Toxoplasma gondii and Neospora caninum in wild small mammals:
seroprevalence, DNA detection and genotyping

J 2016

Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK, Eva BÁRTOVÁ et. al.

Základní údaje

Originální název

Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping

Autoři

MACHAČOVÁ, Tereza (203 Česká republika, garant), Daniel AJZENBERG (250 Francie), Alena ŽÁKOVSKÁ (203 Česká republika, domácí), Kamil SEDLÁK (203 Česká republika) a Eva BÁRTOVÁ (203 Česká republika)

Vydání

Veterinary Parasitology, Elsevier Science, 2016, 0304-4017

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.356

Kód RIV

RIV/00216224:14310/16:00089871

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.vetpar.2016.04.018

UT WoS

000377317200015

Klíčová slova česky

Toxoplasmosis neosporosis hlodavci genotyp

Klíčová slova anglicky

Toxoplasmosis neosporosis rodents genotype Czech Republic

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 4. 2017 20:58, Ing. Andrea Mikešková

Anotace

V originále

Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002 – 2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5 percent) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay (percent inhibition value is more than 30 percent) in one A. flavicolis (0.4 percent). Three of 427 (0.7 percent) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. Three T. gondii isolates (two A. flavicollis and one A. sylvaticus) were genotyped by microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.

Citovat

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK a Eva BÁRTOVÁ. Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping. Veterinary Parasitology. Elsevier Science, 2016, roč. 223, June, s. 88-90. ISSN 0304-4017. Dostupné z: https://dx.doi.org/10.1016/j.vetpar.2016.04.018.

@article{1344738,
   author = {Machačová, Tereza and Ajzenberg, Daniel and Žákovská, Alena and Sedlák, Kamil and Bártová, Eva},
   article_number = {June},
   doi = {http://dx.doi.org/10.1016/j.vetpar.2016.04.018},
   keywords = {Toxoplasmosis neosporosis rodents genotype Czech Republic},
   language = {eng},
   issn = {0304-4017},
   journal = {Veterinary Parasitology},
   title = {Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping},
   volume = {223},
   year = {2016}
}

TY  - JOUR
ID  - 1344738
AU  - Machačová, Tereza - Ajzenberg, Daniel - Žákovská, Alena - Sedlák, Kamil - Bártová, Eva
PY  - 2016
TI  - Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping
JF  - Veterinary Parasitology
VL  - 223
IS  - June
SP  - 88-90
EP  - 88-90
PB  - Elsevier Science
SN  - 03044017
KW  - Toxoplasmosis neosporosis rodents genotype Czech Republic
N2  - Generally, rodents and other small mammals are considered as one of the sources of Toxoplasma gondii or Neospora caninum infection for cats and dogs as the definitive hosts of these two parasites, respectively. The aim of the study was to find out the prevalence of these two parasites in wild small mammals from the Czech Republic and to characterize T. gondii isolates by methods of molecular biology. A total of 621 wild small mammals were caught in the Czech Republic during years 2002 – 2014. Antibodies to T. gondii were detected by latex agglutination test in six (2.5 percent) of 240 small mammals (in two A. agrarius and four A. flavicollis). Antibodies to N. caninum were detected by commercially available competitive-inhibition enzyme-linked immunosorbent assay (percent inhibition value is more than 30 percent) in one A. flavicolis (0.4 percent). Three of 427 (0.7 percent) liver samples were positive for T. gondii by PCR while negative for N. caninum. All embryo samples (n = 102) were negative for both T. gondii and N. caninum. Three T. gondii isolates (two A. flavicollis and one A. sylvaticus) were genotyped by microsatellite markers and characterized as type II. To our knowledge, this is the first information about genetic characterization of T. gondii isolates in small mammals from Europe and the first detection of N. caninum antibodies in wild rodents from the Czech Republic.
ER  -

MACHAČOVÁ, Tereza, Daniel AJZENBERG, Alena ŽÁKOVSKÁ, Kamil SEDLÁK a Eva BÁRTOVÁ. Toxoplasma gondii and Neospora caninum in wild small mammals: seroprevalence, DNA detection and genotyping. \textit{Veterinary Parasitology}. Elsevier Science, 2016, roč.~223, June, s.~88-90. ISSN~0304-4017. Dostupné z: https://dx.doi.org/10.1016/j.vetpar.2016.04.018.

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