KAŠPAROVSKÁ, Jitka, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Kateřina DADÁKOVÁ a Tomáš KAŠPAROVSKÝ. Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF). Journal of Chromatographic Science. Oxford University Press, 2016, roč. 54, č. 6, s. 997-1003. ISSN 0021-9665. Dostupné z: https://dx.doi.org/10.1093/chromsci/bmw033. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1347276, author = {Kašparovská, Jitka and Křížová, Ludmila and Lochman, Jan and Dadáková, Kateřina and Kašparovský, Tomáš}, article_number = {6}, doi = {http://dx.doi.org/10.1093/chromsci/bmw033}, keywords = {Isoflavones; equol; rumen fluid; milk; HPLC-MS}, language = {eng}, issn = {0021-9665}, journal = {Journal of Chromatographic Science}, title = {Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF).}, volume = {54}, year = {2016} }
TY - JOUR ID - 1347276 AU - Kašparovská, Jitka - Křížová, Ludmila - Lochman, Jan - Dadáková, Kateřina - Kašparovský, Tomáš PY - 2016 TI - Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF). JF - Journal of Chromatographic Science VL - 54 IS - 6 SP - 997-1003 EP - 997-1003 PB - Oxford University Press SN - 00219665 KW - Isoflavones KW - equol KW - rumen fluid KW - milk KW - HPLC-MS N2 - Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC–MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500–9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9–5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid. ER -
KAŠPAROVSKÁ, Jitka, Ludmila KŘÍŽOVÁ, Jan LOCHMAN, Kateřina DADÁKOVÁ a Tomáš KAŠPAROVSKÝ. Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC–MS-(TOF). \textit{Journal of Chromatographic Science}. Oxford University Press, 2016, roč.~54, č.~6, s.~997-1003. ISSN~0021-9665. Dostupné z: https://dx.doi.org/10.1093/chromsci/bmw033.
|