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@article{1347090,
author = {Zedek, František and Veselý, Pavel and Horová, Lucie and Bureš, Petr},
article_location = {LONDON},
article_number = {June},
doi = {http://dx.doi.org/10.1038/srep27161},
keywords = {endopolyploidy; flow cytometry; holocentric chromosomes},
language = {eng},
issn = {2045-2322},
journal = {Scientific Reports},
title = {Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants},
url = {http://www.nature.com/articles/srep27161},
volume = {6},
year = {2016}
}
TY - JOUR
ID - 1347090
AU - Zedek, František - Veselý, Pavel - Horová, Lucie - Bureš, Petr
PY - 2016
TI - Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants
JF - Scientific Reports
VL - 6
IS - June
SP - "nestrankovano"
EP - "nestrankovano"
PB - NATURE PUBLISHING GROUP
SN - 20452322
KW - endopolyploidy
KW - flow cytometry
KW - holocentric chromosomes
UR - http://www.nature.com/articles/srep27161
N2 - Two chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally. In monocentric species, unequal distribution should generate chimeras of cells with different nuclear DNA content. We investigated whether such differences in monocentric species are detectable by flow cytometry (FCM) as (i) a decreased nuclear DNA content and (ii) an increased coefficient of variance (CV) of the G1 peak after gamma radiation-induced fragmentation. We compared 13 monocentric and 9 holocentric plant species. Unexpectedly, monocentrics and holocentrics did not differ with respect to parameters (i) and (ii) in their response to gamma irradiation. However, we found that the proportion of G2 nuclei was highly elevated in monocentrics after irradiation, while holocentrics were negligibly affected. Therefore, we hypothesize that DNA-damaging agents induce cell cycle arrest leading to endopolyploidy only in monocentric and not (or to much lesser extent) in holocentric plants. While current microscope-dependent methods for holocentrism detection are unreliable for small and numerous chromosomes, which are common in holocentrics, FCM can use somatic nuclei. Thus, FCM may be a rapid and reliable method of high-throughput screening for holocentric candidates across plant phylogeny.
ER -
ZEDEK, František, Pavel VESELÝ, Lucie HOROVÁ a Petr BUREŠ. Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2016, roč.~6, June, s.~''nestrankovano'', 7 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep27161.
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