ŠVEC, Pavel, Jozef KOVÁČIK, Josef HEDBAVNÝ, Petr BABULA, Gabriela ROTKOVÁ and Bořivoj KLEJDUS. Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda. Water, Air and Soil Pollution. Dordrecht: Springer, 2016, vol. 227, No 5, p. 1-9. ISSN 0049-6979. Available from: https://dx.doi.org/10.1007/s11270-016-2859-2.
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Basic information
Original name Impact of Anions, Cations, and pH on Manganese Accumulation and Toxicity in the Green Alga Scenedesmus quadricauda
Authors ŠVEC, Pavel (203 Czech Republic), Jozef KOVÁČIK (203 Czech Republic), Josef HEDBAVNÝ (203 Czech Republic), Petr BABULA (203 Czech Republic, guarantor, belonging to the institution), Gabriela ROTKOVÁ (203 Czech Republic, belonging to the institution) and Bořivoj KLEJDUS (203 Czech Republic).
Edition Water, Air and Soil Pollution, Dordrecht, Springer, 2016, 0049-6979.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30105 Physiology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 1.702
RIV identification code RIV/00216224:14110/16:00090098
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1007/s11270-016-2859-2
UT WoS 000376276100032
Keywords in English Algae; Antioxidants; Bioaccumulation; Metals; Oxidative stress
Tags EL OK, podil
Tags International impact, Reviewed
Changed by Changed by: Ing. Mgr. Věra Pospíšilíková, učo 9005. Changed: 25/8/2016 08:43.
Abstract
Accumulation of divalent manganese (Mn) and its toxicity in the green alga Scenedesmus quadricauda was studied at circumneutral pH (6.5). A comparison of two applied concentrations (10 or 100 mu M) of MnCl2, MnSO4, and Mn(NO3)(2) indicated that mainly sulfate evoked higher Mn accumulation. On the other hand, nitrate rather depleted antioxidative enzyme activities (APX, CAT, SOD), leading to an increase in ROS formation as proven by fluorescence microscopy. Subsequent experiments revealed that increase in pH (from 4.5 to 9.5) increased also Mn content but typically depleted amounts of reduced glutathione and phytochelatin 2. We also measured the size of particles formed from the manganese salts at pH 9.5. Competitive experiment between Ca/K salts (CaCl2, CaSO4, Ca(NO3)(2), KCl, K2SO4, KNO3) and Mn (as Mn sulfate) showed a negative relation between Ca and Mn amount but KNO3 stimulated Mn accumulation. Microscopy revealed that mainly K salts elevated plasma membrane damage (Acridine orange staining). Data indicate that not only pH but also accompanying anion affects Mn accumulation and that Ca salts may affect Mn toxicity.
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