRNA; piRNA; renální karcinom; sekvenování nové generace

Změněno: 4. 5. 2016 10:57, Mgr. Petra Vychytilová, Ph.D.

Anotace

V originále

Introduction: MicroRNAs (miRNAs) are the class of small non-cod ing RNAs long 21– 25 nucleotides. They play an important role in regulation of transcription. They affect gene expres sion at post-transcriptional levels through binding to complementary mRNAs and mediate their degradation in RISC complex. Piwi-interacting RNAs (piRNAs) is newly discovered class of small non-cod ing RNA. They are single-stranded RNAs long 26– 31 nucleotides. They are involved in silencing of transposable elements and also participate in sequence-specific chromatin modifications. Deregulation of piRNAs was observed in kidney, bladder, gastric, breast, pancreatic and liver cancers. Patients and Methods: In our study we used the tumor tis sue and the paired adjacent non-tumor renal parenchyma of 12 patients (7 males and 5 females) with renal cell carcinoma (RCC) hospitalized in Masaryk Memorial Cancer Institute. RNA was isolated with mirVana™ miRNA Isolation Kit. For prepar ing RNA library was used TruSeq Small RNA Sample Preparation Kit from Il lumina and then the miSeq sequencing technology was used to detect small RNAs. Results: In our 12 paired samples of tumor tis sue and the paired adjacent non-tumor renal parenchyma we detected 283 miRNAs with > 1 read in at least 13 samples. 55 miRNAs were statistically significant different expressed (p < 0.05) in tumor tissue then in adjacent non-tumor parenchyma. MiRNAs with most significant altered expression (p < 0.01) were miR-129, miR-138, miR-142, miR-149, miR-154, miR-155, miR-200b, miR-210, miR-218, miR-340, miR-584, miR-885, miR-891a, miR-1270, miR-3690 and miR-7641. After piRNA sequences analysis we found 440 piRNAs with > 1 read in at least 13 samples. From these piRNAs were 38 piRNAs statistical ly signifi cant deregulated (p < 0.01). Most statisticaly significant altered expression levels were observed in piR-1207, piR-2107, piR-2155, piR-12487, piR-12488, piR-21508, piR-23230, piR-26525, piR-26527 and piR-28131. Conclusion: In our pilot profiling study of miRNA and piRNA in RCC we found altered expression patterns in tumor tissue and paired adjacent non-tumor renal parenchyma. We successfully detected some miRNAs (e.q. miR-155, miR-210, miR-200b) described as deregulated in accordance with previous studies. However, further validation on a larger set of patients is needed for elucidation of role miRNAs and piRNAs in molecular pathology of RCC.

Návaznosti

NT13514, projekt VaV

Název: Analýza mikroRNA v glioblastomových kmenových buňkách: predikce léčebné odpovědi a identifikace nových terapeutických cílů u pacientů s glioblastomem

NT13547, projekt VaV

Název: Studium mikroRNA a genů asociovaných s procesem epiteliálně-mezenchymální tranzice jako potenciálních markerů pro predikci rizika a časný záchyt metastazování u pacientů s renálním karcinomem

NT13549, projekt VaV

Název: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem

NT13860, projekt VaV

Název: Studium signální dráhy EGFR a expresních profilů mikroRNA v predikci odpovědi na cílenou anti-EGFR terapii u pacientů s kolorektálním karcinomem s nemutovanou variantou onkogenu KRAS

Citovat

ILIEV, Robert, Petra VYCHYTILOVÁ, Zuzana OŽANOVÁ, Silvia RYBECKÁ, Lenka RADOVÁ, Michal STANÍK, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK a Ondřej SLABÝ. DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS. In XL. brněnské onkologické dny a XXX. koncerence pro nelékařské zdravotnické pracovníky, Brno. 2016. ISSN 0862-495X.

@proceedings{1344247,
   author = {Iliev, Robert and Vychytilová, Petra and Ožanová, Zuzana and Rybecká, Silvia and Radová, Lenka and Staník, Michal and Doležel, Jan and Fedorko, Michal and Pacík, Dalibor and Slabý, Ondřej},
   booktitle = {XL. brněnské onkologické dny a XXX. koncerence pro nelékařské zdravotnické pracovníky, Brno},
   language = {eng},
   title = {DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS},
   year = {2016}
}

TY  - CONF
ID  - 1344247
AU  - Iliev, Robert - Vychytilová, Petra - Ožanová, Zuzana - Rybecká, Silvia - Radová, Lenka - Staník, Michal - Doležel, Jan - Fedorko, Michal - Pacík, Dalibor - Slabý, Ondřej
PY  - 2016
TI  - DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS
N2  - Introduction: MicroRNAs (miRNAs) are the class of small non-cod ing RNAs long 21– 25 nucleotides. They play an important role in regulation of transcription. They affect gene expres sion at post-transcriptional levels through binding to complementary mRNAs and mediate their degradation in RISC complex. Piwi-interacting RNAs (piRNAs) is newly discovered class of small non-cod ing RNA. They are single-stranded RNAs long 26– 31 nucleotides. They are involved in silencing of transposable elements and also participate in sequence-specific chromatin modifications. Deregulation of piRNAs was observed in kidney, bladder, gastric, breast, pancreatic and liver cancers. Patients and Methods: In our study we used the tumor tis sue and the paired adjacent non-tumor renal parenchyma of 12 patients (7 males and 5 females) with renal cell carcinoma (RCC) hospitalized in Masaryk Memorial Cancer Institute. RNA was isolated with mirVana™ miRNA Isolation Kit. For prepar ing RNA library was used TruSeq Small RNA Sample Preparation Kit from Il lumina and then the miSeq sequencing technology was used to detect small RNAs. Results: In our 12 paired samples of tumor tis sue and the paired adjacent non-tumor renal parenchyma we detected 283 miRNAs with > 1 read in at least 13 samples. 55 miRNAs were statistically significant different expressed (p < 0.05) in tumor tissue then in adjacent non-tumor parenchyma. MiRNAs with most significant altered expression (p < 0.01) were miR-129, miR-138, miR-142, miR-149, miR-154, miR-155, miR-200b, miR-210, miR-218, miR-340, miR-584, miR-885, miR-891a, miR-1270, miR-3690 and miR-7641. After piRNA sequences analysis we found 440 piRNAs with > 1 read in at least 13 samples. From these piRNAs were 38 piRNAs statistical ly signifi cant deregulated (p < 0.01). Most statisticaly significant altered expression levels were observed in piR-1207, piR-2107, piR-2155, piR-12487, piR-12488, piR-21508, piR-23230, piR-26525, piR-26527 and piR-28131. Conclusion: In our pilot profiling study of miRNA and piRNA in RCC we found altered expression patterns in tumor tissue and paired adjacent non-tumor renal parenchyma. We successfully detected some miRNAs (e.q. miR-155, miR-210, miR-200b) described as deregulated in accordance with previous studies. However, further validation on a larger set of patients is needed for elucidation of role miRNAs and piRNAs in molecular pathology of RCC.
ER  -

ILIEV, Robert, Petra VYCHYTILOVÁ, Zuzana OŽANOVÁ, Silvia RYBECKÁ, Lenka RADOVÁ, Michal STANÍK, Jan DOLEŽEL, Michal FEDORKO, Dalibor PACÍK a Ondřej SLABÝ. DETECTION OF ALTERED MIRNA AND PIRNA EXPRESSION LEVELS IN RENAL CELL CARCINOMA BY NGS. In \textit{XL. brněnské onkologické dny a XXX. koncerence pro nelékařské zdravotnické pracovníky, Brno}. 2016. ISSN~0862-495X.

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