In situ generation of high Aum clusters ions for mass calibration

KOLÁŘOVÁ, Lenka; Lubomír PROKEŠ; Petr VAŇHARA; Cristina FONT CALVARONS; Victoria SALVADO; Aleš HAMPL and Josef HAVEL. In situ generation of high Aum clusters ions for mass calibration. In 68. sjezd chemických společností, 4.-7. září 2016, Praha, Česká republika, No. 1Po08. 2016. ISSN 2336-7202.

Basic information

Original name

In situ generation of high Aum clusters ions for mass calibration

Authors

KOLÁŘOVÁ, Lenka (203 Czech Republic, belonging to the institution); Lubomír PROKEŠ (203 Czech Republic, belonging to the institution); Petr VAŇHARA (203 Czech Republic, belonging to the institution); Cristina FONT CALVARONS (724 Spain); Victoria SALVADO (724 Spain); Aleš HAMPL (203 Czech Republic, belonging to the institution) and Josef HAVEL (203 Czech Republic, guarantor, belonging to the institution)

Edition

68. sjezd chemických společností, 4.-7. září 2016, Praha, Česká republika, No. 1Po08. 2016

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Other information

Language

English

Type of outcome

Conference abstract

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

References:

URL

RIV identification code

RIV/00216224:14310/16:00095854

Organization unit

Faculty of Science

ISSN

Keywords in English

Gold clusters; calibration; mass spectrometry

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Abstract

In the original language

Gold clusters can be generated by Laser Desorption Ionization (LDI) of various gold nanomaterials e.g. gold nano-particles (AuNPs) of various geometry, or gold foil. In our study, Aum clusters generated from flower-like AuNPs were used for internal calibration of peptides1. A simple and effective method for in situ generation of high and stable gold cluster ions (Aum+ and Aum-) were developed. Then, the application of these clusters in external/internal calibration of mass spectra in both positive and negative ion modes was evaluated. The high efficiency of generation of clusters from AuNPs up to m/z 50002-3 has also been demonstrated although these high-mass gold clusters can be easily decomposed to low-mass clusters or even they can explode. Therefore, we aimed 1) to stabilize AuNPs by optimizing the matrices and the use of additives, 2) to extend the mass range towards high masses and 3) to obtain peaks with sufficient intensity and resolution to be usable for internal/external calibration of TOF or Q-TOF mass spectrometers. Among the different additives examined, citric acid/ammonium citrate buffer stabilizes AuNPs in aqueous solution and allows a reproducible generation of high-mass Aum clusters presenting high peak intensities and good resolution. We developed a simple and fast LDI method for the generation of gold clusters. These clusters present peaks, across the whole mass range up to m/z 17 000 in positive and up to m/z 6 000 in negative ion mode, with sufficient signal to noise ratios without using interfering matrices. Our results demonstrated that gold cluster ions can be used in precise in situ calibration in both low and high mass range. This calibration that can be carried out over high mass range without the use of interfering matrices is particularly suitable for MS of peptides and small proteins as well as for low molecular compounds such as metabolites, resulting in the development of more rapid and precise MS methods in biomedical research and diagnostics.