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@proceedings{1347612,
author = {Kolářová, Lenka and Kučera, Lukáš and Prokeš, Lubomír and Vaňhara, Petr and Hampl, Aleš and Havel, Josef},
booktitle = {In International conference on nanotechnology and nanosciences NN16},
keywords = {Mass spectrometry; gold nanoparticles; calibration},
language = {eng},
title = {Nano-gold in time-of-flight mass spectrometry of biological samples},
year = {2016}
}
TY - CONF
ID - 1347612
AU - Kolářová, Lenka - Kučera, Lukáš - Prokeš, Lubomír - Vaňhara, Petr - Hampl, Aleš - Havel, Josef
PY - 2016
TI - Nano-gold in time-of-flight mass spectrometry of biological samples
KW - Mass spectrometry
KW - gold nanoparticles
KW - calibration
N2 - The use of gold nano-particles (Au-NP) in science and technology is permanently increasing. Due to their unique physico-chemical properties, Au-NP are highly suitable for mass spectrometry (MS) of complex bio-molecules or intact cells and tissues as both calibrants and matrices. Au-NP are generally produced mostly by reduction of Au (III) with a redox agent. In the current work we demonstrate and discuss our recent results on use of Au-NP in MS of cells and tissues. 1) Internal/external calibration in MS: precise calibration in MS is an essential prerequisite of standard measurements. The use of Au clusters generated from various Au-NP provides a reliable tool for calibration in time-of-flight MS. The Au is monoisotopic, thus the determination of accurate mass is straightforward and fast compared to common calibration standards like peptides/proteins etc. 2) Use of Au-NP as matrix: the Au-NP (e.g. in the form of flower-like Au-NP) enhance the ionization of complex biomolecules. This enhancement is presumably due to SALDI (Surface Assisted Laser Desorption Ionization) or NALDI (Nano-particles Assisted LDI) effects. Even the combination of classical MALDI matrix with Au-NP offers significant advantages as it was recently reported [1]. 3) Au-NP in MS analysis of intact cells: gold is highly biocompatible; Au-NP can be easily absorbed by endocytosis to cell cytoplasm and increase the signal intensity of peptides and proteins during MS analysis. 4) Tissue imaging: similarly to Au-NP-mediated intact-cell MS, treatment of tissue sections with Au-NP-enriched matrices positively affects the overall quality of mass spectra and improves the tissue MS analysis. Concluding, the use of Au-NP brings new and exciting possibilities in MS of biomolecules.
ER -
KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Lubomír PROKEŠ, Petr VAŇHARA, Aleš HAMPL a Josef HAVEL. Nano-gold in time-of-flight mass spectrometry of biological samples. In \textit{In International conference on nanotechnology and nanosciences NN16}. 2016.
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