Development and validation of fast and simple HPLC for the
determination of uric acid, xanthine and hypoxanthine in human
plasma and serum

a 2016

Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum

PLESKAČOVÁ, Anna, Josef TOMANDL, Stanislav BREJCHA, Lukáš PÁCAL, Kateřina KAŇKOVÁ et. al.

Základní údaje

Originální název

Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum

Autoři

PLESKAČOVÁ, Anna, Josef TOMANDL, Stanislav BREJCHA, Lukáš PÁCAL a Kateřina KAŇKOVÁ

Vydání

Advances in Chromatography and Electrophoresis & Chiranal 2016, 2016

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

ISBN

978-80-244-4961-6

Klíčová slova česky

HPLC, kyselina močová, xantin, hypoxantin

Klíčová slova anglicky

HPLC, uric acid, xanthine, hypoxanthine

Příznaky

Mezinárodní význam

Změněno: 13. 6. 2016 10:36, Mgr. Anna Kolouchová, Ph.D.

Anotace

V originále

Uric acid (UA) is a terminal metabolite of purine metabolism generated from xanthine (X) and hypoxanthine (HX) by the action of xanthine oxidoreductase (XOR) as a rate limiting enzyme. An increase of XOR activity has been reported under several pathophysiological conditions [1] and elevated UA and X levels are important risk factors of several diseases including those related to lifestyle [2]. On the other hand UA is considered as an important antioxidant in vivo [3]. UA is usually determined in serum using an enzyme uricase in clinical chemistry laboratory. There are several disadvantages of this commonly used enzyme method, firstly it does not provide data on X and HX levels reflecting the activity of XOR, secondly hyperxanthinemia may result in false positive results and thirdly uricase requires bivalent ions and thus this method is not appropriate for EDTA-plasma samples. The aim of this study was to optimize an HPLC determination of UA, X and HX convenient for both human EDTA-plasma and serum.

Návaznosti

MUNI/A/1056/2015, interní kód MU

Název: Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění (Akronym: bichepat)

Investor: Masarykova univerzita, Příspěvek chemických a biochemických metodik ke studiu molekulární podstaty vybraných patologických stavů a onemocnění, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

PLESKAČOVÁ, Anna, Josef TOMANDL, Stanislav BREJCHA, Lukáš PÁCAL a Kateřina KAŇKOVÁ. Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum. In Advances in Chromatography and Electrophoresis & Chiranal 2016. 2016. ISBN 978-80-244-4961-6.

@proceedings{1347531,
   author = {Pleskačová, Anna and Tomandl, Josef and Brejcha, Stanislav and Pácal, Lukáš and Kaňková, Kateřina},
   booktitle = {Advances in Chromatography and Electrophoresis & Chiranal 2016},
   keywords = {HPLC, uric acid, xanthine, hypoxanthine},
   isbn = {978-80-244-4961-6},
   title = {Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum},
   year = {2016}
}

TY  - CONF
ID  - 1347531
AU  - Pleskačová, Anna - Tomandl, Josef - Brejcha, Stanislav - Pácal, Lukáš - Kaňková, Kateřina
PY  - 2016
TI  - Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum
SN  - 9788024449616
KW  - HPLC, uric acid, xanthine, hypoxanthine
N2  - Uric acid (UA) is a terminal metabolite of purine metabolism generated from xanthine (X) and hypoxanthine (HX) by the action of xanthine oxidoreductase (XOR) as a rate limiting enzyme. An increase of XOR activity has been reported under several pathophysiological conditions [1] and elevated UA and X levels are important risk factors of several diseases including those related to lifestyle [2]. On the other hand UA is considered as an important antioxidant in vivo [3]. UA is usually determined in serum using an enzyme uricase in clinical chemistry laboratory. There are several disadvantages of this commonly used enzyme method, firstly it does not provide data on X and HX levels reflecting the activity of XOR, secondly hyperxanthinemia may result in false positive results and thirdly uricase requires bivalent ions and thus this method is not appropriate for EDTA-plasma samples. The aim of this study was to optimize an HPLC determination of UA, X and HX convenient for both human EDTA-plasma and serum.
ER  -

PLESKAČOVÁ, Anna, Josef TOMANDL, Stanislav BREJCHA, Lukáš PÁCAL a Kateřina KAŇKOVÁ. Development and validation of fast and simple HPLC for the determination of uric acid, xanthine and hypoxanthine in human plasma and serum. In \textit{Advances in Chromatography and Electrophoresis \&{} Chiranal 2016}. 2016. ISBN~978-80-244-4961-6.

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