Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in
Dairy Cows

J 2016

Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows

KAŠPAROVSKÁ, Jitka, Martina ZAPLETALOVÁ, Kateřina DADÁKOVÁ, Ludmila KŘÍŽOVÁ, Sylvie HADROVÁ et. al.

Základní údaje

Originální název

Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows

Autoři

KAŠPAROVSKÁ, Jitka (203 Česká republika, domácí), Martina ZAPLETALOVÁ (203 Česká republika, domácí), Kateřina DADÁKOVÁ (203 Česká republika, domácí), Ludmila KŘÍŽOVÁ (203 Česká republika, domácí), Sylvie HADROVÁ (203 Česká republika, domácí), Matej LEXA (703 Slovensko, domácí), Jan LOCHMAN (203 Česká republika, domácí) a Tomáš KAŠPAROVSKÝ (203 Česká republika, domácí)

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14310/16:00087977

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0154642

UT WoS

000375211700120

Klíčová slova anglicky

dairy cow; soybean extract; equol; carry-over rate; rumen; bacterial phylogenetics

Štítky

AKR, rivok

Změněno: 5. 4. 2017 10:51, Ing. Andrea Mikešková

Anotace

V originále

In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 mu g/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 mu g/L in the CTRL group to 186.30 mu g/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings.

Návaznosti

GA13-34134S, projekt VaV

Název: Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce

Investor: Grantová agentura ČR, Metabolizmus izoflavonů v bachoru se zaměřením na obsah equolu v kravském mléce

Citovat

KAŠPAROVSKÁ, Jitka, Martina ZAPLETALOVÁ, Kateřina DADÁKOVÁ, Ludmila KŘÍŽOVÁ, Sylvie HADROVÁ, Matej LEXA, Jan LOCHMAN a Tomáš KAŠPAROVSKÝ. Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 4, s. "nestrankovano", 17 s. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0154642.

@article{1347272,
   author = {Kašparovská, Jitka and Zapletalová, Martina and Dadáková, Kateřina and Křížová, Ludmila and Hadrová, Sylvie and Lexa, Matej and Lochman, Jan and Kašparovský, Tomáš},
   article_location = {San Francisco},
   article_number = {4},
   doi = {http://dx.doi.org/10.1371/journal.pone.0154642},
   keywords = {dairy cow; soybean extract; equol; carry-over rate; rumen; bacterial phylogenetics},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1347272
AU  - Kašparovská, Jitka - Zapletalová, Martina - Dadáková, Kateřina - Křížová, Ludmila - Hadrová, Sylvie - Lexa, Matej - Lochman, Jan - Kašparovský, Tomáš
PY  - 2016
TI  - Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows
JF  - Plos one
VL  - 11
IS  - 4
SP  - "nestrankovano"
EP  - "nestrankovano"
PB  - Public Library of Science
SN  - 19326203
KW  - dairy cow
KW  - soybean extract
KW  - equol
KW  - carry-over rate
KW  - rumen
KW  - bacterial phylogenetics
N2  - In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 mu g/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 mu g/L in the CTRL group to 186.30 mu g/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings.
ER  -

KAŠPAROVSKÁ, Jitka, Martina ZAPLETALOVÁ, Kateřina DADÁKOVÁ, Ludmila KŘÍŽOVÁ, Sylvie HADROVÁ, Matej LEXA, Jan LOCHMAN a Tomáš KAŠPAROVSKÝ. Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~4, s.~''nestrankovano'', 17 s. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0154642.

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