Are there multiple mechanisms of anaerobic sulfur oxidation
with ferric iron in Acidithiobacillus ferrooxidans?

J 2016

Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK, Martin MANDL et. al.

Základní údaje

Originální název

Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?

Název česky

Existuje více mechanismů anaerobní oxidace síry železitými ionty u bakterie Acidithiobacillus ferrooxidans?

Autoři

KUČERA, Jiří (203 Česká republika, domácí), Eva PAKOSTOVÁ (203 Česká republika, domácí), Jan LOCHMAN (203 Česká republika, domácí), Oldřich JANICZEK (203 Česká republika, domácí) a Martin MANDL (203 Česká republika, garant, domácí)

Vydání

Research in Microbiology, Amsterdam, Elsevier, 2016, 0923-2508

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.549

Kód RIV

RIV/00216224:14310/16:00087982

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1016/j.resmic.2016.02.004

UT WoS

000378671300003

Klíčová slova česky

Acidithiobacillus ferrooxidans; anaerobní respirační dráha; sirný metabolismus; redukce Fe(III); RT qPCR

Klíčová slova anglicky

Acidithiobacillus ferrooxidans; Anaerobic respiration pathway; Sulfur metabolism; Ferric iron reduction; RT qPCR

Štítky

AKR, rivok

Změněno: 30. 3. 2017 10:24, Ing. Andrea Mikešková

Anotace

ORIG CZ

V originále

To clarify the pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253 cells, we monitored their energy metabolism gene transcript profiles. Several genes encoding electron transporters involved in aerobic iron and sulfur respiration were induced during anaerobic growth of ferrous iron-grown cells. Most sulfur metabolism genes were either expressed at the basal level or their expression declined. However, transcript levels of genes assumed to be responsible for processing of elemental sulfur and other sulfur intermediates were elevated at the beginning of the growth period. In contrast, genes with predicted functions in formation of hydrogen sulfide and sulfate were significantly repressed. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase); periplasmic electron shuttle rusticyanin; c4-type cytochrome CycA1; the inner membrane cytochrome bc1 complex I; and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. However, an alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Our results conflict with findings regarding the type strain, indicating strain- or phenotype-dependent pathway variation.

Česky

Studium anaerobní bakteriální oxidace síry železitými ionty. Na základě transkriptomických analýz se předpokládá přítomnost složek systému oxidujícího Fe(II), jež katalyzují i anaerobní proces.

Návaznosti

GP14-27075P, projekt VaV

Název: Mechanismus bakteriální anaerobní oxidace síry ovlivňující acidifikaci prostředí sulfidových odpadů

Investor: Grantová agentura ČR, Mechanismus bakteriální anaerobní oxidace síry ovlivňující acidifikaci prostředí sulfidových odpadů

Citovat

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK a Martin MANDL. Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans? Research in Microbiology. Amsterdam: Elsevier, 2016, roč. 167, č. 5, s. 357-366. ISSN 0923-2508. Dostupné z: https://dx.doi.org/10.1016/j.resmic.2016.02.004.

@article{1347637,
   author = {Kučera, Jiří and Pakostová, Eva and Lochman, Jan and Janiczek, Oldřich and Mandl, Martin},
   article_location = {Amsterdam},
   article_number = {5},
   doi = {http://dx.doi.org/10.1016/j.resmic.2016.02.004},
   keywords = {Acidithiobacillus ferrooxidans; Anaerobic respiration pathway; Sulfur metabolism; Ferric iron reduction; RT qPCR},
   language = {eng},
   issn = {0923-2508},
   journal = {Research in Microbiology},
   title = {Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?},
   volume = {167},
   year = {2016}
}

TY  - JOUR
ID  - 1347637
AU  - Kučera, Jiří - Pakostová, Eva - Lochman, Jan - Janiczek, Oldřich - Mandl, Martin
PY  - 2016
TI  - Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans?
JF  - Research in Microbiology
VL  - 167
IS  - 5
SP  - 357-366
EP  - 357-366
PB  - Elsevier
SN  - 09232508
KW  - Acidithiobacillus ferrooxidans
KW  - Anaerobic respiration pathway
KW  - Sulfur metabolism
KW  - Ferric iron reduction
KW  - RT qPCR
N2  - To clarify the pathway of anaerobic sulfur oxidation coupled with dissimilatory ferric iron reduction in Acidithiobacillus ferrooxidans strain CCM 4253 cells, we monitored their energy metabolism gene transcript profiles. Several genes encoding electron transporters involved in aerobic iron and sulfur respiration were induced during anaerobic growth of ferrous iron-grown cells. Most sulfur metabolism genes were either expressed at the basal level or their expression declined. However, transcript levels of genes assumed to be responsible for processing of elemental sulfur and other sulfur intermediates were elevated at the beginning of the growth period. In contrast, genes with predicted functions in formation of hydrogen sulfide and sulfate were significantly repressed. The main proposed mechanism involves: outer membrane protein Cyc2 (assumed to function as a terminal ferric iron reductase); periplasmic electron shuttle rusticyanin; c4-type cytochrome CycA1; the inner membrane cytochrome bc1 complex I; and the quinone pool providing connection to the sulfur metabolism machinery, consisting of heterodisulfide reductase, thiosulfate:quinone oxidoreductase and tetrathionate hydrolase. However, an alternative mechanism seems to involve a high potential iron-sulfur protein Hip, c4-type cytochrome CycA2 and inner membrane cytochrome bc1 complex II. Our results conflict with findings regarding the type strain, indicating strain- or phenotype-dependent pathway variation.
ER  -

KUČERA, Jiří, Eva PAKOSTOVÁ, Jan LOCHMAN, Oldřich JANICZEK a Martin MANDL. Are there multiple mechanisms of anaerobic sulfur oxidation with ferric iron in Acidithiobacillus ferrooxidans? \textit{Research in Microbiology}. Amsterdam: Elsevier, 2016, roč.~167, č.~5, s.~357-366. ISSN~0923-2508. Dostupné z: https://dx.doi.org/10.1016/j.resmic.2016.02.004.

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