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@proceedings{1347260,
author = {Reslová, Nikol and Kašný, Martin and Slaný, Michal and Monteiro, Silvia and Santos, Ricardo},
booktitle = {XV. Tomáškovy dny mladých mikrobiologů},
keywords = {real-time PCR; digital PCR; diagnostics of foodborne parasites; giardia intestinalis; toxoplasma gondii},
language = {cze},
isbn = {978-80-210-8255-7},
title = {Real-time PCR a digital PCR v diagnostice parazitů v potravinách},
year = {2016}
}
TY - CONF
ID - 1347260
AU - Reslová, Nikol - Kašný, Martin - Slaný, Michal - Monteiro, Silvia - Santos, Ricardo
PY - 2016
TI - Real-time PCR a digital PCR v diagnostice parazitů v potravinách
SN - 9788021082557
KW - real-time PCR
KW - digital PCR
KW - diagnostics of foodborne parasites
KW - giardia intestinalis
KW - toxoplasma gondii
N2 - In recent years, the increase of reported outbreaks causing human-diseases associated with food-borne parasitic infections (uni/multi-cellular) arising from meat consumption has been recorded. This trend could be affected e.g. by the changes in farming management towards bio-production, globalization of food market, global climate change and also by the meat processing. This situation is consequently inducing the need for improvement of the diagnostic applications. Our work is focused on development of a reliable comprehensive molecular diagnostic method useful for rapid control of meat products. We adopted high sensitive multiplex oligonucleotide ligation-PCR assay - MOL-PCR, enabling direct and simultaneous detection of multiple nucleic acid signatures of parasites from complex samples and xMAP technology representing a novel detection platform based on magnetic microspheres. Visualization of the products is then realized via qualitative and quantitative MAGPIX instrumentation. Up to this date, the specific molecular probes allowing the detection and capturing of targeted DNA originating from two parasitic worms - Trichinella spiralis (nematode; partial sequence of 18S rRNA gene) and Taenia saginata (cestode; partial sequence of mitochondrial COX1 gene) were designed. Reaction conditions of MOL-PCR were optimized for simultaneous duplex assay. Currently, the calibration of MAGPIX system is in process.
ER -
RESLOVÁ, Nikol, Martin KAŠNÝ, Michal SLANÝ, Silvia MONTEIRO a Ricardo SANTOS. Real-time PCR a digital PCR v diagnostice parazitů v potravinách. In \textit{XV. Tomáškovy dny mladých mikrobiologů}. 2016. ISBN~978-80-210-8255-7.
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