Electrochemical and spectral behaviour of miR-34a-5p

J 2016

Electrochemical and spectral behaviour of miR-34a-5p

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK, Libuše TRNKOVÁ et. al.

Základní údaje

Originální název

Electrochemical and spectral behaviour of miR-34a-5p

Autoři

VEČEŘOVÁ, Aneta (203 Česká republika, domácí), Kristýna HUDCOVÁ (203 Česká republika, domácí), Iveta PILAŘOVÁ (203 Česká republika, domácí), Michal MASAŘÍK (203 Česká republika, garant, domácí) a Libuše TRNKOVÁ (203 Česká republika, domácí)

Vydání

Monatshefte fur Chemie, Wien, Springer, 2016, 0026-9247

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10610 Biophysics

Stát vydavatele

Rakousko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.282

Kód RIV

RIV/00216224:14110/16:00089932

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1007/s00706-015-1600-y

UT WoS

000367521400014

Klíčová slova anglicky

miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 17:43, Mgr. Marie Šípková, DiS.

Anotace

V originále

MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA -> miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3-6. [GRAPHICS] .

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

LH13053, projekt VaV

Název: Chemická a elektrochemická analýza purinových derivátů a jejich komplexů s mědí (Akronym: CECAP)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Chemická a elektrochemická analýza purinových derivátů

Citovat

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK a Libuše TRNKOVÁ. Electrochemical and spectral behaviour of miR-34a-5p. Monatshefte fur Chemie. Wien: Springer, 2016, roč. 147, č. 1, s. 105-110. ISSN 0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-015-1600-y.

@article{1345275,
   author = {Večeřová, Aneta and Hudcová, Kristýna and Pilařová, Iveta and Masařík, Michal and Trnková, Libuše},
   article_location = {Wien},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s00706-015-1600-y},
   keywords = {miR-34a-5p; Human cancer; Voltammetry; Circular dichroic spectroscopy; Mercury electrode},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte fur Chemie},
   title = {Electrochemical and spectral behaviour of miR-34a-5p},
   url = {http://link.springer.com/article/10.1007%2Fs00706-015-1600-y},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1345275
AU  - Večeřová, Aneta - Hudcová, Kristýna - Pilařová, Iveta - Masařík, Michal - Trnková, Libuše
PY  - 2016
TI  - Electrochemical and spectral behaviour of miR-34a-5p
JF  - Monatshefte fur Chemie
VL  - 147
IS  - 1
SP  - 105-110
EP  - 105-110
PB  - Springer
SN  - 00269247
KW  - miR-34a-5p
KW  - Human cancer
KW  - Voltammetry
KW  - Circular dichroic spectroscopy
KW  - Mercury electrode
UR  - http://link.springer.com/article/10.1007%2Fs00706-015-1600-y
L2  - http://link.springer.com/article/10.1007%2Fs00706-015-1600-y
N2  - MicroRNAs are a family of small noncoding RNAs, regulating gene expression at the post-transcriptional level and playing a crucial role as potential biomarkers and targets for many types of cancer. Our attention was given to electrochemical and spectral studies of miR-34a-5p, related to head and neck and also prostate cancer. Voltammetric experiments on a hanging mercury drop electrode and circular dichroic spectroscopic experiments in buffer solutions were performed in the range of pH from 3 to 6 and from 3 to 8, respectively. The miRNA and its DNA sequential analogues (miDNA) provided interesting and significantly different results. A comparison of miRNA and both miDNA (U) and (T) showed the effect of substitution of ribose to deoxyribose, and structural diversity of nucleic acids was confirmed by both electrochemical and spectral methods. It emerged that a single substitution of ribose to deoxyribose (equivalent to miRNA -> miDNA (U)) leads on to the formation of a second guanine oxidation peak, and an RNA molecule in contrast to DNA analogues offers circular dichroic spectra of higher variability (indicating more terminal structures) and a more observable dependence on pH in the range of 3-6. [GRAPHICS] .
ER  -

VEČEŘOVÁ, Aneta, Kristýna HUDCOVÁ, Iveta PILAŘOVÁ, Michal MASAŘÍK a Libuše TRNKOVÁ. Electrochemical and spectral behaviour of miR-34a-5p. \textit{Monatshefte fur Chemie}. Wien: Springer, 2016, roč.~147, č.~1, s.~105-110. ISSN~0026-9247. Dostupné z: https://dx.doi.org/10.1007/s00706-015-1600-y.

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