Freeze-drying as suitable method to achieve ready-to-use yeast
biosensors for androgenic and estrogenic compounds

J 2016

Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds

JARQUE ORTIZ, Sergio, Michal BITTNER a Klára HILSCHEROVÁ

Základní údaje

Originální název

Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds

Autoři

JARQUE ORTIZ, Sergio (724 Španělsko, domácí), Michal BITTNER (203 Česká republika, domácí) a Klára HILSCHEROVÁ (203 Česká republika, garant, domácí)

Vydání

Chemosphere, OXFORD, Elsevier Science, 2016, 0045-6535

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30304 Public and environmental health

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.208

Kód RIV

RIV/00216224:14310/16:00089983

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.chemosphere.2016.01.038

UT WoS

000371099700024

Klíčová slova anglicky

Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 7. 2017 13:21, RNDr. Mgr. Michal Bittner, Ph.D.

Anotace

V originále

Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.

Návaznosti

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

JARQUE ORTIZ, Sergio, Michal BITTNER a Klára HILSCHEROVÁ. Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds. Chemosphere. OXFORD: Elsevier Science, 2016, roč. 148, April, s. 204-210. ISSN 0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2016.01.038.

@article{1345764,
   author = {Jarque Ortiz, Sergio and Bittner, Michal and Hilscherová, Klára},
   article_location = {OXFORD},
   article_number = {April},
   doi = {http://dx.doi.org/10.1016/j.chemosphere.2016.01.038},
   keywords = {Recombinant yeast assay; Freeze-drying; Estrogen; Androgen; Long-term; Ready-to-use},
   language = {eng},
   issn = {0045-6535},
   journal = {Chemosphere},
   title = {Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds},
   url = {http://www.sciencedirect.com/science/article/pii/S0045653516300376},
   volume = {148},
   year = {2016}
}

TY  - JOUR
ID  - 1345764
AU  - Jarque Ortiz, Sergio - Bittner, Michal - Hilscherová, Klára
PY  - 2016
TI  - Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds
JF  - Chemosphere
VL  - 148
IS  - April
SP  - 204-210
EP  - 204-210
PB  - Elsevier Science
SN  - 00456535
KW  - Recombinant yeast assay
KW  - Freeze-drying
KW  - Estrogen
KW  - Androgen
KW  - Long-term
KW  - Ready-to-use
UR  - http://www.sciencedirect.com/science/article/pii/S0045653516300376
L2  - http://www.sciencedirect.com/science/article/pii/S0045653516300376
N2  - Recombinant yeast assays (RYAS) have been proved to be a suitable tool for the fast screening of compounds with endocrine disrupting activities. However, ready-to-use versions more accessible to less equipped laboratories and field studies are scarce and far from optimal throughputs. Here, we have applied freeze-drying technology to optimize RYA for the fast assessment of environmental compounds with estrogenic and androgenic potencies. The effects of different cryoprotectants, initial optical density and long-term storage were evaluated. The study included detailed characterization of sensitivity, robustness and reproducibility of the new ready-to-use versions, as well as comparison with the standard assays. Freeze-dried RYAS showed similar dose-responses curves to their homolog standard assays, with Lowest Observed Effect Concentration (LOEC) and Median effective Concentration (EC50) of 1 nM and 7.5 nM for testosterone, and 0.05 nM and 0.5 nM for 17 beta-estradiol, respectively. Freeze-dried cells stored at 4 degrees C retained maximum sensitivity up to 2 months, while cells stored at -18 degrees C showed no decrease in sensitivity throughout the study (10 months). This ready-to-use RYA is easily accessible and may be potentially used for on-site applications.
ER  -

JARQUE ORTIZ, Sergio, Michal BITTNER a Klára HILSCHEROVÁ. Freeze-drying as suitable method to achieve ready-to-use yeast biosensors for androgenic and estrogenic compounds. \textit{Chemosphere}. OXFORD: Elsevier Science, 2016, roč.~148, April, s.~204-210. ISSN~0045-6535. Dostupné z: https://dx.doi.org/10.1016/j.chemosphere.2016.01.038.

Podrobný výpis o publikaci