2016
Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis
PERNISOVÁ, Markéta; Tomáš PRÁT; Peter GRONES; Danka HARUŠTIAKOVÁ; Martina MATONOHOVÁ et al.Základní údaje
Originální název
Cytokinins influence root gravitropism via differential regulation of auxin transporter expression and localization in Arabidopsis
Autoři
PERNISOVÁ, Markéta; Tomáš PRÁT; Peter GRONES; Danka HARUŠTIAKOVÁ ORCID; Martina MATONOHOVÁ; Lukáš SPÍCHAL; Tomasz NODZYNSKI; Jiří FRIML a Jan HEJÁTKO ORCID
Vydání
New Phytologist, Hoboken, Wiley-Blackwell, 2016, 0028-646X
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10600 1.6 Biological sciences
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 7.330
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14740/16:00088005
Organizační jednotka
Středoevropský technologický institut
UT WoS
EID Scopus
Klíčová slova anglicky
Arabidopsis; AUX1; auxin; cytokinins; PIN-FORMED (PIN); root gravitropism
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 28. 11. 2022 20:29, Mgr. Markéta Pernisová, Ph.D.
Anotace
V originále
• Redirection of intercellular auxin fluxes via relocalization of the PIN3 and PIN7 auxin efflux carriers has been suggested to be necessary for the root gravitropic response. Cytokinins have also been proposed to play a role in controlling root gravitropism, but conclusive evidence is lacking. • We present a detailed study of the dynamics of root bending early after gravistimulation, which revealed a delayed gravitropic response in transgenic lines with depleted endogenous cytokinins (Pro35S:AtCKX) and cytokinin signaling mutants. Pro35S:AtCKX lines as well as a cytokinin receptor mutant ahk3 showed aberrations in the auxin response distribution in columella cells consistent with defects in the auxin transport machinery. • Using in vivo real-time imaging of PIN3-GFP and PIN7-GFP in AtCKX3 overexpression and ahk3 backgrounds, we observed wild type-like relocalization of PIN proteins in columella early after gravistimulation, with gravity-induced relocalization of PIN7 faster than that of PIN3. Nonetheless, the cellular distribution of PIN3 and PIN7 and expression of PIN7 and the auxin influx carrier AUX1 was affected in AtCKX overexpression lines. • Based on the retained cytokinin sensitivity in pin3 pin4 pin7 mutant, we propose the AUX1-mediated auxin transport rather than columella-located PIN proteins as a target of endogenous cytokinins in the control of root gravitropism.
Návaznosti
| GAP501/11/1150, projekt VaV |
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| GA13-40637S, projekt VaV |
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| GP14-30004P, projekt VaV |
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| LH14104, projekt VaV |
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| LQ1601, projekt VaV |
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