Flow cytometry may allow microscope-independent detection of
holocentric chromosomes in plants

J 2016

Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants

ZEDEK, František, Pavel VESELÝ, Lucie HOROVÁ a Petr BUREŠ

Základní údaje

Originální název

Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants

Název česky

Průtoková cytometrie může sloužit jako na mikroskopických technikách nezávislá metoda detekce holocentrických chromosomů u rostlin

Autoři

ZEDEK, František (203 Česká republika, domácí), Pavel VESELÝ (203 Česká republika, domácí), Lucie HOROVÁ (203 Česká republika, domácí) a Petr BUREŠ (203 Česká republika, garant, domácí)

Vydání

Scientific Reports, LONDON, NATURE PUBLISHING GROUP, 2016, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.259

Kód RIV

RIV/00216224:14310/16:00087971

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1038/srep27161

UT WoS

000377001500001

Klíčová slova česky

endopolyploidie; průtoková cytometrie; holocentrické chromosomy

Klíčová slova anglicky

endopolyploidy; flow cytometry; holocentric chromosomes

Štítky

AKR

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 6. 3. 2018 16:18, prof. RNDr. Petr Bureš, Ph.D.

Anotace

V originále

Two chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally. In monocentric species, unequal distribution should generate chimeras of cells with different nuclear DNA content. We investigated whether such differences in monocentric species are detectable by flow cytometry (FCM) as (i) a decreased nuclear DNA content and (ii) an increased coefficient of variance (CV) of the G1 peak after gamma radiation-induced fragmentation. We compared 13 monocentric and 9 holocentric plant species. Unexpectedly, monocentrics and holocentrics did not differ with respect to parameters (i) and (ii) in their response to gamma irradiation. However, we found that the proportion of G2 nuclei was highly elevated in monocentrics after irradiation, while holocentrics were negligibly affected. Therefore, we hypothesize that DNA-damaging agents induce cell cycle arrest leading to endopolyploidy only in monocentric and not (or to much lesser extent) in holocentric plants. While current microscope-dependent methods for holocentrism detection are unreliable for small and numerous chromosomes, which are common in holocentrics, FCM can use somatic nuclei. Thus, FCM may be a rapid and reliable method of high-throughput screening for holocentric candidates across plant phylogeny.

Návaznosti

GA13-29362S, projekt VaV

Název: Evoluce holocentrických chromosomů (Akronym: EvoHolo)

Investor: Grantová agentura ČR, Evoluce holocentrických chromosomů

Citovat

ZEDEK, František, Pavel VESELÝ, Lucie HOROVÁ a Petr BUREŠ. Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants. Scientific Reports. LONDON: NATURE PUBLISHING GROUP, 2016, roč. 6, June, s. "nestrankovano", 7 s. ISSN 2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep27161.

@article{1347090,
   author = {Zedek, František and Veselý, Pavel and Horová, Lucie and Bureš, Petr},
   article_location = {LONDON},
   article_number = {June},
   doi = {http://dx.doi.org/10.1038/srep27161},
   keywords = {endopolyploidy; flow cytometry; holocentric chromosomes},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific Reports},
   title = {Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants},
   url = {http://www.nature.com/articles/srep27161},
   volume = {6},
   year = {2016}
}

TY  - JOUR
ID  - 1347090
AU  - Zedek, František - Veselý, Pavel - Horová, Lucie - Bureš, Petr
PY  - 2016
TI  - Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants
JF  - Scientific Reports
VL  - 6
IS  - June
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - endopolyploidy
KW  - flow cytometry
KW  - holocentric chromosomes
UR  - http://www.nature.com/articles/srep27161
N2  - Two chromosomal structures, known as monocentric and holocentric chromosomes, have evolved in eukaryotes. Acentric fragments of monocentric chromosomes are unequally distributed to daughter cells and/or lost, while holocentric fragments are inherited normally. In monocentric species, unequal distribution should generate chimeras of cells with different nuclear DNA content. We investigated whether such differences in monocentric species are detectable by flow cytometry (FCM) as (i) a decreased nuclear DNA content and (ii) an increased coefficient of variance (CV) of the G1 peak after gamma radiation-induced fragmentation. We compared 13 monocentric and 9 holocentric plant species. Unexpectedly, monocentrics and holocentrics did not differ with respect to parameters (i) and (ii) in their response to gamma irradiation. However, we found that the proportion of G2 nuclei was highly elevated in monocentrics after irradiation, while holocentrics were negligibly affected. Therefore, we hypothesize that DNA-damaging agents induce cell cycle arrest leading to endopolyploidy only in monocentric and not (or to much lesser extent) in holocentric plants. While current microscope-dependent methods for holocentrism detection are unreliable for small and numerous chromosomes, which are common in holocentrics, FCM can use somatic nuclei. Thus, FCM may be a rapid and reliable method of high-throughput screening for holocentric candidates across plant phylogeny.
ER  -

ZEDEK, František, Pavel VESELÝ, Lucie HOROVÁ a Petr BUREŠ. Flow cytometry may allow microscope-independent detection of holocentric chromosomes in plants. \textit{Scientific Reports}. LONDON: NATURE PUBLISHING GROUP, 2016, roč.~6, June, s.~''nestrankovano'', 7 s. ISSN~2045-2322. Dostupné z: https://dx.doi.org/10.1038/srep27161.

Podrobný výpis o publikaci