Study of DNA immobilization on mica surface by atomic force
microscopy

J 2016

Study of DNA immobilization on mica surface by atomic force microscopy

HORŇÁKOVÁ, Veronika, Jan PŘIBYL and Petr SKLÁDAL

Basic information

Original name

Study of DNA immobilization on mica surface by atomic force microscopy

Authors

HORŇÁKOVÁ, Veronika (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution) and Petr SKLÁDAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Monatshefte fur Chemie, Wien, Springer Wien, 2016, 0026-9247

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.282

RIV identification code

RIV/00216224:14740/16:00090063

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1007/s00706-016-1695-9

UT WoS

000374172200004

Keywords in English

Atomic force microscopy; DNA; Nanostructures; Silanization; Cations; Ethanolamine

Abstract

V originále

Since its discovery, atomic force microscopy (AFM) is widely used to study biological objects and materials, including cells, proteins, and nucleic acids. AFM measurements are carried out in the air as well as in liquid with a very high resolution, even more complex bioprocesses can be monitored in situ under physiological conditions. Successful imaging of DNA molecules on the flat supporting surface typically requires appropriate treatment of mica. The original surface charge of mica is the same as of DNA, i.e. negative. Accordingly, immobilization using bivalent cations (Mg2+, Ni2+, and Co2+), deposition of ethanolamine, and mica surface silanization with alkoxysiloxane derivatives were reported to achieve an optimal concentration and surface arrangement of DNA molecules. Vapours of alkoxysiloxane derivatives led to uniform negatively charged mica surface and it was found that higher ionic radius causes a weaker bond. A better quality and sharper images of DNA molecules were achieved by adjusting the correct real amplitude of the cantilever. This amplitude should correspond with the expected size of the target objects-DNA molecules in the x-y plane of the image. The length of the observed DNA molecules was 1000 bp and the planar width of DNA was 7.8 nm (in reality 3 nm). The AFM spectroscopic mode was particularly useful.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

Citovat

HORŇÁKOVÁ, Veronika, Jan PŘIBYL and Petr SKLÁDAL. Study of DNA immobilization on mica surface by atomic force microscopy. Monatshefte fur Chemie. Wien: Springer Wien, 2016, vol. 147, No 5, p. 865-871. ISSN 0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1695-9.

@article{1346400,
   author = {Horňáková, Veronika and Přibyl, Jan and Skládal, Petr},
   article_location = {Wien},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s00706-016-1695-9},
   keywords = {Atomic force microscopy; DNA; Nanostructures; Silanization; Cations; Ethanolamine},
   language = {eng},
   issn = {0026-9247},
   journal = {Monatshefte fur Chemie},
   title = {Study of DNA immobilization on mica surface by atomic force microscopy},
   url = {http://link.springer.com/article/10.1007%2Fs00706-016-1695-9},
   volume = {147},
   year = {2016}
}

TY  - JOUR
ID  - 1346400
AU  - Horňáková, Veronika - Přibyl, Jan - Skládal, Petr
PY  - 2016
TI  - Study of DNA immobilization on mica surface by atomic force microscopy
JF  - Monatshefte fur Chemie
VL  - 147
IS  - 5
SP  - 865-871
EP  - 865-871
PB  - Springer Wien
SN  - 00269247
KW  - Atomic force microscopy
KW  - DNA
KW  - Nanostructures
KW  - Silanization
KW  - Cations
KW  - Ethanolamine
UR  - http://link.springer.com/article/10.1007%2Fs00706-016-1695-9
L2  - http://link.springer.com/article/10.1007%2Fs00706-016-1695-9
N2  - Since its discovery, atomic force microscopy (AFM) is widely used to study biological objects and materials, including cells, proteins, and nucleic acids. AFM measurements are carried out in the air as well as in liquid with a very high resolution, even more complex bioprocesses can be monitored in situ under physiological conditions. Successful imaging of DNA molecules on the flat supporting surface typically requires appropriate treatment of mica. The original surface charge of mica is the same as of DNA, i.e. negative. Accordingly, immobilization using bivalent cations (Mg2+, Ni2+, and Co2+), deposition of ethanolamine, and mica surface silanization with alkoxysiloxane derivatives were reported to achieve an optimal concentration and surface arrangement of DNA molecules. Vapours of alkoxysiloxane derivatives led to uniform negatively charged mica surface and it was found that higher ionic radius causes a weaker bond. A better quality and sharper images of DNA molecules were achieved by adjusting the correct real amplitude of the cantilever. This amplitude should correspond with the expected size of the target objects-DNA molecules in the x-y plane of the image. The length of the observed DNA molecules was 1000 bp and the planar width of DNA was 7.8 nm (in reality 3 nm). The AFM spectroscopic mode was particularly useful.
ER  -

HORŇÁKOVÁ, Veronika, Jan PŘIBYL and Petr SKLÁDAL. Study of DNA immobilization on mica surface by atomic force microscopy. \textit{Monatshefte fur Chemie}. Wien: Springer Wien, 2016, vol.~147, No~5, p.~865-871. ISSN~0026-9247. Available from: https://dx.doi.org/10.1007/s00706-016-1695-9.

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