Nano-gold in time-of-flight mass spectrometry of biological
samples

a 2016

Nano-gold in time-of-flight mass spectrometry of biological samples

KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Lubomír PROKEŠ, Petr VAŇHARA, Aleš HAMPL et. al.

Základní údaje

Originální název

Nano-gold in time-of-flight mass spectrometry of biological samples

Autoři

KOLÁŘOVÁ, Lenka (203 Česká republika, domácí), Lukáš KUČERA (203 Česká republika, domácí), Lubomír PROKEŠ (203 Česká republika, domácí), Petr VAŇHARA (203 Česká republika, domácí), Aleš HAMPL (203 Česká republika, domácí) a Josef HAVEL (203 Česká republika, garant, domácí)

Vydání

In International conference on nanotechnology and nanosciences NN16, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10406 Analytical chemistry

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/16:00095853

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Mass spectrometry; gold nanoparticles; calibration

Změněno: 13. 3. 2018 14:53, Mgr. Lenka Kolářová, Ph.D.

Anotace

V originále

The use of gold nano-particles (Au-NP) in science and technology is permanently increasing. Due to their unique physico-chemical properties, Au-NP are highly suitable for mass spectrometry (MS) of complex bio-molecules or intact cells and tissues as both calibrants and matrices. Au-NP are generally produced mostly by reduction of Au (III) with a redox agent. In the current work we demonstrate and discuss our recent results on use of Au-NP in MS of cells and tissues. 1) Internal/external calibration in MS: precise calibration in MS is an essential prerequisite of standard measurements. The use of Au clusters generated from various Au-NP provides a reliable tool for calibration in time-of-flight MS. The Au is monoisotopic, thus the determination of accurate mass is straightforward and fast compared to common calibration standards like peptides/proteins etc. 2) Use of Au-NP as matrix: the Au-NP (e.g. in the form of flower-like Au-NP) enhance the ionization of complex biomolecules. This enhancement is presumably due to SALDI (Surface Assisted Laser Desorption Ionization) or NALDI (Nano-particles Assisted LDI) effects. Even the combination of classical MALDI matrix with Au-NP offers significant advantages as it was recently reported [1]. 3) Au-NP in MS analysis of intact cells: gold is highly biocompatible; Au-NP can be easily absorbed by endocytosis to cell cytoplasm and increase the signal intensity of peptides and proteins during MS analysis. 4) Tissue imaging: similarly to Au-NP-mediated intact-cell MS, treatment of tissue sections with Au-NP-enriched matrices positively affects the overall quality of mass spectra and improves the tissue MS analysis. Concluding, the use of Au-NP brings new and exciting possibilities in MS of biomolecules.

Citovat

KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Lubomír PROKEŠ, Petr VAŇHARA, Aleš HAMPL a Josef HAVEL. Nano-gold in time-of-flight mass spectrometry of biological samples. In In International conference on nanotechnology and nanosciences NN16. 2016.

@proceedings{1347612,
   author = {Kolářová, Lenka and Kučera, Lukáš and Prokeš, Lubomír and Vaňhara, Petr and Hampl, Aleš and Havel, Josef},
   booktitle = {In International conference on nanotechnology and nanosciences NN16},
   keywords = {Mass spectrometry; gold nanoparticles; calibration},
   language = {eng},
   title = {Nano-gold in time-of-flight mass spectrometry of biological samples},
   year = {2016}
}

TY  - CONF
ID  - 1347612
AU  - Kolářová, Lenka - Kučera, Lukáš - Prokeš, Lubomír - Vaňhara, Petr - Hampl, Aleš - Havel, Josef
PY  - 2016
TI  - Nano-gold in time-of-flight mass spectrometry of biological samples
KW  - Mass spectrometry
KW  - gold nanoparticles
KW  - calibration
N2  - The use of gold nano-particles (Au-NP) in science and technology is permanently increasing. Due to their unique physico-chemical properties, Au-NP are highly suitable for mass spectrometry (MS) of complex bio-molecules or intact cells and tissues as both calibrants and matrices. Au-NP are generally produced mostly by reduction of Au (III) with a redox agent. In the current work we demonstrate and discuss our recent results on use of Au-NP in MS of cells and tissues. 1) Internal/external calibration in MS: precise calibration in MS is an essential prerequisite of standard measurements. The use of Au clusters generated from various Au-NP provides a reliable tool for calibration in time-of-flight MS. The Au is monoisotopic, thus the determination of accurate mass is straightforward and fast compared to common calibration standards like peptides/proteins etc. 2) Use of Au-NP as matrix: the Au-NP (e.g. in the form of flower-like Au-NP) enhance the ionization of complex biomolecules. This enhancement is presumably due to SALDI (Surface Assisted Laser Desorption Ionization) or NALDI (Nano-particles Assisted LDI) effects. Even the combination of classical MALDI matrix with Au-NP offers significant advantages as it was recently reported [1]. 3) Au-NP in MS analysis of intact cells: gold is highly biocompatible; Au-NP can be easily absorbed by endocytosis to cell cytoplasm and increase the signal intensity of peptides and proteins during MS analysis. 4) Tissue imaging: similarly to Au-NP-mediated intact-cell MS, treatment of tissue sections with Au-NP-enriched matrices positively affects the overall quality of mass spectra and improves the tissue MS analysis. Concluding, the use of Au-NP brings new and exciting possibilities in MS of biomolecules.
ER  -

KOLÁŘOVÁ, Lenka, Lukáš KUČERA, Lubomír PROKEŠ, Petr VAŇHARA, Aleš HAMPL a Josef HAVEL. Nano-gold in time-of-flight mass spectrometry of biological samples. In \textit{In International conference on nanotechnology and nanosciences NN16}. 2016.

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